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http://dx.doi.org/10.5657/kfas.2002.35.6.676

Molecular Cloning and Alternative Splicing of Growth Hormone Transcripts in Greenling, Hexagrammos otakii  

Nam Yoon Kwon (Fish Cenetic Manipulation Laboratory, Department of Aquaculture, Pukyong National University)
Kim Dong Soo (Fish Cenetic Manipulation Laboratory, Department of Aquaculture, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.35, no.6, 2002 , pp. 676-681 More about this Journal
Abstract
Different types of transcripts encoding growth hormone (GH) were identified from cDNA libraries constructed with pituitaries of a marine fish species, greenling (Hexagrammos otakii). GH-homologous cDNA clones were isolated using the high-density filter hybridization and the expressed sequence tag techniques. Of 39 full-length positive cDNA clones, 31 clones ($79\%$) displayed an identical sequence, however, remaining 8 clones exhibited several polymorphisms in their sequences including (1) the length and sequence variability in the 5' upstream region, (2) insertional sequences in open reading frame, and (3) deletion and/or single nucleotide polymorphism in the untranslated 3' region. Based on RT-PCT and RNA dot blot analyses, these transcripts were proven to be expressed in a pituitary-specific manner.
Keywords
Hexagranmnos otakii; GH; cDN4 Sequence variation;
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1 Gallo, A., E. Thomson, J. Brindle, M.A. O'Connell and L.P. Keegan. 2002. Micro precessing events in mRNAs identified by DHPLC analysis. Nucleic Acids Res., 30, 3945-3953   DOI   ScienceOn
2 Graveley, B.R. 2001. Alternative splicing: increasing diversity in the proteomic world. Trends Genet., 17, 100-107   DOI   ScienceOn
3 Hinits, Y. and B. Moav. 1999. Growth performance studies in transgenic Cyprinus carpio. Aquaculture, 173, 285-296   DOI   ScienceOn
4 Nam, Y.K. and D.S. Kim. 2001. Bioinformatics in fish: its present status and perspectives with particular emphasis on expressed sequence tags - brief review. J. Aquacult, 14, 9-16
5 Agellon, L.B. and T.T. Chen. 1986. Rainbow trout growth hormone: molecular cloning of cDNA and expression in Escherichia coli. DNA, 5, 463-471   DOI   ScienceOn
6 Ber, R. and V. Daniel. 1993. Sequence analysis suggests a recent duplication of the growth honnone-encoding gene in Tilapia nilotica. Gene, 125, 143-150   DOI   ScienceOn
7 Brett, D., J. Hanke, G. Lehmann, S. Haase, S. Delbruck, J. Reich and P. Bork. 2000. EST comparison indicates 38% of human mRNA contain possible alternative splice forms. FEBS Lett., 474, 83-86   DOI   ScienceOn
8 Chiou, C.-S., H.-T. Chen and W.-C. Chang. 1990. The complete nucleotide sequence of the growth-hormone gene from the common carp (Cyprinus carpio). Biochim. Biophy. Acta, 1087, 91-94   DOI   ScienceOn
9 Nam, Y.K., Y.S. Cho, S.E. Douglas, J.W. Gallant, M.E. Reith and D.S. Kim. 2002. Isolation and transient expression of a cDNA encoding L-gulono-g-lactone oxidase, a key enzyme for L-ascorbic acid biosynthesis, from the tiger shark Scyliorhinus torazame. Aquaculture, 209, 271-284   DOI   ScienceOn
10 Nam, Y.K., J.K. Noh, Y.S. Cho, H.J. Cho, K.N. Cho, C.G. Kim and D.S. Kim. 2001. Dramatically accelerated growth and extraordinary gigantism of transgenic mud loach (Misgurnus mizolepis). Transgenic Res., 10, 353-362   DOI   ScienceOn
11 Shen, H., H. Illges, A. Reuter and C.A.O. Stuenner. 2002. Cloning, expression, and alternative splicing of neogenin 1 in zebrafish. Mech. Dev., 118, 219-223   DOI   ScienceOn
12 Young, G., B.T. Bjornsson, P. Prunet, R.J. Lin and H.A. Bern. 1989. Smoltification and seawater adaptation in coho salmon (Oncorhynchus kisutch): plasma prolactin, growth hormone, thyroid hormones, and cortisol. Gene. Comp. Endocrinol., 74, 335-345   DOI   ScienceOn
13 Dunham, R.A. and R.H. Devlin. 1999. Comparison of traditional breeding and transgenesis in farmed fish with implications for growth enhancement and fitness. In: Transgenic Animals in Agriculture: Murray J.D., G.B. Anderson, A.M. Oberbauer and M.N. McGloughlin (eds). pp. 209-229. CAB International, Wallingford, UK
14 Untergasser, G., M. Hermann, H. Rumpold, G. Pfister and P. Berger. 2000. An unusual member of the human growth hormone/placental lactogen (GH/PL) family, the testicular altemative splicing variant hPL-A2: recombinant expression revealed a membrane-associated growth factor molecule. Mol. Cell. Endocnnol., 167, 117-125   DOI   ScienceOn
15 Devlin, R.H., T.Y. Yesaki, E.M. Donaldson, S.J. Du and C.L. Hew. 1995. Production of germline transgenic Pacific salmonids with dramatically increased growth performance. Can. J. Fish. Aquat. Sci., 52, 1376-1384   DOI
16 Douglas, S.E., J.W. Gallant, C.E. Bullerwell, C. Wolff, J. Munholland and M.E. Reith. 1999. Winter flounder expressed sequence tags: establishment of an EST database and identification of novel fish genes. Mar. Biotechnol., 1, 458-464   DOI   ScienceOn
17 Forbes, S.H., K.L. Knudsen, T.W. North and F.W. Allendorf. 1994. One of two growth hormone genes in coho salmon is sex-linked. Proc. Natl. Acad. Sci. USA, 91, 1628-1631   DOI   ScienceOn