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http://dx.doi.org/10.4163/kjn.2011.44.4.284

Effects of Water Extracts of Red Pepper Seeds Powder on Antioxidative Enzyme Activities and Oxidative Damage in Rats Fed High-Fat and High-Cholesterol Diets  

Kim, Yu-Na (Department of Food Science, International University of Korea)
Ku, Kyung-Hyung (Korea Food Research Institute)
Kang, Sin-Kwon (Department of Food Science, International University of Korea)
Choi, Jeong-Hwa (Department of Food Science and Nutrition, International University of Korea)
Publication Information
Journal of Nutrition and Health / v.44, no.4, 2011 , pp. 284-291 More about this Journal
Abstract
The purpose of the present study was to examine the effects of water extracts from red pepper seeds powder on antioxidative enzyme activities and oxidative damage in groups of rrats fed high-fat and high-cholesterol diets group (HFC). The Rrats were divided into the following five experimental groups which are : composed of a normal diet group, a high fat high cholesterol diet group, and a high fat high cholesterol diet group supplemented with different amounts contents (1%, 2% and 4%) of red pepper seeds powder water extracts supplemented groups (HFCW1, HFCW2 and HFCW4, respectively). Body weight gains and food intake were lower ofin the red pepper seed water extracts groups were lower than those inof the HFC group. Hepartic xanthine oxidase (XOD) activity was decreased in the HFCW2 and HFCW4 groups compared to the HFC group. Hepartic glutathione peroxidase (GSH-px) activitiyactivity was increased in the HFCW4 group compared to the HFC group. Hepatic superoxide radicals within the mitochondria and microsomes of cells were significantly reduced in the HFCW2 and HFCW4 groups compared to the HFC group. Hepartic hydrogen peroxide in the cytosol was significantly reduced in the HFCW3 and HFCW4 groups compared to the HFC group. Hepatic carbonyl values in the microsomes and mitochondria were significantly reduced in the HFCW4 group compared to the HFC group. Hepartic thiobarbituric acid reaction substance (TBARS) activity was decreased in the HFCW2 group compared to the HFC group. These results suggest that water extracts of red pepper seeds powder may reduce oxidative damage by activation of antioxidative defense systems in rats fed high fat-high cholesterol diets.
Keywords
red pepper seeds powder; antioxidative system; high cholesterol; oxidative damage; free radical;
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Times Cited By KSCI : 16  (Citation Analysis)
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1 Rhee SJ, Ahn JM, Ku KH, Choi JH. Effects of radish leaves powder on hepatic antioxidative system in rats fed high-cholesterol diet. J Korean Soc Food Sci Nutr 2005; 34(8): 1157-1163   DOI
2 Lee YM, Bae JH, Jung HY, Kim JH, Park DS. Antioxidant activity in water and methanol extracts from korean edible wild plants. J Korean Soc Food Sci Nutr 2011; 40(1): 29-36   DOI
3 Liu RH. Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. Am J Clin Nutr 2003; 78(3 Suppl): 517S-520S   DOI
4 Yoon J, Jun JJ, Lim SC, Lee KH, Kim HT, Jeong HS, Lee J. Changes in selected components and antioxidant and antiproliferative activity of peppers depending on cultivation. J Korean Soc Food Sci Nutr 2010; 39(5): 731-736   DOI
5 Sim KH, Han YS. The Antimutagenic and Antioxidant effects of red pepper seed and red pepper pericarp (Capsicum annuum L). J Food Sci Nutr 2007; 12(4): 273-278   DOI
6 Choi SM, Jeon YS, Jung KO, Park KY. Antimutagenic effects of different kinds and parts of red pepper/powder on the Nmethyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced mutagenicities. J Korean Assoc Cancer Prev 2001; 6(2): 108-115
7 Ki HS, Han YS. Antioxidant activities of red pepper (Capsicum annuum) pericarp and seed extracts. Int J Food Sci Technol 2008; 43(10): 1813-1823   DOI   ScienceOn
8 Song WY, Yang JA, Ku KH, Choi JH. Effect of red pepper seeds powder on antioxidative system and oxidative damage in rats fed high-fat.high-cholesterol diet. J Korean Soc Food Sci Nutr 2009; 38(9): 1161-1166   DOI
9 Joo HY, Lim KT. Protective effect of glycoprotein isolated from Cudrania tricuspidata on liver in CCl4-treated A/J mice. Korean J Food Sci Technol 2009; 41(1): 93-99
10 Suh SH, Lee HR, Rhee SJ, Choi SW, Cho SH. Effects of peonia seed extracts and resveratrol on lipid metabolism in rats fed high cholesterol diets. J Korean Soc Food Sci Nutr 2003; 32(7): 1102-1107   DOI
11 Duke EJ, Joyce P, Ryan JP. Characterization of alternative molecular forms of xanthine oxidase in the mouse. Biochem J 1973; 131(2): 187-190   DOI
12 Ham YK, Kim SW. Protective effects of plant extract of plant extracts on the hepatocytes of rat treated with carbon tetrachloride. J Korean Soc Food Sci Nutr 2004; 33(8): 1246-1251   DOI
13 Urano S, Hoshi-Hashizume M, Tochigi N, Matsuo M, Shiraki M, Ito H. Vitamin E and the susceptibility of erythrocytes and reconstituted liposomes to oxidative stress in aged diabetics. Lipids 1991; 26(1): 58-61   DOI   ScienceOn
14 Kim HJ. Antioxidant and physialogical activities of capsicum annuum extract [dissertation]. Seoul: Korea University; 2010
15 Song WY, Ku KH, Choi JH. Effect of ethanol extracts from red pepper seeds on antioxidative defense system and oxidative stress in rats fed high-fat, high-cholesterol diet. Nutr Res Pract 2010; 4(1): 11-15   DOI
16 Park YS. Antioxidative activities and contents of polyphenolic compound of medicinal herb extracts. J East Asian Soc Diet Life 2002; 12(1): 23-31
17 Moon SH, Lee MK. Inhibitory effects of xanthine oxidase by boiled water extract and tannin from persimmon leaves. Korean J Food Nutr 2001; 11(3): 354-357
18 Kim SM, Jung YJ, Pan CH, Um BH. Antioxidant activity of methanol extracts from the genus lespedeza. J Korean Soc Food Sci Nutr 2010; 39(5): 769-775   DOI
19 Ku KH, Choi EJ, Park WS. Functional activity of water and ethanol extracts from red pepper (Capsicum annuum L.) seeds. J Korean Soc Food Sci Nutr 2008; 37(10): 1357-1362   DOI
20 Lee J, Lee SR. Analysis of phenolic substances content in Korean plant foods. Korean J Food Sci Technol 1994; 26(3): 310-316
21 Yeo SG, Park YB, Kim IS, Kim SB, Park YH. Inhibitory effects of xanthine oxidase by tea extract from green tee, oolong tea, and black tea. J Korean Soc Food Sci Nutr 1995; 24: 154-159
22 Sim KH, Kim SI, Cho YK, Cho YS, Han YS. The effects of red pepper seed on Kimchi quality during fermentation. Food Qual Cult 2007; 1(1): 27-33.
23 Ku KH, Choi EJ, Park JB. Chemical component analysis of red pepper (Capsicum annuum L.) seeds with various cultivars. J Korean Soc Food Sci Nutr 2008; 37(8): 1084-1089   DOI
24 Stirpe F, Corte ED. The regulation of rat liver xanthine oxidase. Conversion in vitro of the enzyme activity from dehydrogenase (type D) to oxidase (type O). J Biol Chem 1969; 244(14): 3855-3863
25 MarKlund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974; 47(3): 469-474   DOI   ScienceOn
26 Lawrence RA, Burk RF. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun 1976; 71(4): 952-958   DOI   ScienceOn
27 Aebi H, Wyss SR, Scherz B, Skvaril F. Heterogeneity of erythrocyte catalase II. Isolation and characterization of normal and variant erythrocyte catalase and their subunits. Eur J Biochem 1974; 48(1): 137-145   DOI   ScienceOn
28 Gay C, Gebicki JM. A critical evaluation of the effect of sorbitol on the ferric-xylenol orange hydroperoxide assay. Anal Biochem 2000; 284(2): 217-220   DOI   ScienceOn
29 Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, Ahn BW, Shaltiel S, Stadtman ER. Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol 1990; 186: 464-478
30 Azzi A, Montecucco C, Richter C. The use of acetylated ferricytochrome c for the detection of superoxide radicals produced in biological membranes. Biochem Biophys Res Commun 1975; 65(2): 597-603   DOI   ScienceOn
31 Satoh K. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clin Chim Acta 1978; 90(1): 37-43   DOI   ScienceOn
32 Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193(1): 265-275
33 Steel RGD, Torrie JH. Principles and procedures of statistics. New York, USA: McGraw-Hill; 1990
34 Zhang XH, Choi SK, Seo JS. Effect of dietary grape pomace on lipid oxidation and related enzyme activities in rats fed high fat diet. Korean J Nutr 2009; 42(5): 415-422   DOI
35 Ko SC, Kang SM, Ahn G, Yang HP, Kim KN, Jeon YJ. Antioxidant activity of enzymatic extracts from sargassum coreanum. J Korean Soc Food Sci Nutr 2010; 39(4): 494-499   DOI
36 Jang JR, Hwang SY, Lim SY. Effects of extracts from dried Yam on antioxidant and growth of human cancer cell lines. J Life Sci 2010; 20(9): 1365-1372   DOI
37 Yokozawa T, Nakagawa T, Kitani K. Antioxidative activity of green tea polyphenol in cholesterol-fed rats. J Agric Food Chem 2002; 50(12): 3549-3552   DOI   ScienceOn
38 Bok SH, Park SY, Park YB, Lee MK, Jeon SM, Jeong TS, Choi MS. Quercetin dihydrate and gallate supplements lower plasma and hepatic lipids and change activities of hepatic antioxidant enzymes in high cholesterol-fed rats. Int J Vitam Nutr Res 2002; 72(3): 161-169   DOI   ScienceOn
39 Balkan J, Kanbagli O, Hatipoglu A, Kucuk M, Cevikbaş U, Aykac-Toker G, Uysal M. Improving effect of dietary taurine supplementation on the oxidative stress and lipid levels in the plasma, liver and aorta of rabbits fed on a high-cholesterol diet. Biosci Biotechnol Biochem 2002; 66(8): 1755-1758   DOI   ScienceOn