Browse > Article
http://dx.doi.org/10.5657/KFAS.2012.0154

Biochemical Changes in the Hemolymph and Hepatopancreas of Abalone Haliotis discus hannai Exposed to Copper  

Park, Hee-Ju (Department of Aquatic Life Medicine, Pukyong National University)
Kang, Ju-Chan (Department of Aquatic Life Medicine, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.45, no.2, 2012 , pp. 154-160 More about this Journal
Abstract
This study the effects of Cu exposure on biochemical factors in the hemolymph and hepatopancreas of the abalone $Haliotis$ $discus$ $hannai$. Abalone were exposed to 0, 5, 10, 20 and 40 ${\mu}g/L$ Cu for 4 weeks. The calcium concentrations in hemolymph were decreased significantly on exposure to 20 and 40 ${\mu}g/L$ Cu after 2 weeks. The aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities in abalone hemolymph were markedly elevated after exposure to 40 ${\mu}g/L$ Cu for 4 weeks. The hepatopancreas superoxide dismutase (SOD) and catalase (CAT) activities were also significantly increased by exposure to 20 ${\mu}g/L$ Cu for 4 weeks. These biochemical factors may represent a convenient method of monitoring heavy metal pollution in coastal areas. From these results, we conclude that a high copper concentration (40 ${\mu}g/L$) in water may curtail hemolymph homeostasis and anti-oxidative reactions in abalone.
Keywords
Cu; Abalone; Hemolymph; Hepatopancreas;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Minghetti M, Leavera MJ, Carpeneb E and Georgea SG. 2008. Copper transporter 1, metallothionein and glutathione reductase genes are differentially expressed in tissues of sea bream (Sparus aurata) after exposure to dietary or waterborne copper. Toxicol Appl Pharm Volume 147 Issue 4, 450-459.
2 Mcgeer JC, Szebedinszky C, Mcdonaldm DG and Wood CM. 2000. Effects of chronic sublethal exposure to waterborne Cu, Cd or Zn in rainbow trout. 1: Iono-regulatory disturbance and metabolic costs. Aquat Toxicol 50, 231-243.   DOI
3 Moody CS and Hassan HM. 1982. Mutagenicity of oxygen free radicals. Proc Natl Acad SCI 79, 2855-2859.
4 Nebot C, Moutet M, Huet P, Xu JZ, Yadan JC. and Chaudiere J. 1993. Spectophotometric assay of superoxide dismutase activity basedon the activited autoxidation of a tetracyclic catechol. Anal Biochem 214, 442-451.   DOI
5 Blasco J and Puppo J. 1999. Effect of heavy metals (Cu, Cd and Pb) on aspartate and alanine aminotransferase in Ruditapes philippinarum (Mollusca: Bivalvia) Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 253-263.
6 Boge G, Bussiere D and Peres G. 1988. Effets du chrome hexavalent sur les fonctions de la bordure en brosse de l'intestin de la truite arc en ciel (Salmo gairdneri R.) Water Research, 441-447.
7 Chance B, Sice H and Boveris A. 1979. Hydroperoxide metabolism in mamamalian organs. Physiil Rev, 527-605.
8 Cheng TC. 1981. Bivalves. In: Inveterbrate Blood Cell. Ratcliffe NA and Rowley AF, eds. Academic Press. London, 233-300.
9 Cheng W and Wang CH. 2001. The susceptibility of the giant freshwater prawn Macrobrachium rosenbergii to Lactococcus parvieae and its resistance under copper sulfate stress. Dis Aquat Organ 47, 137-144.   DOI
10 Exon JH and Koller LD. 1986. Immunotoxicity for cadmium: In: Handbook of Experimental Pharmacology. Cadmium. Foulkes EC, ed. Berlin Heidelberg, Springer-Verlag. 339-350.
11 Forman HJ and Fridovich I. 1973. Superoxide dismutase: A comparison of rate constant. Arch Biochem Biophys 158, 396.   DOI
12 George H and Norman L. 1973. Enzymatic activity in hepatopancreas of Nassarius obsoletus. Comp Biochem Phys B, 153-157.
13 Goldberg B. and Stern A. 1977. The role of the superoxide anion as a toxic species in the erythrocyte. Arch Biochem Biophys, 218-225.
14 Johnson JK, Rocheleau TA, Hillyer JF, Chen CC, Li J and Christensen BM. 2003. A otential role for phenylalanine hydroxylase in mosquito immune responses. Inspect. Biochem Mol Biol 33, 345-354.   DOI
15 Jee JH, Kim SG and Kang JC. 2004. Effects of phenanthrene on growth and basic physiological functions of the olive flounder, Paralichthys olivaceus. J Exp Mar Biol Ecol 304, 123-136.   DOI
16 Abdel-Moati M. 1992. Biochemical and physiologycal responses of Mytilus edulis to Hg and Pb in the coastal waters of Alexandria region. Proceeding of the FAD-UNEP-IOC workshop on biological effects of pollutants in marine organisms. Athens, Greece 69, 61-62.
17 Arai T, Maeda M, Yamakawa H, Kamatani A and Miyazaki N. 2002. Growth effect on the uptake and elimination of trace metals in the abalones Haliotis. Fisheries Sci 68, 1094-1098.   DOI
18 Ashida M, Soderhall K. 1984. The prophenoloxidase activating system in crayfish. Comp Biochem Physiol 77, B21-6.
19 Barry H and John MCG. 1995. The definition and measurement of antioxidants in biological systems Free Radical Biology and Medicine, 125-126.
20 Wong PPK, Chu LM and Wong CK. 1999. Study of toxicity and bioaccumulation of copper in the silver sea bream Sparus sarba. Environ Int 25, 417-422.   DOI
21 Zar JH. 1996. Biostatistical Analysis. Prentice Hall, London, 662.
22 Parihar MS, Javeri T, Hemnani T, Dubey AK and Parkash P. 1997. Response of superoxide dismutase, glutathion peroxidase and reduced glutathion antioxidant defenses in gills of the freshwater catfish (Heteropneustes fossilis) to short-term elevated temperature. J Therm Biol 22, 151-156.   DOI
23 Pipe RK, Coles JA, Carissan FMM and Ramanathan K. 1999. Copper induced immunomodulation in the marine mussel, Mytilus edulis. Aquqt Toxicol 46, 43-54.   DOI
24 Rishan CS, Wenjie L, Guangxing F, Tingjun, M. xianghong, Lingling Y and Liyan Z. 2005. Purification and characterization of phenoloxidase from clam, Ruditapes philippinarum. Fish Shell Immuno 18, 61-70.   DOI
25 Sherba M, Dunham DW and Harvey HH. 2000. Sublethal copper toxicity and food response in the freshwater crayfish, Cambarus bartonii (Cambaridae, Decapoda, Crustacea). Ecotoxicol Environ Saf 46, 329-333.   DOI
26 Simon RH, Scoggin CH and Patterson D. 1981. Hydrogen peroxide causes the fatal injury to human fibroblasts exposed to oxygen radicals. J Biol Chem 256, 7181-7186.
27 Sutherland J and Major CW. 1981. Internal heavy metal changes as a consequence of exposure of Mytilus edulis, the blue mussel, to elevated external copper (II) levels. Comp Biochem Physiol, 63-67.
28 Viarengo A, Pertic M, Mancinelli G, Burlundo B, Canesi JL and Orunesu M. 1996. In Vivo effects of copper on the calcium homeostasis mechanisms of mussel gill cell plasma membranes. Comp Biochem Phys B 113, 421-425.   DOI
29 Weifang W, Kangsen M, Wenbing Z, Qinghui A, Chunfeng Y, Huitao L and Zhiguo L. 2009. Effects of diertry copper on survival, growth and immune response of juvenile abalone, Haliotis discus hannai. Ino Aquaculture 297, 122-127.   DOI
30 Wendel A. and Feuerstein S. 1981. Drug-induced lipid peroxidation in mice-1. Modulation by monoxygenase activity, glutathione and selenium status. Biochem Pharmacol 30, 2513-2520.   DOI
31 Winston GW and Di Giulio RT, 1991. Pro-oxidant and antioxidant mechanisms in aquatic organsm. Aquat Toxicol 19, 137-161.   DOI
32 Kawai K. 1959. The cytochrome system in marine lamellibranch tissues. Biol Bull 117, 125-132.   DOI
33 Kim JH, Mok JS and Park HY. 2005. Trace Metal Contents in Seaweeds from Korean Coastal Area. J. Korean Soc Food Sci Nutr 34, 1041-1051.   DOI
34 Koyama J. and Itazawa Y. 1977. Effects of oral administration of cadmuim on fish: I. Analutical results of the blood and bones. Bull Jap Soc Sci Fish 43, 523-526.   DOI
35 Lee IS and Kim EJ. 2000. Distribution of Heavy Metals in Sediments, Seawater and Oysters (Crassostrea gigas) in the Jinhae Bay. J Ecology 23, 59-64.
36 Lopes PA, Pinheiro T, Mathias MC, Collares-Pereira MJ and Viegas- Crespo, AM. 2001. Response of antioxidant enzymee in freshwater fish population (Leuciscus alburnoides complex) to inorganic pollutants exposure. Sci Total Environ 280, 153-163.   DOI
37 Marr JCA, Lipton J, Cacela D, Hansen JA, Bergman HL, Meyer JS. and Hogstrand C. 1996. Realtionships between copper exposure duration, tissue copper concentration, and rainbow trout growth. Aquat Toxicol 36, 17-30.   DOI