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http://dx.doi.org/10.5657/kfas.2004.37.1.057

Effects of Light and Temperature on Nitrate Uptake, Germling Growth and Fatty Acid Composition of Enteromorpha compressa (Chlorophyta)  

LEE Dong Hoon (Department of Marine Biology, Pukyong National University)
LEE Soon Jeong (Department of Marine Biology, Pukyong National University)
RYU Jina (Department of Marine Biology, Pukyong National University)
PARK Eunjeong (Department of Marine Biology, Pukyong National University)
NAM Ki Wan (Department of Marine Biology, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.37, no.1, 2004 , pp. 57-64 More about this Journal
Abstract
Effects of light and temperature on the nitrate uptake and germling growth of Enteromorpha compressa (L.) Greville (Chlorophyta) were studied based on samples from Cheongsapo near Busan, Korea. In addition, their effects on fatty acids composition in thallus were examined. Nitrate uptake showed saturation kinetics. $V_{max}$ (maximal uptake rate) and its $K_s$ (half-saturation constant) at $20^{\circ}C,\;80\;{\mu}mol\;m^{-2}s^{-1},$ white light were $1.571\;{\mu}mol{\cdot}g\;fr\;wt^{-1}{\cdot}h^{-1}$ and 3.56 ${\mu}M$, respectively. In nitrate uptake with irradiance, wavelength and temperature, its rate represented respectively the highest value as $1.405\pm0.020,\;0.623\pm0.040,\;1.422\pm0.022\;{\mu}mol{\cdot}g\;fr\;wt^{-1}{\cdot}h^{-1}\;at\;100\;{\mu}mol\;m^{-2}s^{-1},$ red light, $20^{\circ}C$ and exhibited significant difference among the examined conditions (p<0.001). Germling growth of E. compressa also showed saturation kinetics, and $V_{max}$ and its $K_s$ value at $20^{\circ}C,\;100\;{\mu}mol\;m^{-2}s^{-1},$ 12:12 h were $56.18\%\;day^{-1}$ and 0.33 ${\mu}M$, respectively. SGR (specific growth rate) recorded a maximal value as 49.33-54.80, 39.07-50.72, $47.20-54.53\%\;dat^{-1}$ at $120\;{\mu}mol\;m^{-2}s^{-1},$ blue light and $18^{\circ}C$ respectively, and showed significant difference (p<0.001). Red light made the effective nitrate uptake, but germling growth was largely limited by the light. In fatty acids analysis, PUFAs (polyunsaturated fatty acids) were high at blue light, $18^{\circ}C,\;100\;{\mu}M\;NO_3^-.$ However, irradiance did not affect the production of PUFAs. In conclusion, nitrate uptake and germling growth of E. compressa showed saturation kinetics to external nitrate concentration, and were significantly affected by irradiance, wave length and temperature. Fatty acid composition was also influenced by the factors except for irradiance. Their maximal values, together with the highest production of PUFAs, were found at blue light band, $20^{\circ}C,\;100\;{\mu}mol\;m^{-2}s^{-1},\;and\;100\;{\mu}M\;NO_3^-.$
Keywords
Enteromorpha compressa; Nitrate uptake; Germling growth; Fatty acid composition; Temperature;
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1 Probyn, T.A and A.R.O. Chapman. 1982. Nitrogen uptake characteristics of Chordaria flagelliformis (Phaeo- phyta) in batch mode and continuous mode experi-ments. Mar. Biol., 71, 129-133   DOI
2 Provasoli, L. 1968. Media and prospects for the cultivation of marine algae. In: Culture and Collections of Algae, Watanabe, A. and A. Hattori, eds. Jap. Soc. Plant Physiol., Japan, pp. 63-75
3 Raven, J.A. 1974. Carbon dioxide fixation. In: Algal Physiology and Biochemistry, Stewart, W.D.P. ed. Blackwell Scientific Publications, Oxford, pp. 434- 455
4 Round, F.E. 1981. The Ecology of Algae. Cambridge University Press, Cambridge, pp. 653
5 Ryther, J.H. and W.M. Dunstan. 1971. Nitrogen, pho-sphorus and eutrophication in the coastal marine environment. Science, 171, 1008-1013   DOI   ScienceOn
6 Lobban, C.S. and P.J. Harrison. 1994. Seaweed Ecology and Physiology. Cambridge University Press, New York, pp. 336
7 Lopez-Figueroa, F. and W. Rudiger. 1991. Stimulation of nitrate net uptake and reduction by red and blue light and reversion by far-red light in the green alga Ulva rigida. J. Phycol., 27, 389-394   DOI
8 McGlathery, K.J., M.F. Pedersen and J. Borum. 1996. Changes in intracellular nitrogen pools and feedback controls on nitrogen uptake in Chaetomorpha linum (Chlorophyta). J. Phycol., 32, 393-401   DOI   ScienceOn
9 Morgan, K.C. and F.J. Simpson. 1981. Cultivation of Palmaria palmata (Rhodymeniales): effect of high concentrations of nitrate and ammonium on growth and nitrogen uptake. Aquat. Bot., 11, 167-171   DOI   ScienceOn
10 Ohno, M. 1969. A physiological ecology of the early stage of some marine algae. Rep. USA Mar. Biol. Stn., 16, 1-46
11 Fern$\ndez-Reiriz, M.J., A. Perez-Camacho, M.J. Ferreiro, J. Blanco, M. Planas, M.J. Campos and U. Labarta. 1989. Biomass production and variation in the bio- chemical profile (Total protein, carbohydrates, RNA, lipids and fatty acids) of seven species of marine microalgae. Aquaculture, 83, 17-37   DOI   ScienceOn
12 Floreto, E.A.T., H. Hirata., S. Ando and S. Yamasaki. 1993. Effects of temperature, light intensity, salinity and source of nitrogen on the growth, total lipid and fatty acid composition of Ulva pertusa Kjellman (Chlorophyta). Bot. Mar., 36, 149-158   DOI   ScienceOn
13 Fujita, R.M., P.A. Wheeler and R.L. Edwards. 1989. Assessment of macroalgal nitrogen limitation in a seasonal upwelling region. Mar. Ecol. Prog. Ser., 53, 293-303   DOI
14 De Boer, J.A. 1981. Nutrients. In: The Biology of Sea- weeds, Lobban, C.S. and M.J. Wynne, ed. Blackwell Scientific Publications, Oxford, pp. 356-391
15 Choi, C.G., H.G. Kim and B.O. Jun. 1994. On the nitrate uptake in Ulva pertusa Kjellman. Kor. J. Phycol., 9, 247-253
16 Dayton, P.K. 1971. Competition, disturbance and com- munity organization: the provision and subsequent utilization of space in a rocky intertidal community. Ecol. Monogr., 41, 351-389   DOI   ScienceOn
17 De Boer, J.A., H.J. Guigli and C.F. D'Elia. 1978. Nutritional studies of two algae. I. Growth rate as a function of nitrogen source and concentration. J. Phycol., 14, 261-266   DOI
18 Del Río, M.J., Z. Ramazanov and G. Garcia-Reina. 1996. Ulva rigida (Ulvales: Chlorophyta) tank culture as biofilters for dissolved inorganic nitrogen from fishpond effluents. Hydrobiologia, 326, 61-66   DOI
19 Dring, M.J. 1986. Pigment composition and photosynthetic action spectra of sporophytes of Laminaria (Phaeo- phyta) growth in different light quality and irra- diances. Br. Phycol. J., 21, 199-207   DOI   ScienceOn
20 Wheeler, W.N. 1982. Nitrogen nutrition of Macrocystis. In: Synthetic and Degradative Processes in Marine Macrophytes, Srivastava, L.M. ed. Walter De Gruyter, Berlin, pp. 317
21 Wheeler, P.A. and W.J. North. 1980. Effect of nitrogen supply on nitrogen content and growth rate of juwthile Macrocystis pyrifera (Phaeophyta) sporophytes. J. Phycol., 16, 577-582   DOI
22 Zar, J.H. 1999. Multi-way factorial analysis of variance. In: Biostatistical Analysis, 4th ed., Prentice-Hall International, Inc., London, pp. 282-302
23 Syrett, P.J. 1981. Nitrogen metabolism of microalgae. In: Physiological Base of Phytoplankton Ecology, Vol. 210, T. Platt, ed. Can. Bull. Fish. Aquat. Sci., pp. 182-210
24 Sasakawa, H. and Y. Yamamoto. 1979. Effects of red, far-red and blue light on enhancement of nitrate reductase activity and on uptake in etiolated rice seedlings. Plant Physiol., 63, 1098-1101   DOI   ScienceOn
25 Senger, H. 1980. The Blue Light Syndrome. Springer- Verlag, New York, pp. 665
26 Sokal, R.R. and F.J. Rohlf. 1994. Nested analysis of variance. In: Biometry, 3rd ed., Freeman, W.H. and Company, San Francisco, pp. 272-342
27 Tenore, K.R. 1976. Food chain dynamics of abalone polyculture system. Aquaculture, 8, 23-27   DOI   ScienceOn
28 Thomas, T.E and P.J. Harrison. 1987. Rapid ammonium uptake and nitrogen interactions in five intertidal seaweeds grown under field conditions. J. Exp. Mar. Biol. Ecol., 107, 1-8   DOI   ScienceOn
29 Kim, K.Y., Y.S. Ahn and I.K. Lee. 1991. Growth and morphology of Enteromorpha linza (L.) J. Ag. and E. prolifera (Muller) J. Ag. (Ulvales, Chlorophyceae). Kor. J. Phycol., 6, 31-35
30 Kim, K.Y and I.K. Lee. 1993. Combined effects of irradiance-salinity and temperature-salinity on the growth of Enteromorpha compressa (Chlorophyta) in laboratory culture. Kor. J. Bot., 36, 219-224
31 Lapointe, B.E. and K.R. Tenore. 1981. Experimental out-door studies with Ulva fasciata Delile. I. Interactions of light and nitrogen on nutrient uptake, growth and biochemical composition. J. Exp. Mar. Biol. Ecol., 53, 135-152   DOI   ScienceOn
32 Lobban, C.S., P.J. Harrison and M.J. Duncan. 1985. The Physiological Ecology of Seaweeds. Cambridge Uni- versity Press, Cambridge, pp. 31-242
33 Hein, M., M.F. Pedersen and K. Sand-Jensen. 1995. Size-dependent nitrogen uptake in micro-and macro-algae. Mar. Ecol. Prog. Ser., 118, 247-253   DOI
34 Gao, Y., G.J. Smith and R.S. Alberte. 2000. Temperature dependence of nitrate reductase activity in marine phytoplankton: biochemical analysis and ecological implications. J. Phycol., 36, 304-313   DOI   ScienceOn
35 Gomez Pinchetti, J.L., E. del Campo Fernandez., P. Moreno Diez and G.G. Reina. 1998. Nitrogen availability influences the biochemical composition and photosynthesis of tank-cultivated Ulva rigida (Chlorophyta). J. Appl. Phycol., 10, 383-389   DOI   ScienceOn
36 Harlin, M.M. and P.A. Wheeler. 1985. Nitrate uptake. In: Handbook of Phycological Methods. Ecological Field Methods: Macroalgae, Littler, M.M. and D.S. Lettler, ed. Cambridge University Press, Cambridge, pp. 493-508
37 Jones, W.E. and E.S. Dent. 1970. The effect of light on the growth of algal spores. Helgoländer wiss. Mee- resunters, 20, 70-78   DOI
38 Bligh, E.G. and W.J. Dyer. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol., 37, 911-917   DOI
39 Callow, J.A and M.E. Callow. 1997. Primary adhesion of Enteromorpha (Cholorophyta, Ulvales) propagules: quantitative settlement studies and video microscopy. J. Phycol., 33, 938-947   DOI   ScienceOn
40 Chapman, A.R.O., J.W. Markham and K. Lüning. 1978. Effects of nitrate concentration on the growth and physiology of Laminaria saccharina (Phaeophyta) in culture. J. Phycol., 14, 195-198   DOI
41 Azuara, M.P. and P.J. Aparicio. 1983. In vivo blue-light activation of Chlamydomonas reinhardii nitrate re- ductase. Plant Physiol., 71, 286-290   DOI   ScienceOn
42 Ahlgren, G., I.B. Gustafsson and M. Boberg. 1992. Fatty acid content and chemical composition of freshwater microalgae. J. Phycol., 28, 37-50   DOI
43 Altamirano, M., C. Francisco and F.L. Figueroa. 2000. Growth seasonality, photosynthetic pigments, and carbon and nitrogen content in relation to environ- mental factors: a field study of Ulva olivascens (Ulvales, Chlorophyta). Phycologia, 39, 50-58   DOI   ScienceOn
44 APHA AWWA WPCF. 1985. Standard Methods for Examination of Water and Wastewater. American Public Health Association, Washington, DC, pp. 396