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http://dx.doi.org/10.4163/jnh.2019.52.3.250

Anti-oxidant and anti-adipocyte differentiation of Aster glehni and Aster yomena  

Lee, Ji Yeon (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science)
Park, Jeong-Yong (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science)
Kim, Hyung Don (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science)
Lee, Seung Eun (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science)
Lee, Jeong Hoon (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science)
Lee, Yunji (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science)
Seo, Kyung Hye (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science)
Publication Information
Journal of Nutrition and Health / v.52, no.3, 2019 , pp. 250-257 More about this Journal
Abstract
Purpose: Aster glehnii (AG) and Aster yomena (AY) are medicinal plants that belong to the family Compositea and grow widely in Korea. Plants in the genus Aster have been used to treat snakebite wounds or bruises in oriental medicine. This study compared the effects of anti-oxidants and anti-adipocyte differentiation according to the species (the aerial parts of AG and AY). Methods: AG and AY were extracted using 70% ethanol (-E) and water (-W) at room temperature. The anti-oxidant activities were measured by total phenol contents (TPC), total flavonoid contents (TFC), DPPH and $ABTS^+$ assay. In addition, correlation analysis was performed for the anti-oxidant compounds and effect. The level of anti-adipocyte differentiation was assessed using an oil red O assay on pre-adipocytes. Results: AG-W showed higher TPC ($6.92{\mu}g/mL$) and AG-E presented higher TFC ($8.22{\mu}g/mL$) than the other extracts. Furthermore, AG-E exhibited higher radical scavenging activity in the DPPH and $ABTS^+$ assay ($IC_{50}$: 104.88 and $30.06{\mu}g/mL$). In the cytotoxicity assay, AG and AY extracts at concentrations less than $100{\mu}g/mL$ were non toxic. AG-W reduced the lipid accumulation of 3T3-L1 cells significantly after differentiation (70.49%) compared to the other extracts. Conclusion: These results show that the water extract of AG has anti-oxidant effects and reduces the differentiation of 3T3-L1 cells. Therefore, AG has utility as a functional food material for its anti-oxidant activities and ability to prevent lipid accumulation.
Keywords
anti-obesity; radical scavenging activity; Aster glehui; adipocyte;
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Times Cited By KSCI : 3  (Citation Analysis)
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