Browse > Article
http://dx.doi.org/10.5657/kfas.2010.43.4.298

Microbiological Quality of the Shellfish-growing Waters and Mussels in Changseon, Namhae, Korea  

Yoo, Hyun-Duk (Southeast Sea Fisheries Research Institute, National Fisheries Research and Development Institute)
Ha, Kwang-Soo (Southeast Sea Fisheries Research Institute, National Fisheries Research and Development Institute)
Shim, Kil-Bo (Southeast Sea Fisheries Research Institute, National Fisheries Research and Development Institute)
Kang, Jin Yeong (Southeast Sea Fisheries Research Institute, National Fisheries Research and Development Institute)
Lee, Tae-Seek (Southeast Sea Fisheries Research Institute, National Fisheries Research and Development Institute)
Kim, Ji-Hoe (Southeast Sea Fisheries Research Institute, National Fisheries Research and Development Institute)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.43, no.4, 2010 , pp. 298-306 More about this Journal
Abstract
A sanitary survey was conducted to evaluate the water quality and mussel (Mytilus edulis) conditions of two administrative shellfish growing waters: those designated as shellfish growing water for export, and adjacent waters on the east coast of Changseon Island, Namhae, Korea. In all, 1,656 seawater and 166 mussel samples were collected at 46 stations for seawater and five stations for the shellfish from January 2007 to December 2009. Both seawater and mussels were examined for total coliforms and fecal coliforms. The standard plate count and most probable number of Escherichia coli were also determined for the shellfish samples. The range of the geometric means and the estimated 90th percentiles of fecal coliform for seawater samples at each station were <1.8-4.1 MPN/100 mL and <1.8-22.3 MPN/100 mL, respectively. The sanitary conditions for both shellfish growing areas, the designated shellfish growing area and adjacent area in Changseon, met the 'Approved area' criteria of the United States National Shellfish Sanitation Program. The range of E. coli detected in mussels in these areas was <20-500 MPN/100 g; and only 3 of 166 samples exceeded 230 MPN/100 g, the European standard for the consumption of raw bivalves. Therefore, the mussel farms in the Changseon area are classified as 'Class A' according to criteria set in European Community regulations. However, the levels of E. coli in mussels in parts of the designated shellfish growing area and adjacent area exceeded the bacterial limits after rainfall exceeding 45 mm. Further studies are needed to fully define the conditions leading to a temporary closure to harvest after a rainfall event.
Keywords
Shellfish growing area; Sanitary survey; Mussel; Fecal coliform; Escherichia coli;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 ISO (International Organization for Standardization). 2005. ISO/TS 16649-3. Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of beta-glucuronidse-positive Escherichia coli. Part 3: Most probable number technique using 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide. ISO, 8pp.
2 Kim SJ. 1975. Sanitary studies of oysters and growing areas in the south coast of Korea. Bull Fisheries Research & Development Agency 14, 1-79.
3 Kwon JY, Park KBW, Song KC, Lee JH, Park JH, Kim JD and Son KT. 2007. Evaluation of the bacteriological quality of a shellfish-growing area in Kamak Bay, Korea. J Fish Sci Technol 11, 7-14.   과학기술학회마을   DOI
4 Legnani P, Leoni E, Lev D, Rossi R, Villa GC and Bisbini P. 1998. Distribution of indicator bacteria and bacteriophages in shellfish and shellfish growing waters. J Appl Microbiol 85, 790-798.   DOI
5 Mallin MA, Williams KE, Esham EC and Lowe RP. 2000. Effect of human development on bacteriological water quality in coastal watersheds. Ecol Appl 10, 1047-1056 .   DOI
6 Mallin MA, Ensign SH, McIver MP, Shank GC and Fowler PK. 2001. Demographic, landscape, and meteorological factors controlling the microbial pollution of coastal waters. Hydrobiologia 460, 185-193.   DOI
7 NFRDA (National Fisheries Research & Development Agency), 1978. Handbook of the tide current pattern in the coastal growing and heavy industrial areas of Korea. NFRDA, pp. 107-120.
8 Potasman I, Paz A and Odeh M. 2002. Infectious outbreaks associated with bivalve shellfish consumption: A worldwide perspective. Clin Infect Dis 35, 921-928.   DOI
9 Power UF and Collins JK. 1989. Differential depuration of poliovirus, Escherichia coli, and a coliphage by the common mussel, Mytilus edulis. Appl Environ Microbiol 55, 1386-1390.
10 Power UF and Collins JK. 1990. Tissue distribution of a coliphage and Escherichia coli in mussels after contamination and depuration. Appl Environ Microbiol 56, 803-807.
11 APHA (American Public Health Association). 1970. Recommended Procedures for the Examination of Sea Water and Shellfish. 4th ed. American Public Health Association, Inc. Washington, D.C. U.S.A., 1-105.
12 Cliver DO. 1988. Virus transmission via foods. Food Technol 42, 241-248.
13 de Mesquita MMF, Evison LM and West PA. 1991. Removal of faecal indicator bacteria and bacteriophages from the common mussel (Mytilus edulis) under artificial depuration conditions. J Appl Bacteriol 70, 495-501.   DOI
14 European Commission. 2005. Commission Regulation (EC) No 2073/2005 of 15 November 2005 on microbiological criteria for foodstuffs. Off J Eur Union L338, 1-23.
15 European Commission. 2004. Regulation (EC) No 854/2004 of the European Parliament and of the Council of 29 April 2004 laying down specific rules for the organisation of official controls on products of animal origin intended for human consumption. Off J Eur Communities L155, 206-321.
16 Feldhusen F. 2000. The role of seafood in bacterial foodborne diseases. Microbes Infect 2, 1651-1660.   DOI
17 Grimes DJ. 1991. Ecology of estuarine bacteria capable of causing human disease: A review. Estuaries 14, 345-360.   DOI
18 Rippey SR. 1994. Infectious diseases associated with molluscan shellfish consumption. Clin Microbiol Rev 7, 419-425.   DOI
19 Shim KB, Ha KS, Yoo HD, Kim JH and Lee TS. 2009. Evaluation of the bacteriological safety for the shellfish growing area in Jaranman․Saryangdo area, Korea. Kor J Fish Sci 42, 442-448.
20 Song KC, Lee DS, Shim KB, Lim CW, Mog JS, Byun HS, Park YJ and Cho KC. 2008. Evaluation of bacteriological safety for the shellfish growing waters in Taean area, Korea. J Kor Fish Soc 41, 155-162.   과학기술학회마을   DOI
21 U.S. FDA (Food and Drug Administration). 2007. National Shellfish Sanitation Program, Guide for the control of molluscan shellfish, Model ordinance. Retrieved from http://www.fda.gov/Food/FoodSafety/ Product-SpecificInformation/Seafood/FederalStatePr ograms/NationalShellfishSanitationProgram/default.htm on June 18, 2010
22 Ha KS, Shim KB, Yoo HD, Kim JH and Lee TS. 2009. Evaluation of the bacteriological safety for the shellfish growing area in Hansan․Geojeman, Korea. Kor J Fish Aquat Sci 42, 449-455.   과학기술학회마을   DOI