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http://dx.doi.org/10.4163/jnh.2015.48.6.468

Oxidative damage biomarker levels according to Mn-SOD and GST gene polymorphisms in preschool children  

Shin, You-kyung (Department of Food and Nutrition, Kyung Hee University)
Choi, Ji-Won (Department of Food and Nutrition, Kyung Hee University)
Oh, Se-Young (Department of Food and Nutrition, Kyung Hee University)
Chung, Jayong (Department of Food and Nutrition, Kyung Hee University)
Publication Information
Journal of Nutrition and Health / v.48, no.6, 2015 , pp. 468-475 More about this Journal
Abstract
Purpose: Genetic polymorphisms in antioxidant defense and detoxification genes may modulate the levels of oxidative stress biomarkers. Methods: A total of 301 healthy preschool-aged children in the Seoul and Kyung-gi areas were recruited. DNA was extracted from blood for genotyping of manganese superoxide dismutase (Mn-SOD) Val16Ala, glutathione S-transferase (GST) P1 Ile105Val, GSTT1 present/null, and GSTM1 present/null polymorphisms by PCR-restriction fragment length polymorphism or multiplex PCR analyses. In addition to a questionnaire survey, the levels of urinary 8-hydroxyl-2-deoxiguanosine (8-OHdG) and plasma malondialdehyde (MDA) were measured by ELISA. Results: Significantly higher urinary 8-OHdG concentrations were observed in GSTP1 Ile/Val + Val/Val genotype (p = 0.030), and tended to be higher in Mn-SOD Val/Val genotype (p = 0.065). On the other hand, exposure to environmental tobacco smoking (ETS) and interaction between ETS and gene polymorphisms did not significantly influence either urinary 8-OHdG concentrations or serum MDA. Conclusion: Based on our findings, GSTP1 Ile/Val gene polymorphisms might modulate the levels of oxidative stress biomarkers in healthy preschool children.
Keywords
Genetic polymorphism; Mn-superoxide dismutase; glutathione S-transferase; 8-hydroyxyl-2-deoxyguanosine;
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1 Ames BN. Dietary carcinogens and anticarcinogens. Oxygen radicals and degenerative diseases. Science 1983; 221(4617): 1256-1264.   DOI
2 Zhang H, Davies KJ, Forman HJ. Oxidative stress response and Nrf2 signaling in aging. Free Radic Biol Med 2015; 8(Pt B): 314-336.
3 Thanan R, Oikawa S, Hiraku Y, Ohnishi S, Ma N, Pinlaor S, Yongvanit P, Kawanishi S, Murata M. Oxidative stress and its significant roles in neurodegenerative diseases and cancer. Int J Mol Sci 2015; 16(1): 193-217.   DOI
4 Dikalov SI, Ungvari Z. Role of mitochondrial oxidative stress in hypertension. Am J Physiol Heart Circ Physiol 2013; 305(10): H1417-H1427.   DOI
5 Yan MH, Wang X, Zhu X. Mitochondrial defects and oxidative stress in Alzheimer disease and Parkinson disease. Free Radic Biol Med 2013; 62: 90-101.   DOI
6 Zhu H, Li YR. Oxidative stress and redox signaling mechanisms of inflammatory bowel disease: updated experimental and clinical evidence. Exp Biol Med (Maywood) 2012; 237(5): 474-480.   DOI
7 Kim YJ, Kim EH, Hahm KB. Oxidative stress in inflammationbased gastrointestinal tract diseases: challenges and opportunities. J Gastroenterol Hepatol 2012; 27(6): 1004-1010.   DOI
8 Shimoda-Matsubayashi S, Matsumine H, Kobayashi T, Nakagawa- Hattori Y, Shimizu Y, Mizuno Y. Structural dimorphism in the mitochondrial targeting sequence in the human manganese superoxide dismutase gene. A predictive evidence for conformational change to influence mitochondrial transport and a study of allelic association in Parkinson's disease. Biochem Biophys Res Commun 1996; 226(2): 561-565.   DOI
9 Klaunig JE, Kamendulis LM, Hocevar BA. Oxidative stress and oxidative damage in carcinogenesis. Toxicol Pathol 2010; 38(1): 96-109.   DOI
10 Bresciani G, Cruz IB, de Paz JA, Cuevas MJ, Gonzalez-Gallego J. The MnSOD Ala16Val SNP: relevance to human diseases and interaction with environmental factors. Free Radic Res 2013; 47(10): 781-792.   DOI
11 Duarte MM, Moresco RN, Duarte T, Santi A, Bagatini MD, Da Cruz IB, Schetinger MR, Loro VL. Oxidative stress in hypercholesterolemia and its association with Ala16Val superoxide dismutase gene polymorphism. Clin Biochem 2010; 43(13-14): 1118-1123.   DOI
12 Suvakov S, Damjanovic T, Stefanovic A, Pekmezovic T, Savic- Radojevic A, Pljesa-Ercegovac M, Matic M, Djukic T, Coric V, Jakovljevic J, Ivanisevic J, Pljesa S, Jelic-Ivanovic Z, Mimic-Oka J, Dimkovic N, Simic T. Glutathione S-transferase A1, M1, P1 and T1 null or low-activity genotypes are associated with enhanced oxidative damage among haemodialysis patients. Nephrol Dial Transplant 2013; 28(1): 202-212.   DOI
13 Knapen MF, Zusterzeel PL, Peters WH, Steegers EA. Glutathione and glutathione-related enzymes in reproduction. A review. Eur J Obstet Gynecol Reprod Biol 1999; 82(2): 171-184.   DOI
14 Celik SK, Aras N, Yildirim O, Turan F, Gorur A, Yildirim H, Tamer L. Glutathione S-transferase GSTM 1, null genotype may be associated with susceptibility to age-related cataract. Adv Clin Exp Med 2015; 24(1): 113-119.   DOI
15 Goodrich JM, Basu N. Variants of glutathione s-transferase pi 1 exhibit differential enzymatic activity and inhibition by heavy metals. Toxicol In Vitro 2012; 26(4): 630-635.   DOI
16 Brunst KJ, Baccarelli AA, Wright RJ. Integrating mitochondriomics in children's environmental health. J Appl Toxicol 2015; 35(9): 976-991.   DOI
17 Ahn K. The role of air pollutants in atopic dermatitis. J Allergy Clin Immunol 2014; 134(5): 993-999 .   DOI
18 McCrindle BW. Cardiovascular consequences of childhood obesity. Can J Cardiol 2015; 31(2): 124-130.   DOI
19 Akyol O, Canatan H, Yilmaz HR, Yuce H, Ozyurt H, Sogut S, Gulec M, Elyas H. PCR/RFLP-based cost-effective identification of SOD2 signal (leader) sequence polymorphism (Ala-9Val) using NgoM IV: a detailed methodological approach. Clin Chim Acta 2004; 345(1-2): 151-159.   DOI
20 Wilson MH, Grant PJ, Hardie LJ, Wild CP. Glutathione S-transferase M1 null genotype is associated with a decreased risk of myocardial infarction. FASEB J 2000; 14(5): 791-796.   DOI
21 Sutton A, Khoury H, Prip-Buus C, Cepanec C, Pessayre D, Degoul F. The Ala16Val genetic dimorphism modulates the import of human manganese superoxide dismutase into rat liver mitochondria. Pharmacogenetics 2003; 13(3): 145-157.   DOI
22 Becer E, Cirakoglu A. Association of the Ala16Val MnSOD gene polymorphism with plasma leptin levels and oxidative stress biomarkers in obese patients. Gene 2015; 568(1): 35-39.   DOI
23 Zimniak P, Nanduri B, Pikula S, Bandorowicz-Pikula J, Singhal SS, Srivastava SK, Awasthi S, Awasthi YC. Naturally occurring human glutathione S-transferase GSTP1-1 isoforms with isoleucine and valine in position 104 differ in enzymic properties. Eur J Biochem 1994; 224(3): 893-899.   DOI
24 Watson MA, Stewart RK, Smith GB, Massey TE, Bell DA. Human glutathione S-transferase P1 polymorphisms: relationship to lung tissue enzyme activity and population frequency distribution. Carcinogenesis 1998; 19(2): 275-280.   DOI
25 Aynacioglu AS, Nacak M, Filiz A, Ekinci E, Roots I. Protective role of glutathione S-transferase P1 (GSTP1) Val105Val genotype in patients with bronchial asthma. Br J Clin Pharmacol 2004; 57(2): 213-217.   DOI
26 Tamer L, Calikoglu M, Ates NA, Yildirim H, Ercan B, Saritas E, Unlu A, Atik U. Glutathione-S-transferase gene polymorphisms (GSTT1, GSTM1, GSTP1) as increased risk factors for asthma. Respirology 2004; 9(4): 493-498.   DOI
27 Jo HR, Lee HJ, Kang MH. Antioxidative status, DNA damage and lipid profiles in Korean young adults by glutathione S-transferase polymorphisms. Korean J Nutr 2011; 44(1): 16-28.   DOI
28 Lee E, Chang HY, Lee KS, Suh DI, Yu HS, Kang MJ, Choi IA, Park J, Kim KW, Shin YH, Ahn KM, Kwon JY, Choi SJ, Lee KJ, Won HS, Yang SI, Jung YH, Kim HY, Seo JH, Kwon JW, Kim BJ, Kim HB, Lee SY, Kim EJ, Lee JS, Keyes KM, Shin YJ, Hong SJ; COCOA study group. The effect of perinatal anxiety on bronchiolitis is influenced by polymorphisms in ROS-related genes. BMC Pulm Med 2014; 14: 154.   DOI
29 Reddy P, Naidoo RN, Robins TG, Mentz G, London SJ, Li H, Naidoo R. GSTM1, GSTP1, and NQO1 polymorphisms and susceptibility to atopy and airway hyperresponsiveness among South African schoolchildren. Lung 2010; 188(5): 409-414.   DOI
30 Karam RA, Pasha HF, El-Shal AS, Rahman HM, Gad DM. Impact of glutathione-S-transferase gene polymorphisms on enzyme activity, lung function and bronchial asthma susceptibility in Egyptian children. Gene 2012; 497(2): 314-319.   DOI
31 Gergen PJ, Fowler JA, Maurer KR, Davis WW, Overpeck MD. The burden of environmental tobacco smoke exposure on the respiratory health of children 2 months through 5 years of age in the United States: Third National Health and Nutrition Examination Survey, 1988 to 1994. Pediatrics 1998; 101(2): E8.
32 Yi O, Kwon HJ, Kim H, Ha M, Hong SJ, Hong YC, Leem JH, Sakong J, Lee CG, Kim SY, Kang D. Effect of environmental tobacco smoke on atopic dermatitis among children in Korea. Environ Res 2012; 113: 40-45.   DOI
33 Ministry of Health and Welfare, Korea Centers for Disease Control and Prevention. Korean adults male smoking rate [Internet]. Cheongju: Ministry of Health and Welfare; 2015 [cited 2015 Sep 8]. Available from : http://kosis.kr.
34 Forlenza MJ, Miller GE. Increased serum levels of 8-hydroxy-2'- deoxyguanosine in clinical depression. Psychosom Med 2006; 68(1): 1-7.
35 Holloway JW, Savarimuthu Francis S, Fong KM, Yang IA. Genomics and the respiratory effects of air pollution exposure. Respirology 2012; 17(4): 590-600.   DOI
36 Wu J, Hankinson J, Kopec-Harding K, Custovic A, Simpson A. Interaction between glutathione S-transferase variants, maternal smoking and childhood wheezing changes with age. Pediatr Allergy Immunol 2013; 24(5): 501-508.   DOI
37 Chiou CC, Chang PY, Chan EC, Wu TL, Tsao KC, Wu JT. Urinary 8-hydroxydeoxyguanosine and its analogs as DNA marker of oxidative stress: development of an ELISA and measurement in both bladder and prostate cancers. Clin Chim Acta 2003; 334(1-2): 87-94.   DOI
38 Carvalho AM, Miranda AM, Santos FA, Loureiro AP, Fisberg RM, Marchioni DM. High intake of heterocyclic amines from meat is associated with oxidative stress. Br J Nutr 2015; 113(8): 1301-1307.   DOI
39 Il'yasova D, Scarbrough P, Spasojevic I. Urinary biomarkers of oxidative status. Clin Chim Acta 2012; 413(19-20): 1446-1453.   DOI
40 Soto-Mendez MJ, Aguilera CM, Campana-Martin L, Martin- Laguna V, Schumann K, Solomons NW, Gil A. Variation in hydration status within the normative range is associated with urinary biomarkers of systemic oxidative stress in Guatemalan preschool children. Am J Clin Nutr 2015; 102(4): 865-872.   DOI
41 Oztop D, Altun H, Baskol G, Ozsoy S. Oxidative stress in children with attention deficit hyperactivity disorder. Clin Biochem 2012; 45(10-11): 745-748.   DOI
42 Omata N, Tsukahara H, Ito S, Ohshima Y, Yasutomi M, Yamada A, Jiang M, Hiraoka M, Nambu M, Deguchi Y, Mayumi M. Increased oxidative stress in childhood atopic dermatitis. Life Sci 2001; 69(2): 223-228.   DOI
43 Al-Alem U, Gann PH, Dahl J, van Breemen RB, Mistry V, Lam PM, Evans MD, Van Horn L, Wright ME. Associations between functional polymorphisms in antioxidant defense genes and urinary oxidative stress biomarkers in healthy, premenopausal women. Genes Nutr 2012; 7(2): 191-195.   DOI
44 Prasad SB, Vidyullatha P, Vani GT, Devi RP, Rani UP, Reddy PP, Prasad HM. Association of gene polymorphism in detoxification enzymes and urinary 8-OHdG levels in traffic policemen exposed to vehicular exhaust. Inhal Toxicol 2013; 25(1): 1-8.   DOI
45 He FF, Li HQ, Huang QX, Wang QY, Jiang HJ, Chen S, Su H, Zhang C, Wang YM. Tumor Necrosis Factor-Alpha and 8- Hydroxy-2'-Deoxyguanosine are Associated with Elevated Urinary Angiopoietin-2 Level in Type 2 Diabetic Patients with Albuminuria. Kidney Blood Press Res 2015; 40(4): 355-365.   DOI