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http://dx.doi.org/10.5657/kfas.2008.41.4.243

Biochemical Properties of Hemolysin Produced by Vibrio cholerae non-O1 CT Isolated from Sea Water  

Kim, Young-Mog (Department of Food Science and Technology, Pukyong University)
Lee, Myung-Suk (Department of Microbiology, Pukyong University)
Kim, Young-Man (Department of Food Science and Nutrition, Dongeui University)
Kwon, Hyun-Ju (Department of Life Science and Biotechnology, Dongeui University)
Kim, Byung-Woo (Department of Life Science and Biotechnology, Dongeui University)
Kim, Kwang-Hyeon (Department of Life Science and Biotechnology, Dongeui University)
Yeom, Jong-Hwa (Department of Clinical Laboratory Science, Dongeui University)
Lee, Eun-Woo (Department of Life Science and Biotechnology, Dongeui University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.41, no.4, 2008 , pp. 243-247 More about this Journal
Abstract
The hemolysin of Vibrio cholerae non-O1 CT isolated from sea water was purified and characterized. The purified hemolysin displayed an optimum at $37^{\circ}C$ and exhibited more than 70% of residual hemolytic activity after incubation at $45^{\circ}C$ for 120 min. However, the activity dropped dramatically at temperature above $55^{\circ}C$. The purified protein showed the highest hemolytic activity at pH 7.0, while the activity was completely lost outside of the pH ranges of 5.0 and 10.0. The activity of hemolysin was inactivated by addition of divalent cations, such as $Cu^{2+},\;Fe^{2+},\;Hg^{2+},\;Mn^{2+},\;and\;Zn^{2+}$, however, the activity was not completely inhibited by additions of $Ca^{2+},\;Mg^{2+},\;K^+,\;Na^+,\;and\;Li^+$.
Keywords
Hemolysin; Hemolytic activity; Vibrio cholerae non-O1;
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Times Cited By KSCI : 3  (Citation Analysis)
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