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http://dx.doi.org/10.4163/jnh.2020.53.2.121

Comparison of whitening effect of Rubus coreanus fruit according to maturity  

Park, Jeong-Yong (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Lee, Ji Yeon (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Seo, Kyung Hye (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Jang, Gwi Young (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Lee, Seung Eun (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Ji, Yun-Jeong (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Kim, Hyung Don (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Publication Information
Journal of Nutrition and Health / v.53, no.2, 2020 , pp. 121-128 More about this Journal
Abstract
Purpose: The Rubus coreanus fruit (RF) is an important traditional medicinal herb having antioxidant, anti-inflammatory, and immunoregulatory properties. These activities are known to change dramatically, depending on maturity of the RF. It is presumed that change of functional components, such as flavonoids, tannins, phenolic acids, triterpenoids and organic acids in RF, affect the various bioactivities. This study aimed to confirm changes in the anti-melanogenic effects of RF based on maturity, and to identify the bioactive compounds responsible. Methods: The cell viability of mature RF (MRF) and immature RF (IRF) extracts was investigated using B16F10 cells. To compare the anti-melanogenic effect of MRF and IRF extracts, we first assessed the melanin content. High-performance liquid chromatography analysis was performed to evaluate changes in the level of ellagic acid according to maturity of the RF. In addition, tyrosinase inhibitory activity of both extracts was examined. Results: MRF and IRF extracts (50-200 ㎍/mL) do not affect the cell viability of B16F10 melanoma cells. IRF extract more effectively inhibited melanin synthesis than MRF extract. The content of ellagic acid in IRF extract was higher than that obtained in MRF extract. Furthermore, greater inhibition of tyrosinase activity was observed after exposure to IRF extract than MRF extract. A positive correlation was determined between ellagic acid content and tyrosinase inhibitory activity, and a negative correlation was obtained between ellagic acid content and melanin content. Taken together, our results indicate that ellagic acid is one of the major bioactive compounds of RF that imparts a whitening effect. Conclusion: Our results indicate that ellagic acid in MRF and IRF extracts affect the anti-melanogenesis effect through inhibition of tyrosinase activity. Therefore, the ellagic acid rich IRF has greater potential for application as a natural and functional cosmetic material.
Keywords
Rubus coreanus; ellagic acid; melanoma cell; tyrosinase; melanin;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Park JY, Lee JY, Lee DY, Kim HD, Kim GS, Lee SE, et al. Melanogenesis inhibitory effect of steamed Platycodon grandiflorum on B16F10 cells. Asian J Beauty Cosmetol 2019; 17(1): 37-46.   DOI
2 Jeon S, Hwang W, Hong Y, Kim M, Ahn E, Park S. Inhibitory effects of Hericium erinaceus extracts on melanin synthesis and oxidative stress. Asian J Beauty Cosmetol 2016; 14(4): 427-435.   DOI
3 Videira IF, Moura DF, Magina S. Mechanisms regulating melanogenesis. An Bras Dermatol 2013; 88(1): 76-83.   DOI
4 Heitz MP, Rupp JW, Horn KW. Biocatalytic activity of mushroom tyrosinase in ionic liquids: specific ion effects and the Hofmeister series. Insights Enzym Res 2018; 2(1): 1.
5 Oh TI, Yun JM, Park EJ, Kim YS, Lee YM, Lim JH. Plumbagin suppresses $\alpha$-MSH-induced melanogenesis in B16F10 mouse melanoma cells by inhibiting tyrosinase activity. Int J Mol Sci 2017; 18(2): E320.
6 Yamakoshi J, Otsuka F, Sano A, Tokutake S, Saito M, Kikuchi M, et al. Lightening effect on ultraviolet-induced pigmentation of guinea pig skin by oral administration of a proanthocyanidin-rich extract from grape seeds. Pigment Cell Res 2003; 16(6): 629-638.   DOI
7 Lee SM, You Y, Kim K, Park J, Jeong C, Jhon DY, et al. Antioxidant activities of native Gwangyang Rubus coreanus Miq. J Korean Soc Food Sci Nutr 2012; 41(3): 327-332.   DOI
8 Jeong HS, Han JG, Han JH, Kim Y, Oh SH, Kim SS, et al. Antioxidant activities and skin-whitening effects of nano-encapsuled water extract from Rubus coreanus Miquel. Korean J Med Crop Sci 2009; 17(2): 83-89.
9 Park T, Yang K, Chang S, Kim SY. Anti-inflammatory effect of Rubus coreanus Miquel extract from Jeju Island in LPS-stimulated RAW 264.7 cells. KSBB J 2019; 34(3): 167-172.   DOI
10 Han HM. Inhibitory effects of Terminalia chebula, Sanguisorba officinalis, Rubus coreanus and Rheum palmatum on hepatitis B virus replication in HepG2 2.2. 15 cells. Yakhak Hoeji 1999; 43(4): 458-463.
11 Seo KH, Lee JY, Park JY, Jang GY, Kim HD, Lee YS, et al. Differences in anti-inflammatory effect of immature and mature of Rubus coreanus fruits on LPS-induced RAW 264.7 macrophages via NF-${\kappa}B$ signal pathways. BMC Complement Altern Med 2019; 19(1): 89.   DOI
12 Kim LS, Youn SH, Kim JY. Comparative study on antioxidant effects of extracts from Rubus coreanus and Rubus occidentalis. J Korean Soc Food Sci Nutr 2014; 43(9): 1357-1362.   DOI
13 Han SB, Kwon SS, Kong BJ, Kim KJ, Park SN. Antioxidative effect and tyrosinase inhibitory activity of the unripened fruit extract of Rubus coreanus Miquel. J Soc Cosmet Sci Korea 2013; 39(4): 295-302.   DOI
14 Kim GS, Lee DY, Lee SE, Noh HJ, Choi JH, Park CG, et al. Evaluation on extraction conditions and HPLC analysis method for bioactive compounds of Astragali radix. Korean J Med Crop Sci 2013; 21(6): 486-492.   DOI
15 Hosoi J, Abe E, Suda T, Kuroki T. Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res 1985; 45(4): 1474-1478.
16 Chae KS, Son RH, Park SY, Kim KA, Lee TB, Kwon JW. Analytical method validation of ellagic acid as a marker compound for the standardization of black raspberry extract as a functional ingredient. Food Eng Prog 2014; 18(4): 355-358.   DOI
17 Rivas M, Rojas E, Araya MC, Calaf GM. Ultraviolet light exposure, skin cancer risk and vitamin D production. Oncol Lett 2015; 10(4): 2259-2264.   DOI
18 Mun YJ, Kim J, Lim NY, Lee SY, Seop G, Hwang CY, et al. Inhibitory effect on melanogenesis of Radix glycyrrhizae water extract. J Physiol Pathol Korean Med 2002; 16(6): 1230-1235.
19 Svendby TM, Hansen GH, Backlund A, Dahlback A. Monitoring of the atmospheric ozone layer and natural ultraviolet radiation. Annual report 2018. Kjeller: Norwegian Institute for Air Research; 2019.
20 Yoon Y, Bae S, An S, Choe YB, Ahn KJ, An IS. Effects of ultraviolet radiation on the skin and skin cell signaling pathways. Korean J Aesthet Cosmetol 2013; 11(3): 417-426.
21 Chen H, Zuo Y, Deng Y. Separation and determination of flavonoids and other phenolic compounds in cranberry juice by high-performance liquid chromatography. J Chromatogr A 2001; 913(1-2): 387-395.   DOI
22 Jang TW, Park JH. Antioxidative activities and whitening effects of ethyl acetate fractions from the immature seeds of Abeliophyllum distichum. J Life Sci 2017; 27(5): 536-544.   DOI
23 Vattem D, Shetty K. Biological functionality of ellagic acid: a review. J Food Biochem 2005; 29(3): 234-266.   DOI
24 Yang HM, Lim SS, Lee YS, Shin HK, Oh YS, Kim JK. Comparison of the anti-inflammatory effects of the extracts from Rubus coreanus and Rubus occidentalis. Korean J Food Sci Technol 2007; 39(3): 342-347.
25 Ortiz-Ruiz CV, Berna J, Tudela J, Varon R, Garcia-Canovas F. Action of ellagic acid on the melanin biosynthesis pathway. J Dermatol Sci 2016; 82(2): 115-122.   DOI