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http://dx.doi.org/10.5657/kfas.2002.35.5.524

Glycosyl-linkages of Acid Soluble Polysaccharide from Green Laver, Enteromopha prolifera  

Koo Jae Geun (Department of Food Science and Technology, Kunsan National University)
Choi Yong Seok (Department of Food Science and Technology, Kunsan National University)
Ha Jin Hwan (Department of Food Science and Technology, Cheju National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.35, no.5, 2002 , pp. 524-528 More about this Journal
Abstract
Green layer, Enteromorpha prolifera, is regarded as one of important materials for food processing in Korea. The acidic water-soluble polysaccharide (CPC-PS) isolated from the alga with hot water and cetylpyridium chloride was mainly constituted of rhamnose, xylose, uronic acid and sulfate. To determine the glycosyl-linkages and positions of sulfate by methylation, the CPC-PS was reduced and/or sulfates. A marked increase of glucose content in the reduced polysaccharide indicated that glucuronic acid was a major sugar in the polymer and sulfation was deduced to occur on O-3 of rhamnose and O-2 of xylose. According to the methylation analysis of the native, reduced, desulfated and reduced-desulfated polymers, CPC-PS mainly composed of 1,4- and 1,2,3-linked rhamnose 3-sulfate, 1,4-linked xylose 2-sulfate, 1,4-linked xylose and 1,4-linked glucuronic acid. Minor 1,4-linked rhamnose and 1,4,6-linked galactose residues were also detected.
Keywords
Green laver; Methylation; Enteromorpha prolifera; Glycosyl-linkage; Acidic water-soluble polysaccharide;
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