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http://dx.doi.org/10.4163/jnh.2014.47.3.167

Study of the mechanisms underlying increased glucose absorption in Smilax china L. leaf extract-treated HepG2 cells  

Kang, Yun Hwan (Well-being Bioproducts RIC, Kangwon National University)
Kim, Dae Jung (Well-being Bioproducts RIC, Kangwon National University)
Kim, Kyoung Kon (Department of Bio-Health Technology, Kangwon National University)
Lee, Sung Mee (Department of Bio-Health Technology, Kangwon National University)
Choe, Myeon (Well-being Bioproducts RIC, Kangwon National University)
Publication Information
Journal of Nutrition and Health / v.47, no.3, 2014 , pp. 167-175 More about this Journal
Abstract
Purpose: Previous studies have shown that treatment with Smilax china L. leaf extract (SCLE) produces antidiabetic effects due to ${\alpha}$-glucosidase inhibition. In this study, we examined the mechanism underlying these antidiabetic effects by examining glucose uptake in HepG2 cells cultured with SCLE. Methods: Glucose uptake and glucokinase activity were examined using an assay kit. Expression of glucose transporter (GLUT)-2, GLUT-4, and HNF-$1{\alpha}$ was measured by RT-PCR or western blot. Results: Treatment with SCLE resulted in enhanced glucose uptake in HepG2 cells, and this effect was especially pronounced when cells were cultured in an insulin-free medium. SCLE induced an increase in expression of GLUT-2 but not GLUT-4. The increase in the levels of HNF-$1{\alpha}$, a GLUT-2 transcription factor, in total protein extract and nuclear fraction suggest that the effects of SCLE may occur at the level of GLUT-2 transcription. In addition, by measuring the change in glucokinase activity following SCLE treatment, we confirmed that SCLE stimulates glucose utilization by direct activation of this enzyme. Conclusion: These results demonstrate that the potential antidiabetic activity of SCLE is due at least in part to stimulation of glucose uptake and an increase in glucokinase activity, and that SCLE-stimulated glucose uptake is mediated through enhancement of GLUT-2 expression by inducing expression of its transcription factor, HNF-$1{\alpha}$.
Keywords
Smilax china. L; anti-diabetic activity; glucose uptake; glucose transporter; glucokinase;
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Times Cited By KSCI : 10  (Citation Analysis)
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