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http://dx.doi.org/10.5657/kfas.2002.35.6.595

Comparison of Blood Physiology in Juvenile Black Seabream (Acanthopagrus schlegeli) Reared in Converted Freshwater from Seawater and Seawater from Freshwater  

Chang Young Jin (Department of Aquaculture, Pukyong National Univerity)
Min Byung Hwa (Department of Aquaculture, Pukyong National Univerity)
Chang Hae Jin (Department of Aquaculture, Pukyong National Univerity)
Hur Jun Wook (Department of Aquaculture, Pukyong National Univerity)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.35, no.6, 2002 , pp. 595-600 More about this Journal
Abstract
Comparison of blood properties in juvenile black seabream (Acanthopans schlegeli) between transfer from seawater to freshwater (sFW) and transfer from freshwater to seawater (fSW) were investigated for 60 days. Plasma cortisol levels in sFW were significantly increased from 34.2 $\pm$ 28.6 ng/mL at the beginning to 365.6 $\pm$ 136.0 ng/mL at 3 hours, and decreased to the beginning level at 24 hours. However, in fSW, no significant differences in the cortisol levels were recognized throughout experimental period. No significant difference was found in the glucose levels between sFW and fSW. The transfer from seawater to freshwater of juvenile black seabream resulted in reduced $Na^{+}\;and\; Cl^{-}$ concentrations for 24 hours (decreasing from 165.3 $\pm$ 2.5, 146.0$\pm$ 2.2 to 118.3 $\pm$ 12.3, 78.0$\pm$ 7.0 mEq/L, respectively), but these were completely recovered the beginning levels at 30 days. Total protein and AST showed no significant differences between the two rearing conditions, while ALT was markedly elevated at 3 hour in sFW and at 24 hours in fSW. Ht in sFW was increased from 18.5 $\pm$ $0.6\%$ at the beginning to 25.3 $\pm$ $4.0\%$ at 12 hours, and was decreased to the beginning level at 24 hours. Ht, RBC and Hb in fSW were also significantly higher at 12 hours, but recovered to their initial levels at 24 hours. All fish were dead until 50 days in sFW while survival rate in fSW was $85\%$ at the end of experiment.
Keywords
Black seabream; Acanthopagus schlegeli; Freshwater acclimation; Salinity change; Blood physiology; Stress; Survival;
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