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http://dx.doi.org/10.5657/kfas.2008.41.3.228

Comparison of Panning and Sodium Polytungstate Methods for Separating Dinoflagellate Cysts  

Kang, Yoon-Ja (Department of Oceanography, Pukyong National University)
Moon, Chang-Ho (Department of Oceanography, Pukyong National University)
Cho, Hyun-Jin (Korea Coast Guard)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.41, no.3, 2008 , pp. 228-231 More about this Journal
Abstract
Two methods for separating dinoflagellate cysts from marine sediments were compared: panning and sodium polytungstate (SPT). After pretreatment with the SPT solution, Protoperidinium species had a high germination rate and cell growth rate. The germinated cells grew continuously, even in a low nutrient environment. Alexandrium cysts did not germinate, because their germination is usually active in winter. Using SPT solution, the separation process was easier, quicker, and caused much less eye fatigue than the panning method. Therefore, SPT solution offers a convenient way of pretreating dinoflagellate cysts for separation and analysis.
Keywords
Dinoflagellate cysts; Germination; SPT solution method; Panning method;
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