Browse > Article
http://dx.doi.org/10.5657/kfas.2010.43.5.428

Hepatic Detoxification and Antioxidant Activity in Sea-urchin Roe and Ethanol Extract of Roe  

Lee, Seung-Joo (Department of Food Processing and Distribution, Gangneung-Wonju National University)
Ha, Wang-Hyun (Department of Food Processing and Distribution, Gangneung-Wonju National University)
Choi, Hye-Jin (Department of Food Processing and Distribution, Gangneung-Wonju National University)
Cho, Soon-Yeong (Department of Food Processing and Distribution, Gangneung-Wonju National University)
Choi, Jong-Won (College of Pharmacy, Kyungsung University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.43, no.5, 2010 , pp. 428-436 More about this Journal
Abstract
Sea-urchins (Anthocidaris crassispina) are widely distributed in the East Sea of Korea. The aim of this study was to evaluate the hepatoprotective effects of sea-urchin roe on bromobenzene (BB)-induced liver damage in rats. The antioxidative and detoxifying properties of sea-urchin roe in BB-poisoned rat liver was examined by chemical analysis of serum aminotransferase (AST, ALT), glutathione S-transferase (GST), $\gamma$-glutamylcystein synthetase, glutathione reductase, epoxide hydrolase, amino-N-demethylase (AD), aniline hydrolase (AH) enzyme activity, as well as lipid peroxide and glutathione contents. Sea-urchin roe inhibited the increase of serum AST, ALT enzyme activity. Increasing lipid peroxide contents and AD and AH activities were significantly decreased in ethanol extract of sea-urchin roe. GST, $\gamma$-glutamylcystein synthetase, glutathione reductase and epoxide hydrolase enzyme activities increased in sea-urchin roe-fed group, compared with the BB-treated group. These results suggest that sea-urchin roe facilitates recovery from liver damage by enhancing antioxidative defense mechanisms and hepatic detoxication metabolism.
Keywords
Sea-urchin roe; Antioxidative; Detoxication;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Reitman S and Frankel S. 1957. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. American Journal of clinical Pathology 28, 58-63.
2 Ryo, YG and Park DW. 1986. Studies on artificial seeding production of sea urchin Anthocidaris crassispina (A.Agasiz). Bull Fish Res Dev Agency 39, 89-96.
3 Wui IS, Lee JB and Yoo SH. 1992. Bioassay on marine sediment pollution by using sea urchin embryo culture in the south-west island sea of Korea. Korean J Environ Biol 10, 92-99.
4 Yoo SK, Hur S and Ryu HY. 1982. Growth and spawning of the sea urchin Anthocidaris crassispina (A. Agassiz). Bull Korean Fish Soc 15, 345-358.   과학기술학회마을
5 Yoon JK, Kim GJ and Shin JG. 1997. Effect of the equation of the equinoxes on the liver damage induced with bromobenzene in rats. J Korean Toxicol 13, 377-383.
6 Kato R. 1977. Drug metabolism under pathological and abnormal physiological states in animals and man. Xenobiotica 7, 25-92.   DOI
7 Kim EJ, Lee CK and Choi JW. 1992. The effect of Scoparone on the hepatic bromobenzene metabolizing enzyme system in rats. Kor J Pharmacogn 23, 81-82.   과학기술학회마을
8 Kim SY, Jung JY, Kim JR and Kim JH. 1994. The change of glutathione metabolism in liver and kidney of cisplatin treated rats. The Yeungnam Med J 11, 2.
9 Korea Agro-Fisheries Trade Corp. 2000. Korea agricultural trade information : Production statistics.
10 Lee HJ, Kim GH and Lee EO. 2003. Hepatoprotective effect of the methanol fraction of chinese cabbage on liver injury in rats treated by bromobenzene. Korean J Oriental Physiology and Pathology 17, 1177-1181.   과학기술학회마을
11 Litwack G, Ketterer B and Aris IM. 1971. A hepatic protein which binds steroids, bilirubin, corcinogens, and a number of exogenous anions. Nature 234, 466-467.   DOI
12 Lowry OH, Rosebrough NJ, Farr AL and Randall RJ. 1951. Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193,265-275.
13 Mitchell JR and Hornig MG. 1984. Drug metabolism and drug toxicology. Raven press 23-25.
14 Nam HK. 1986. The composition of fatty acid and amino acid for sea urchin. J Korean Oil Chemists' Soc 3, 33-37.   과학기술학회마을
15 Nash T. 1953. The colorimetric estimarion of formaldehyde by means of the hentisch reaction. J of Biological Chemistry 55, 412-416.
16 Oh JD. 2003. Effect of dietary Monascus Koji on the liver damage induced with bromobenzene in rats. Korean Soc Food Sci Nutr 33, 965-972.   과학기술학회마을   DOI
17 Bidlack WR and Lowry GL. 1982. Multiple drug metabolism: p-nitroanisole reversal of acetone enhanced aniline hydroxylation. Biochemical Pharmacology 31, 311-317.   DOI
18 Boyland E and Chasseud LF. 1969. The role of glutathione and glutathione S-transferases in mercapturic acid biosynthesis. Advances in Enzymolgy and Related Areas of Molecular Biology 32, 173-219.
19 Casini AF, Ferrali M, Pompella A, Maellaro E and Comporti M. 1986. Lipid peroxidation and cellular damage in extra-hepatic tissues of bromobenzeneintoxicated mice. Am J Pathol 123, 520-531.
20 De la Cruz-Garcia C, Lopez-Hernandez J, Gonzalez- Castro M, Rodriguez-Bernaldo J, De Qiros A and Simal-Lozano J. 2000. Protein, amino acid and fatty acid contents in raw and canned sea urchin (Paracentrotus lividus) harvested in Galicia (NW Spain). J Sci Food Agric 80, 1189-1192.   DOI
21 Ellman GL. 1959. Tissue sulfhydryl group. Arch Biochem Biophys 82, 70-77.   DOI
22 Habig WH, Pabst MJ and Jakoby WB. 1974. Glutathione S-transferase. The first enzymatic step in mercapturic acid and formation. Journal of Biological Chemistry 249, 7130-7139.
23 Hasegawa LS and Hammock BD. 1982. Spectrophotometric assay for mammalian cytosolic epoxide hydrolase using trans-stillbene oxide as the substrate. Biochem Pharmacol 31, 1979-1984.   DOI
24 Hayashi O, Niki E, Kondo M and Yoshikawa T. 1984. Medical, biochemical and chemical aspects of free radicals. Elsevier Sci Pub 1-9.
25 Heijine WHJ, Slitt AL, van Bladeren PJ, Groten JP, Klaassen CD, Stierum RH and van Ommen B. 2004. Bromobenzene induced hapatotoxicity at the transcriptome level. Toxicol Sci 79, 411-422.   DOI
26 Karmen A. 1995. A note on the spectrophotometric assay of glutamic oxaloacetic transaminase in human blood serum. 1955. Journal of Clinical Investigation 34, 131-133.
27 Zheng J and Hanzlik RP. 1991. Premercapturic acid metabolites of bromobenzene derived via its 2,3- and 3,4-oxide metabolites. Xenobiotica. the Fate of Foreign Compounds in Biological Systems 21, 535-546   DOI   ScienceOn
28 AOAC. 1990. Official Method of Analysis. 15th ed. Association of Official Analytical Chemists. Washington DC. U.S.A. 931-943.
29 Arrick BA and Nathan CF. 1984. Glutathione metabolism as a determinant of therapeutic efficacy. a review. Cancer Res 44, 4224-4232.
30 Yu CM and Cho KA. 1999. The effects of temperature on biological evaluation of sea water with sea urchins. J Korean Environ Sci Soc 8, 160-164.
31 Pryor WA. 1977. Free radical in biology : Involvement of radical reaction in aging and carcinogenesis in medicinal chemistry. Elservier and Amsterdam, 331-361.
32 Ohkawa H, Ohish N and Yaki K. 1979. Assay for lipid peroxide in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry 95, 351-355.   DOI
33 Park JC, Choi JS, Song SH, Choi MR, Kim KY and Choi JW. 1997. Hepatoprotective effect of extracts and phenolic compound from marine algae in bromobenzene-treated rats. Kor J Pharmacogn 28, 239-246.   과학기술학회마을
34 Poulas TL and Raag R. 1992. Cytochrome 450 cam : Crystallography, oxygen activation and elecrontransfer. FASEB J 6, 674-679.   DOI
35 Reid WD, Chistie B, Krishna G, Mitchell JR, Moskowitz J and Brodie BB. 1971. Bromobenzene metabolism and hepatic necrosis. Pharmacology 6, 41-55.   DOI