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http://dx.doi.org/10.5657/KFAS.2011.0207

Characterization of Organochlorine Insecticide Endosulfan-Degrading Bacterium Isolated from Seaside Sediment  

Park, Mi-Eun (Department of Ecological Engineering, Pukyong National University)
Kim, Young-Mog (Department of Food Science and Technology, Pukyong National University)
Chung, Yong-Hyun (Department of Ecological Engineering, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.44, no.3, 2011 , pp. 207-215 More about this Journal
Abstract
An endosulfan degrading bacterial strain, K-1321, was isolated by endosulfan-enrichment culture from a seaside sediment collected at Dadaepo Beach, Busan, Korea. The strain was identified as a Serratia sp. based on the results of morphological, biochemical and 16S rDNA homology analyses. Serratia sp. K-1321 was able to completely degrade 50 ppm endosulfan in culture media and soil within 6 weeks at $25^{\circ}C$. GC/MS analysis revealed that endosulfan diol was an intermediate of the bacterial endosulfan degradation. Considering the above results, we concluded that Serratia sp. K-1321 utilized endosulfan as a carbon source and metabolized endosulfan via a less toxic pathway, such as the formation of endosulfan diol as an intermediate.
Keywords
Bioremediation; Endosulfan; Organochlorine; Seaside sediment;
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1 Park KR, Jung YS and Rim H. 2008. A case of endosulfan intoxication with pulmonary embolism. Kor Assoc Intern Med 74, S250-S253.
2 Park SK, Ha KT, Kim SD, Kim SJ, Lee YJ, Lee KA, Jang JI, Jeoung SS, Song BY, Jo SJ, Jo HB, Choi BH and Kim MY. 2009. Survey on pesticide residues in commercial agricultural products in the northern area of Seoul. J Food Hyg Safety 24, 27-37.   과학기술학회마을
3 Rao DMR and Murty AS. 1980. Persistence of endosulfan in soils. J Agric Food Chem 28, 1099-1101.   DOI
4 Shin JH, Kwak YY, Kim WC, So JH, Shin HS, Park JW, Kim TH, Kim JE and Rhee IK. 2008. Isolation of endosulfan degrading bacteria and their degradation characteristics. Kor J Environ Agric 27, 292-297.   과학기술학회마을   DOI
5 Siddique T, Okeke BC, Arshad M and Frankenberger WT. 2003. Enrichment and isolation of endosulfan-degrading microorganisms. J Environ Qual 32, 47-54.   DOI
6 Hussain S, Arshad M, Saleem M and Khalid A. 2007. Biodegradation of alpha- and beta-endosulfan by soil bacteria. Biodegradation 18, 731-740.   DOI
7 Kim SD, Kim BS, Park SK, Kim MY, Cho TH, Han CH, Jo HB and Choi BH. 2007. A Study of current status on pesticide residues in commercial dried agricultural products. Kor J Food Sci Technol 39, 114-121.   과학기술학회마을
8 Kim SK, Cho SH and Cho NS. 2004a. A case of endosulfan intoxication occurring in groups. Kor Soc Emerg Med 5, 409-412.
9 Kim TK, Kim JE, Kim TH, Shin HS, Kwon OK, Kim JD and Lim SJ. 2008. Chemical remediation of soils contaminated with endosulfan organochlorine insecticide. Kor J Environ Agric 2, 75-89.
10 Kim YG, Kim TG, Park SS, Heo NC and Hong SS. 2000. A study on residual pesticides in commercial fruits and vegetables. Kor J Food Sci Technol 32, 763-771.   과학기술학회마을
11 Kim YM, Park K, Joo GJ, Jeong EM, Kim JE and Rhee IK. 2004b. Glutathione-dependent biotransformation of the fungicide chlorothalonil. J Agric Food Chem 52, 4192-4196.   DOI
12 Krieg NR and Holt JG. 1984. Bergey's Manual of Systematic Bacteriology Vol 1. In: Genus VIII. Grimont PAD and Grimont F, eds. Williams & Wilkins, Baltimore, U.S.A., 477-484.
13 Kwon GS, Kim JE, Kim TK, Sohn HY, Koh SC, Shin KS and Kim DG. 2003. Klebsiella pneumoniae KE-1 degrades endosulfan without formation of the toxic metabolite, endosulfan sulfate. FEMS Microbiol Lett 215, 255-259.
14 Kwon GS, Sohn HY, Shin KS, Kim EJ and Seo BI. 2005. Biodegradation of the organochlorine insecticide, endosulfan, and the toxic metabolite, endosulfan sulfate, by Klebsiella oxytoca KE-8., Appl Microbiol Biotechnol 67, 845-850.   DOI
15 Berns KI and Thomas CA. 1965. Isolation of the high molecular DNA from Haemophilus influenzae. J Mol Biol 11, 476-490.   DOI
16 Cho KB. 2010. Residual Characteristics of endosulfan in Busan and Gimhae Farmland. M.D. Thesis, Dong-A University, Busan, Korea.
17 Chung RP, Choi MK, Yeo HG and Chun MY. 2001. Seasonal variations in the concentration of persistent organochlorine pesticides in atmosphere. Kor J Environ Agric 20, 79-85   과학기술학회마을
18 Chaudhuri K, Selvaraj S and Pal AK. 1999. Studies on the genotocixity of endosulfan in bacterial systems. Mutat Res 439, 63-67.   DOI   ScienceOn
19 Beauvais SL, Silva MH and Powell S. 2010. Human health risk assessment of endosulfan: III. Occupational handler exposure and risk. Regul Toxicol Pharmacol 56, 28-37.   DOI
20 Awasthi N, Singh AK, Jain RK, Khangarot BS and Kumar A. 2003. Degradation and detoxification of endosulfan isomers by a defined co-culture of two Bacillus strain, Appl Microbiol Biotechnol 62, 279-283.   DOI
21 Sutherland TD, Horne I, Russell RJ and Oakeshott JG. 2002b. Gene cloning and molecular characterization of a two-enzyme system catalyzing the oxidative detoxification of beta-endosulfan. Appl Environ Microbiol 68, 6237-6245.   DOI
22 Silva MH and Beauvais SL. 2010. Human health risk assessment of endosulfan: I. Toxicology and hazard identification. Regul Toxicol Pharmacol 56, 4-17.   DOI
23 Silva MH and Carr WC. 2010. Human health risk assessment of endosulfan: II. Dietary exposure assessment. Regul Toxicol Pharmacol 56, 18-27.   DOI
24 Sutherland TD, Horne I, Harcourt RL, Russell RJ and Oakeshott JG. 2002a. Isolation and characterization of a Mycobacterium strain that metabolizes the insecticide endosulfan. J Appl Microbiol 93, 380-389.   DOI   ScienceOn
25 Usha S and Harikrishnan VR. 2004. Endosulfan, Thanal, Kerala, India, 3-4.
26 Lo'pez-Blanco MC, Cancho-Grande B, Simal-Ga'ndara J, Lo'pez-Periago E and Arias-Estevez M. 2005.Transport of commercial endosulfan through a column of aggregated vineyard soil by a water flux simulating field conditions. J Agric Food Chem 53, 6798-6743.   DOI
27 Laabs V, Amelung W, Pinto AA, Wantzen M, da Silva CJ and Zech W. 2002. Pesticides in surface water, sediment, and rainfall of the northeastern Pantanal basin, Brasil. J Environ Qual 31, 1636-1648.   DOI
28 Lee JB, Sohn HY, Shin KS, Jo MS, Kim JE, Lee SW, Shin JW, Kum EJ and Kwon GS. 2006. Isolation of a soil bacterium capable of biodegradation and detoxification of endosulfan and endosulfan sulfate. J Agric Food Chem 54, 8824-8828.   DOI
29 Lee SE, Kim JS, Kennedy IR, Park JW, Kwon GS, Koh SC and Kim JE. 2003. Biotransformation of an organochlorine insecticide, endosulfan, by Anabaena species. J Agric Food Chem 51, 1336-1340.   DOI
30 Dunbar J, Ticknor LO and Kuske CR. 2000. Assessment of microbial diversity in four South-western United States soils by 16S rRNA gene terminal restriction fragment analysis. Appl Environ Microbiol 66, 2943-2950.   DOI
31 Fujii K, Urano N, Kimura S, Nomura Y and Karube I. 2000. Microbial degradation of nonylphenol in some aquatic environments. Fish Sci 66, 44-48.   DOI
32 Guerin TF. 2001. A biological loss of endosulfan and related chlorinated organic compounds form aqueous systems in the presence and absence of oxygen. Environ Pollut 115, 219-230.   DOI
33 Ha KT, Park SK, Cho TH, Han CH, Kim SD, Lee KA, Kim SJ, Jang JI, Jo HB and Choi BH. 2009. Monitoring on endocrine disruptors in commercial agricultural products in the northern area of Seoul (2007). J Food Hyg Safety 24, 136-142.
34 Harner T, Shoeib M, Kozma M, Gobas FAPC and Li SM. 2005. Hexachlorocyclohexanes and endosulfans in urban, rural, and high altitude air samples in the fraser valley, british columbia: Evidence for trans-pacific transport. Environ Sci Technol 39, 724-731.   DOI