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http://dx.doi.org/10.5657/kfas.2006.39.spc1.123

Flow Cytometric Assessment of Immune Parameters of the Manila Clam (Ruditapes philippinarum)  

Park Kyung-Il (School of Applied Marine Science, Cheju National University)
Park Heung-Sik (Korea Ocean Research and Development Institute (KORDI))
Kim Jong-Man (Korea Ocean Research and Development Institute (KORDI))
Park Young-Je (National Fisheries Development and Research Institute (NFRDI))
Hong Jae-Sang (Department of Oceanography, Inha University)
Choi Kwang-Sik (School of Applied Marine Science, Cheju National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.39, no.spc1, 2006 , pp. 123-131 More about this Journal
Abstract
Immmune parameters of the Manila clam Ruditapes philippinarum collected from four tidal flats, Nudong, Gonam, Hwangdo and Bangpo on Anmyeon-do, Korea were optimized and evaluated at the single cell level using flow-cytometry Hemocytes were withdrawn from the sinus of each clam, and total hemocyte counts (THC), phagocytosis rate, hemocyte mortality (HM) and DNA damage of hemocyte were analyzed. The highest hemocyte counts was recorded from the clams collected from Gonam, followed by Hwangdo, Nudong and Bangpo (P<0.001). Phagocytosis rate and hemocyte mortality of Gonam and Nudong clams were significantly higher than those of clams from Hwangdo and Bangpo (P<0.001). DNA damage in the clams from Nudong was higher twice than that of clams from Gonam (P<0.05). We suggest that the flow-cytometry has a high potential for evaluation of immunity of marine bivalves.
Keywords
Ruditapes philippinarum; Flow cytometry; Immune parameter; Phagocytosis; Hemocyte; DNA damage;
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