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http://dx.doi.org/10.4163/jnh.2014.47.4.221

Fatty acid analysis and regulatory effects of citron (Citrus junos Sieb. ex TANAKA) seed oil on nitric oxide production, lipid accumulation, and leptin secretion  

Kim, Tae Woo (Well-being Bioproducts RIC, Kangwon National University)
Kim, Kyoung Kon (Department of Bio-Health Technology, Kangwon National University)
Kang, Yun Hwan (Well-being Bioproducts RIC, Kangwon National University)
Kim, Dae Jung (Well-being Bioproducts RIC, Kangwon National University)
Choe, Myeon (Well-being Bioproducts RIC, Kangwon National University)
Publication Information
Journal of Nutrition and Health / v.47, no.4, 2014 , pp. 221-228 More about this Journal
Abstract
Purpose: Citron seed oil (CSO) has been reported to have high antioxidant activity. However, the composition and other biologically activities of CSO have not been reported. In this study, we confirmed the fatty acid composition of CSO, which may be beneficial to vascular disease and obesity. Methods: We investigated the oil composition of CSO using gas chromatography coupled with mass spectrometry (GC-MS) analysis, and cytotoxicity was confirmed by Cell Counting Kit-8 (CCK-8) assay. Nitric oxide (NO) production in human umbilical vein endothelial cells (HUVECs) was measured using Griess reagent, and lipid accumulation and leptin secretion in 3T3-L1 cells were measured by Oil-Red O staining and commercial ELISA kit, respectively. Results: GC-MS analysis indicated that CSO contains several components, including linoleic acid, oleic acid, palmitic acid, stearic acid, linolenic acid, palmitoleic acid, and arachidic acid. In physiological activity analysis, CSO did not induce cytotoxic effects in HUVECs and 3T3-L1 cells. Further, CSO significantly induced nitric oxide and leptin secretion as well as inhibited lipid accumulation. Conclusion: CSO increased NO release, inhibited lipid accumulation, and induced leptin secretion, suggesting it may be useful for the management of vessels and weight gain. Although further studies are required to investigate the safety and mechanism of action of CSO, our results show that the composition and physiological activity of CSO are sufficient for its use as functional edible oil.
Keywords
citron seed oil; fatty acid composition; nitric oxide; leptin; lipid accumulation;
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1 Choi HS. Lipolytic effects of citrus peel oils and their components. J Agric Food Chem 2006; 54(9): 3254-3258.   DOI
2 Kim SH, Hur HJ, Yang HJ, Kim HJ, Kim MJ, Park JH, Sung MJ, Kim MS, Kwon DY, Hwang JT. Citrus junos Tanaka peel extract exerts antidiabetic effects via AMPK and PPAR-${\gamma}$ both in vitro and in vivo in mice fed a high-fat diet. Evid Based Complement Alternat Med 2013; 2013: 921012.
3 Hirota R, Roger NN, Nakamura H, Song HS, Sawamura M, Suganuma N. Anti-inflammatory effects of limonene from yuzu (Citrus junos Tanaka) essential oil on eosinophils. J Food Sci 2010; 75(3): H87-H92.   DOI
4 Kwon OC, Shin JH, Kang MJ, Lee SJ, Choi SY, Sung NJ. Antioxidant activity of ethanol extracts from citron (Citrus junos SIEB ex TANAKA) seed. J Korean Soc Food Sci Nutr 2006; 35(3): 294-300.   DOI
5 Lee SJ, Choi SY, Shin JH, Kim SH, Lim HC, Sung NJ. Fatty acid composition and oxidative stability of citron seed oils. J Life Sci 2006; 16(3): 427-432.   DOI   ScienceOn
6 Zhao G, Etherton TD, Martin KR, Vanden Heuvel JP, Gillies PJ, West SG, Kris-Etherton PM. Anti-inflammatory effects of polyunsaturated fatty acids in THP-1 cells. Biochem Biophys Res Commun 2005; 336(3): 909-917.   DOI   ScienceOn
7 Sales-Campos H, Souza PR, Peghini BC, da Silva JS, Cardoso CR. An overview of the modulatory effects of oleic acid in health and disease. Mini Rev Med Chem 2013; 13(2): 201-210.
8 Woo KS, Jeong JY, Hwang IG, Lee YJ, Lee YR, Park HJ, Park ES, Jeong HS. Antioxidant activity of ethanol extraction on citron seed by response surface methodology. J Korean Soc Food Sci Nutr 2009; 38(3): 384-390.   DOI
9 Kim SY, Shin KS. Evaluation of physiological activities of the citron (Citrus junos Sieb. ex TANAKA) seed extracts. Prev Nutr Food Sci 2013; 18(3): 196-202.   DOI
10 Lee YJ, Hwang IG, Joung EM, Kim HY, Park ES, Woo KS, Jeong HS. Physiological activity and antiproliferation effects of citron seed extracts on cancer cells. J Korean Soc Food Sci Nutr 2009; 38(12): 1672-1678.   DOI   ScienceOn
11 Kim DS, Kim DH, Oh MJ, Lee KG, Kook MC, Park CS. Antiaging and whitening activities of ethanol extract of Yuza (Citrus junos SIEB ex TANAKA) by-product. J Soc Cosmet Sci Korea 2010; 36(2): 137-143.
12 Minamisawa M, Yoshida S, Uzawa A. The functional evaluation of waste yuzu (Citrus junos) seeds. Food Funct 2014; 5(2): 330-336.   DOI
13 Choi WH, Um MY, Ahn JY, Jung CH, Seo JS, Ha TY. Effects of a rice-based diet on body weight and serum lipid levels in mice. J East Asian Soc Diet Life 2013; 23(1): 31-38.
14 Jang YS, Jeong JM. Effects of phyto-extract mixture on adiposity and serum lipid levels in obese mice induced by high fat diet. J Korean Soc Food Sci Nutr 2010; 39(10): 1439-1445.   DOI
15 Lim JK. Oil and fat industry. Food Ind 2004; 181: 10-37.
16 Zhang X, Scicli GA, Xu X, Nasjletti A, Hintze TH. Role of endothelial kinins in control of coronary nitric oxide production. Hypertension 1997; 30(5): 1105-1111.   DOI
17 Kang YH, Kang JS, Shin HM. Vasodilatory effects of cinnamic acid via the nitric oxide-cGMP-PKG pathway in rat thoracic aorta. Phytother Res 2013; 27(2): 205-211.   DOI   ScienceOn
18 Yu JH, Kim MS. Molecular mechanisms of appetite regulation. Diabetes Metab J 2012; 36(6): 391-398.   DOI
19 Hsu CL, Yen GC. Phenolic compounds: evidence for inhibitory effects against obesity and their underlying molecular signaling mechanisms. Mol Nutr Food Res 2008; 52(1): 53-61.   DOI   ScienceOn
20 Schwartz MW, Woods SC, Porte D Jr, Seeley RJ, Baskin DG. Central nervous system control of food intake. Nature 2000; 404 (6778): 661-671.   DOI
21 Lee SM, Kang YH, Kim DJ, Kim KK, Lim JG, Kim TW, Choe M. Comparison of antioxidant and ${\alpha}$-glucosidase inhibition activities among water extracts and sugar immersion extracts of green pepper, purslane and shiitake. J East Asian Soc Diet Life 2014; 24(1): 101-108.
22 Watkins BA, Shen CL, McMurtry JP, Xu H, Bain SD, Allen KG, Seifert MF. Dietary lipids modulate bone prostaglandin E2 production, insulin-like growth factor-I concentration and formation rate in chicks. J Nutr 1997; 127(6): 1084-1091.   DOI
23 Ministry of Food and Drug Safety. Korean food code [Internet]. Cheongju: Ministry of Food and Drug Safety; 2013 [cited 2014 Jul 21]. Available from: http://fse.foodnara.go.kr/residue/RS/ jsp/menu_02_01_01.jsp.
24 Oh SD, Kim M, Min BI, Choi GS, Kim SK, Bae H, Kang C, Kim DG, Park BJ, Kim CK. Effect of Achyranthes bidentata blume on 3T3-L1 adipogenesis and rats fed with a high-fat diet. Evid Based Complement Alternat Med 2014; 2014: 158018.
25 Kang ES, Ham SA, Hwang JS, Lee CK, Seo HG. Effects of Garcinia cambogia extract on the adipogenic differentiation and lipotoxicity. J Korean Soc Food Sci Anim Resour 2013; 33(3): 411-416.   DOI   ScienceOn
26 Maeda T, Horiuchi N. Simvastatin suppresses leptin expression in 3T3-L1 adipocytes via activation of the cyclic AMP-PKA pathway induced by inhibition of protein prenylation. J Biochem 2009; 145(6): 771-781.   DOI
27 Jeong JW, Kwon DJ, Hwang JB, Jo YJ. Influence of the extraction method on quality of citron juice. Korean J Food Sci Technol 1994; 26(6): 704-708.
28 Pasman WJ, Heimerikx J, Rubingh CM, van den Berg R, O'Shea M, Gambelli L, Hendriks HF, Einerhand AW, Scott C, Keizer HG, Mennen LI. The effect of Korean pine nut oil on in vitro CCK release, on appetite sensations and on gut hormones in post-menopausal overweight women. Lipids Health Dis 2008; 7: 10.   DOI   ScienceOn
29 Buys E, Sips P. New insights into the role of soluble guanylate cyclase in blood pressure regulation. Curr Opin Nephrol Hypertens 2014; 23(2): 135-142.   DOI
30 El-Mosallamy AE, Sleem AA, Abdel-Salam OM, Shaffie N, Kenawy SA. Antihypertensive and cardioprotective effects of pumpkin seed oil. J Med Food 2012; 15(2): 180-189.   DOI   ScienceOn
31 Hughes GM, Boyland EJ, Williams NJ, Mennen L, Scott C, Kirkham TC, Harrold JA, Keizer HG, Halford JC. The effect of Korean pine nut oil (PinnoThin(TM)) on food intake, feeding behaviour and appetite: a double-blind placebo-controlled trial. Lipids Health Dis 2008; 7: 6.   DOI   ScienceOn
32 Friedman JM, Halaas JL. Leptin and the regulation of body weight in mammals. Nature 1998; 395(6704): 763-770.   DOI   ScienceOn