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http://dx.doi.org/10.5657/kfas.2005.38.3.148

Purification and Characterization of a Novel Neuvopeptide with a Smooth Muscle-Relaxing Activity from the Starfish, Asterina pectinifera  

KIM Eun Jung (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
KIM Chan-Hee (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
GO Hye-Jin (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
KIM In Hae (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
AN Sang Hyun (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
SOHN Hee-Young (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
PARK Hee Yeon (Biotechnology Research, National Fisheries Research & Development Institute)
YOON Ho Dong (Biotechnology Research, National Fisheries Research & Development Institute)
CHANG Young-Chae (Department of Pathology, School of Medicine, Catholic University of Daegu)
HONG Yong-Ki (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
PARK Nam Gyu (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.38, no.3, 2005 , pp. 148-152 More about this Journal
Abstract
A novel neuropeptide with a relaxing activity on the dorsal retractor muscle (DRM) was isolated from the whole body extract of the starfish, Asterina pectinifera. The peptide was purified by gel-filtration ion-exchange and $C_{18}$ reversed-phase HPLC. The complete amino acid sequence of this peptide, which was determined by automated Edman degradation and MALDI-TOF mass, was Phe-Gly-Lys-Gly-Gly-Ala-Tyr-Asp-Pro-Leu-Ser-Ala-Gly-Phe-Thr-Asp. A comparison of the amino acid sequence with those of other known neuropeptides revealed that the asteripectin was a novel neuropeptide with smooth muscle-relaxing activity on the starfish DRM. This peptide showed threshold response to relaxing activity on the DRM at $10^{-10}M$ and the maximal relaxing effect was $120{\pn}7.0\%$ at $10^{-5}M$. The relaxing activity of this peptide on the starfish DRM increased in a dose-dependent manner.
Keywords
Asterina pectinifera; Dorsal retractor muscle; Relaxing activity; Starfish;
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