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http://dx.doi.org/10.5657/kfas.2005.38.3.143

Structure and Arterial Relaxing Activity of Substance P-related Peptides and Substance P analogs  

KIM Eun Jung (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
KIM Chan-Hee (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
GO Hye-Jin (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
KIM In Hae (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
AN Sang Hyun (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
SOHN Hee-Young (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
PARK Hee Yeon (Biotechnology Research, National Fisheries Research & Development Institute)
YOON Ho Dong (Biotechnology Research, National Fisheries Research & Development Institute)
CHANG Young-Chae (Department of Pathology, School of Medicine, Catholic University of Daegu)
HONG Yong-Ki (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
PARK Nam Gyu (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.38, no.3, 2005 , pp. 143-147 More about this Journal
Abstract
Relationship between structure and biological activity was performed using the five substance P-related peptides (SPRPs) [mammalian-SP (M-SP), cod-SP (C-SP), trout-SP (T-SP), lungfish-SP (L-SP) and Ranakinin] and four SP analogs [[$His^5$]-SP (5H-SP), [$Gly^5$]-SP (5G-SP), ($Ile^8$)-SP (8I-SP) and ($Trp^8$)-Ranakinin (8W-Ranakinin)]. The circular dichroism (CD) spectra showed that all of the peptides took an unordered structure in buffer solution and artificial liposomes. However, 8W-Ranakinin undergoes conformational changes by being transferred into neutral and acidic liposomes from an unordered structure to more ordered structure. The arterial relaxing effect of the peptides was also studied with guinea-pig aorta (GPA), As a result of the studies, L-SP was about 14-fold more potant than M-SP. The order of potency compared to $EC_{50}$ value was $L-SP{\gg}M-SP>5G-SP{\ge}8I-SP>5H-SP>T-SP$, C-SP, Ranakinin, 8W-Ranakinin.
Keywords
CD spectra; Guinea-pig aorta; Retaking activity; Substance P; Peptide;
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