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http://dx.doi.org/10.5657/kfas.2009.42.6.614

Effects of Dietary Supplementation of Alga Mixtures (Hizikia fusiformis and Ecklonia cava) on Innate Immunity and Disease Resistance Against Edwardsiella tarda in Olive Flounder (Paralichthys olivaceus)  

Kim, Sung-Sam (Faculty of Marine Biomedical Science, Jeju National University)
Jang, Ji-Woong (Faculty of Marine Biomedical Science, Jeju National University)
Song, Jin-Woo (Faculty of Marine Biomedical Science, Jeju National University)
Lim, Se-Jin (Faculty of Marine Biomedical Science, Jeju National University)
Jeong, Joon-Bum (Faculty of Marine Biomedical Science, Jeju National University)
Lee, Sang-Min (Faculty of Marine Bioscience and Technology, Kangnung-Wonju National University)
Kim, Kang-Woong (Aquaculture Management Division, National Fisheries Research and Development Institute)
Son, Maeng-Hyun (Aquaculture Management Division, National Fisheries Research and Development Institute)
Lee, Kyeong-Jun (Faculty of Marine Biomedical Science, Jeju National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.42, no.6, 2009 , pp. 614-620 More about this Journal
Abstract
We report non-specific immune responses and its disease resistance against Edwardsiella tarda by alga mixture (HE; Hizikia:Ecklonia) in olive flounder for the first time. Five isonitrogenous (44% crude protein) and isocaloric (17.1 MJ $kg^{-1}$) diets were formulated to have 0%, 2%, 4%, 6% and 8% of the alga mixture. One of five experimental diets was fed triplicate groups of fish (30 fish/group) to apparent satiation in a flow through system. After a two week feeding, blood was sampled at 3, 6, 12, 24 h after the last feeding for a kinetic measurement of nitroblue tetrazolium (NBT) activity and healthy fish with similar sizes in each tank were selected and injected with 1 mL of E. tarda suspension ($1.0\times10^7$ CFU/mL) to evaluate the disease resistance of the fish. Dietary supplementation of alga mixtures resulted in significantly higher non-specific immune responses compared with the fish fed the control diet. The cumulative mortality was significantly lower in the fish groups fed alga mixture containing diets than control group in the challenge test with E. tarda. Therefore, the results in this study indicate that dietary supplementation of Hizikia and Ecklonia mixtures enhance the non-specific immune responses and a disease resistance of olive flounder.
Keywords
Olive flounder; Hizikia fusiformis; Ecklonia cava; Edwardsiella tarda; Immune responses;
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1 Bu HJ, Ham YM, Kim JM, Lee SJ, Hyun JW and Lee NH. 2006. Elastase and hyaluronidase inhi- bition activities of phlorotannins isolated from Ecklonia cava. Kor J Pharmacogn 37, 92-96
2 Cho KJ, Lee YS and Ryu BH. 1990. Antitumor effect and immunology activity of seaweeds toward sarcoma-180. Bull Korean Fish Soc 23, 345- 352
3 Kusuda R, Itami T, Mumekiyo M and Nakajima H. 1997. Characteristics of an Edwardsiella sp. from an epizootic of cultured crimson sea breams. Bull Jpn Soc Scient Fish 43, 129-134
4 Larson RA. 1988. The antioxidants of higher plants. Phytochemistry 27, 969-978   DOI   ScienceOn
5 Park JH, Kang KC, Baek SB, Lee YH and Rhee KS. 1991. Separation of antioxidant compounds from edible marine algae. Korean J Food Sci Technol 23, 256-261
6 Park YB. 2005. Determination of nitrite-scavenging activity of seaweed. J. Korean Soc Food Sci Nutr 34, 1293-1296   DOI   ScienceOn
7 Ruperez P, Ahrazem O and Leal JA. 2002. Potential antioxidant capacity of sulfated polysaccharides from the edible marine brown seaweed Fucus vesiculosus. J Agric Food Chem 50, 840-845   DOI   ScienceOn
8 Lindsay GJH, Walton MJ, Adron JW, Fletcher TC, Cho CY and Cowey CB. 1984. The growth of rainbow trout (Salmo gairdneri) given diets containing chitin and its relationship to chitinolytic enzymes and chitin digestibility. Aquaculture 37, 315-334   DOI   ScienceOn
9 Yoon JA, Yu KW, Jun WJ, Cho HY, Son YS and Yang HC. 2000. Screening of anticoagulant activity in the extracts of edible seaweeds and optimization of extraction condition. J Korean Soc Food Sci Nutr 29, 1098-1106
10 Garling DLJ and Wilson RP. 1976. Optimum dietary protein to energy ratio for channel catfish fingerlings, Ictalurus punctatus. J Nutr 106, 1368-1375   PUBMED
11 Okai Y, Okai KH, Ishizaka S and Yamashita U. 1997. Enhancing effect of polysaccharides from an edible brown alga, Hijikia fusiformes (Hijikia), on release of tumor necrosis factor alpha from macrophages of endotoxin-nonresponder C3H/HeJ mice. Nutr Cancer 27, 74-79   DOI   PUBMED   ScienceOn
12 Park EY and Lee SS. 1996. Effect of dietary fiber on the serum lipid level and bowel function in aged rats. Korean J Nutr 29, 934-942   DOI   ScienceOn
13 Lee HA, Lee SS and Shin HK. 1994. Effect of dietary fiber source on the composition of intestinal microflora in rats. Korean J Nutr 27, 988-995
14 Pham MA, Lee KJ, Lee BJ, Lim SJ, Kim SS, Lee YD, Heo MS and Lee KW. 2006. Effects of dietary Hizikia fusiformis on growth and immune responses in juvenile olive flounder (Paralichthys olivaceus). Asian-Aust. J Anim Sci 19, 1769-1775   DOI
15 Folch J, Lee M and Sloane–Stanley GH. 1959. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226, 497-509
16 Mayer AMS and Hamann MT. 2004. Marine pharmacology in 2000: Marine compounds with antibacterial, anticoagulant, antifungal, anthelmintic, anti-inflammatory, antiplatelet, antiprotozoral and antiviral activities affecting the cardiovascular, endocrine, immune and nervous system, and other miscellaneous mechanisms of action Mar Biotechnol 6, 37-52   DOI   ScienceOn
17 Athukorala Y, Kim KN and Jeon YJ. 2006. Antiproliferative and antioxidant properties of an enzymatic hydrolysate from brown alga, Ecklonia cava. Food and Chemical Toxicology 44, 1065-1074   DOI   ScienceOn
18 Alcaide E, Herraiz S and Esteve C. 2006. Occurrence of Edwardsiella tarda in wild European eels Anguilla anguilla from Mediterranean Spain. Dis Aquat Org, 73, 77-81   DOI   PUBMED
19 AOAC. 1995. Official methods of analysis. 16th ed. Association of Official Analytical Chemists, Arlington, Virginia pp. 1298
20 Haung HL and Wang BG. 2004. Antioxidant capacity and lipophilic content seaweeds collected from the Quandao coastline. J Agric Food Chem 52, 4993-4997   DOI   ScienceOn
21 Shan BE, Yoshida Y, Koroda E and Yamashita U. 1999. Immunomodulating activity of seaweed extract on human lymphocytes in vitro. Int. J Immunopharmaco 21, 59-70   DOI   ScienceOn
22 Anderson DP and Siwicki AK. 1995. Basic haema- tology and serology for fish health programs. In: Diseases in Asian aquaculture Ⅱ. Shariff, M, JR Arthur and RP Subasinghe, eds. Asian Fisheries Society, Manila, Philippines pp. 185
23 Mohanty BR and Sahoo PK. 2007. Edwardsiellosis in fish: a brief review. J Biosci 32, 1331-1344   DOI   PUBMED
24 Lim SN, Cheung PCK, Ooi VEC and Ang PO. 2002. Evaluation of antioxidative activity of extracts from a brown seaweed, Sargassum siliquastrum. J Agric Food Chem 50, 3862-3866   DOI   ScienceOn
25 Okai Y, Okai KH, Ishizaka S, Ohtani K, Yuasa IM and Yamashita U. 1998. Possible immunomodulating activities in an extract of edible brown algae, Hijikia fusiformes(Hijikia). J Sci Food Agric 76, 56-62   DOI   ScienceOn
26 Yamamoto L, Nagumo T, Takahashi M, Fujihara M, Suzuki Y and Lizima N. 1981. Antimutagenic effect of seaweeds: Ⅲ. Antitumor effect of an extract from Sagassum. Jap J Exp Med 51, 187- 189
27 Bang JD, Chun SK, Park SI and Choi YJ. 1992. Studies on the biochemical and serological charac- teristics of Edwardsiella tarda isolated from cultured flounder, Paralichthys olivaceus. J Fish Pathol 5, 29-35
28 Mayer AMS and Hamann MT. 2002. Marine pharmacology in 1999: compounds with antibacterial, anticoagulant, antifungal, anthelmintic, anti-inflammatory, antiplatelet, antiprotozoral and antiviral activities affecting the cardiovascular, endocrine, immune and nervous system, and other miscellaneous mechanisms of action. Comp Biochem Physiol Part C 132, 315-339   DOI   ScienceOn
29 Skerget M, Kotnik P, Hadolin M, Hras AR, Simonic M and Knez Z. 2005. Phenols, proanthocyanidins, flavones and flavonols in some plant materials and the antioxidant activities. Food Chem 89, 191-198   DOI   ScienceOn
30 Ko MS, Shin KM and Lee MY. 2002. Effects of Hizikia fusiforme ethanol extract on antioxidative enzymes in ethanol-induced hepatotoxicity of rat liver. J Korean Soc Food Sci Nutr 31, 87-91   DOI   ScienceOn
31 Shim YY, An JH, Cho WD, Chun H, Kim KI, Cho HY and Yang HC. 2002. Inhibitory mechanism of blood coagulation and in vivo anticoagulant activities of polysaccarides isolated from Codium fragile. J Korean Soc Food Sci Nutr 31, 917-923   DOI   ScienceOn
32 Kim SS, Galaz GB and Lee KJ and Lee YD. 2006. Effects of dietary supplementation of Spirulina and Astaxanthin for juvenile olive flounder, Paralichthys olivaceus in low temperature season. J Aquaculture 19, 57-63
33 Clavijo AM, Conray G, Santander J and Aponte F. 2002. First report of E. tarda from tilapias in Venezuela. Bull Eur Assoc Fish Pathol 22, 280-282
34 Hong JH, Son BS, Kim BK, Chee HY, Song KS, Lee BH, Shin HC and Lee KB. 2006. Antihypertensive effect of Ecklonia cava extract. Kor J Pharmacogn 37, 200-205
35 Kim JA and Lee JM. 2004. The changes of biologically functional compounds and antioxidant activities in Ecklonia cava with blanching times. Korean J Food Culture 19, 369-377
36 Sae-Dui D, Muroga K and Nakai T. 1984. A case of Edwardsiella tardain cultured colored carp, Cyprinius carpio. Fish Pathol 19, 197-199   DOI
37 Kim SS and Lee KJ. 2008. Effects of dietary kelp (Ecklonia cava) on growth and innate immunity in juvenile olive flounder Paralichthys olivaceus (Temminck et Schlegel). Aquac res 39, 1687-1690
38 Kim YY, Lee KW, Kim GB and Cho YJ. 2000. Studies on physiochemical and biological properties of depolymerized alginate from sea tangle, Laminaria japonicus by thermal decomposition. J Kor Fish Soc 33, 393-398   DOI   ScienceOn
39 Shiau SY and Yu YP. 1999. Dietary supplementation of chitin and chitosan depresses growth in tilapia, Oreochromis niloticus ${\times}$ O aureus. Aquaculture 179, 439-436   DOI   ScienceOn