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http://dx.doi.org/10.5657/kfas.2006.39.4.318

Anticancer Activity of Ultrasonified Extracts from Seawater-based Culture of the Microalga Spirulina platensis  

Kim, Hyou-Sung (School of Biotechnology and Bioengineering, Kangwon National University)
Kim, Cheol-Hee (School of Biotechnology and Bioengineering, Kangwon National University)
Kwon, Min-Chul (School of Biotechnology and Bioengineering, Kangwon National University)
Song, Young-Kyu (School of Biotechnology and Bioengineering, Kangwon National University)
Cho, Jung-Hwan (Kum-young food)
Gwak, Hyeong-Geun (SKY007)
Hwang, Bo-Young (SKY007)
Kim, Jin-Chul (School of Biotechnology and Bioengineering, Kangwon National University)
Lee, Hyeon-Yong (School of Biotechnology and Bioengineering, Kangwon National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.39, no.4, 2006 , pp. 318-325 More about this Journal
Abstract
Ultrasonified extracts from seawater-based cultures of the microalga Spiyulina platensis were obtained using water and ethanol at 60 and 100$^{\circ}C$. The yield of the aqueous fraction of S. platensis extracted using ultrasonification was about 33.46%. The cytotoxicity against HEK293 and inhibition ratios of the cancer cell lines A549, AGS, MCF7, and Hep3B were measured using the sulforhodamine-B (SRB) assay. The cytotoxicity of all extracts at 1.0 mg/mL was below 26%. The cytotoxicity of the ultrasonified extracts from the seawater-based culture of the microalga Spirulina platensis was about 4% less than that of Spirulina platensis without ultrasonification. The inhibition ratio of cancer cell growth was approximately 80% for 1.0 mg/mL extracts. The inhibitory effect on cancer cell growth was greater for seawater containing ultrasonified Spirulina platensis extracts than for extracts without ultrasonification. The differentiation ratio of HL-60 cells was 160.9%. Densitometric analysis of Bcl-2 revealed that the ultrasonified extracts had greater anticancer activity than the extracts without ultrasonification.
Keywords
Spirulina platensis; Cytotoxicity; Anticancer activity; Ultrasonification;
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