Browse > Article
http://dx.doi.org/10.4163/kjn.2009.42.6.505

Effect of Vitamin C, Silicon and Iron on Collagen Synthesis and Break-Down Enzyme Expression in the Human Dermal Fibroblast Cell (HS27)  

Kim, Jeong-Eun (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Lee, Jin-Ah (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Kim, Hyun-Ae (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Kim, Jung-Min (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Cho, Yun-Hi (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
Publication Information
Journal of Nutrition and Health / v.42, no.6, 2009 , pp. 505-515 More about this Journal
Abstract
Collagen is the major matrix protein in dermis and consists of proline and lysine, which are hydroxylated by prolyl hydroxylase (PH) and lysyl hydroxylase (LH) with cofactors such as vitamin C, oxygen, iron (Fe$^{2+}$), ketoglutarate and silicon. The collagen degradation is regulated by matrix metalloproteinase-1 (MMP-1), of which is the major collagen-degrading proteinase whereas tissue inhibitors of metalloproteinase-1 (TIMP-1) bind to MMP-1 thereby inhibiting MMP-1 activity. In this study, we investigated the effects of vitamin C, silicon and iron on mRNA, protein expressions of PH, LH, MMP-1 and TIMP-1. The physiological concentrations of vitamin C (0-100 $\mu$M), silicon (0-50 $\mu$M) and iron (Fe$^{2+}$：0-50 $\mu$M) were treated to human dermal fibroblast cells (HS27 cells) for 3 or 5days. The expression level of mRNA and protein was increased in not only PH but also LH when cells were incubated with vitamin C. A similar increase in LH mRNA or protein expression occurred when cells were incubated with silicon. Our results suggest that treatment of vitamin C and silicon increased mRNA and protein expression of PH and LH in human dermal fibroblast.
Keywords
vitamin C; silicon; iron; prolyl hydroxylase; lysyl hydroxylase;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Elsner P, Wilhelm D, Maibach HI. Mechanical properties of human forearm and vulvar skin. Br J Dermatol 1990; 122: 607-614   DOI   ScienceOn
2 Prockop DJ, Berg RA, Kivirikko KI, Uitto J. Intracellular steps in the biosynthesis of collagen, Biochemistry of Collagen. In: Ramachandran GN, Reddi AH, editor. Plenum Press, New York; 1976. p.163-273
3 Jemec G, Jemec B, Jemec BIE. The effect of superficial hydration the mechanical properties of human skin in vivo. Plast Reconstr Surg 1990; 85: 100-103   DOI   ScienceOn
4 Passoja K, Rautavuoma K, Ala-Kokko L, Kosonen T, Kivirikko KI. Cloning and characterization of a third human lysyl hydroxylase isoform. Proc Natl Acad Sci USA 1998; 95(18): 10482-10486   DOI   ScienceOn
5 Che P, Xu J, Shi H, Ma Y. Quantitative determination of serum iron in human blood by high-performance capillary electrophoresis. J Chromatogr B Biomed Sci Appl 1995; 669: 45-51   DOI   ScienceOn
6 Fahling M, Perlewitz A, Doller A, Thiele BJ. Thiele. Regulation of collagen prolyl 4-hydroxylase and matrix metalloproteinases in fibrosarcoma cells by hypoxia. Comp Biochem Physiol Part C 2004; 139: 119-126   DOI   PUBMED   ScienceOn
7 Sim GS, Kim JH, Lee DH, Park SM. Pyo HB, Zhang YH, Lee BC. Effects of the Spatholobi Caulis extract on Antioxidation and inhibition of Matrix Metalloproteinase in Human Skin Fibroblasts. Korean J Biochnol Bioeng 2005; 20(1): 40-45   과학기술학회마을
8 Murad S, Sivarajah A, Pinnell SR. Prolyl and lysyl hydroxylase activities of human skin fibroblast: effect of donor age and ascorbate. J Invest Dermatol 1980; 75: 404-407   DOI   PUBMED   ScienceOn
9 Duarte TL, Almeida GM, Jones GD. Investigation of the role of extracellular H2O2 and transition metal ions in the genotoxic action of ascorbic acid in cell culture models. Toxicol Lett 2007; 170(1): 57-65   DOI   ScienceOn
10 Kim NM, Koo BS, Lee SK, Hwang EI, So SH, Do JH. Effect of Korean red ginseng on collagen biosynthesis and MMP-Ⅰactivity in human dermal fibroablast. J Ginseng Res 2007; 31(2): 86-92   DOI   ScienceOn
11 Tajima S, Pinnel SR. Ascorbic acid preferentially enhances type I and III collagen gene transcription in human skin fibroblasts. J Dermatol Sci 1996; 11: 250-253   DOI   ScienceOn
12 Seaborn CD, Nielsen FH. Silicon deprivation decreases collagen formation in wounds and bone, and ornithine transaminase enzyme activity in liver. Biol Trace Elem 2002; 89(3): 251-261   DOI   ScienceOn
13 Ma XB, Sun SX, Yang F. Effect of human silicotic alveolar macrophages on the expression of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinases-1 in human lung fibroblasts. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2004; 22 (5): 358-360   PUBMED
14 Tuderman L, Myllo R, Kivirikko KI. Mechanism of the prolyl hydroxylase reaction. I. Role of co-substrates. Eur J Biochem 1977; 80: 341-348   DOI   ScienceOn
15 Pinnell SR, Murad S, Darr D. Induction of collagen synthesis by ascorbic acid. A possible Mechanism. Arch Dermatol 1987; 123: 1684-1686   DOI   ScienceOn
16 Uzawa K, Grzesik WJ, Nishiura T, Kuznetsov SA, Robey PG, Brenner DA, Yamauchi M. Differential expression of human lysyl hydroxylase genes, lysine hydroxylation, and cross-linking of type I collagen during osteoblastic differentiation in vitro. J Bone Miner Res 1999; 14(8): 1272-1280   DOI   ScienceOn
17 Kao WW, Flaks JG, Prockop DJ. Primary and secondary effects of ascorbate on procollagen synthesis and protein synthesis by primary cultures of tendon fibroblasts. Arch Biochem Biophys 1976; 173: 638-648   DOI   ScienceOn
18 Fisher GJ, Voorhees JJ. Molecular mechanism of photoaging in human skin in vivo and their prevention by all-trans retinoic acid. Photochem Photobiol 1999; 69(2): 154-157   DOI   ScienceOn
19 Carlisle EM. Silicon: a requirement in bone formation independent of vitamin D. Calcif Tissue Int 1981; 33: 27-34   DOI   PUBMED   ScienceOn
20 Enjoji M, Kotoh K, Iwamoto H, Nakamuta M, Nawata H. Selfregulation of TypeⅠcollagen degradation by collagen-induced production of matrix metalloproteinase-1 on cholangiocarcinoma and hepatocellular carcinoma cells. In vitro Cell Dev Biol Anim 2000; 36: 71-73   DOI   ScienceOn
21 Ronni C. Micronutrient requirements in older women. Am J Clin Nutr 2005; 81: 1240-1245   DOI
22 Campo GM, Avenoso A, Campo S, D'Ascola A, Ferlazzo AM, Sama D, Calatroni A. Purified human chondroitin-4-sulfate reduced MMP/TIMP imbalance induced by iron plus ascorbate in human fibroblast cultures. Cell Biol Int 2006; 30(1): 21-30   DOI   PUBMED   ScienceOn
23 Barel A, Calomme M, Timchenko A, De Paepe K, Demeester N, Rogiers V, Clarys P, Vanden Berghe D. Effect of oral intake of choline-stabilized orthosilicic acid on skin, nails and hair in women with photodamaged skin. Arch Dermatol Res 2005; 297 (4): 147-53   DOI   ScienceOn
24 Uzawa K, Yeowell HN, Yamamoto K, Mochida Y, Tanzawa H, Yamauchi M. Lysine hydroxylation of collagen in a fibroblast cell culture system. Biochem Biophys Res Commun 2003; 305: 484-487   DOI   ScienceOn
25 Boyera N, Galey I, Bernard BA. Effect of vitamin C and its derivatives on collagen synthesis and cross-linking by normal human fibroblasts. Int J Cosmet Sci 1998; 20: 151-158   DOI   ScienceOn
26 Matsumura Y, Ananthaswamy HN. Toxic effects of ultraviolet radiation on the skin. Toxicol Appl Pharmacol 2004; 195: 298-308   DOI   ScienceOn
27 Fisher GJ, Kang S, Varani J, Bata-Csorgo Z, Wan Y, Datta S, Bpprjees JJ. Mechanisms of photoaging and chronological skin againg. Arch Dermatol 2002; 138: 1462-1470   DOI   ScienceOn
28 Murad S, Tajima S, Johnson GR, Sivarajah S, Pinnell SR. Collagen synthesis in cultured human skin fibroblasts: Effect of ascorbic acid and its analogs. J Invest Dermatol 1983; 81: 158-162   DOI   ScienceOn
29 Wlaschek M, Tantcheva-Poor I, Naderi L, Ma W, Schneider LA, Razi-Wolf Z, Schuller J, Scharffetter-Kochanek K. Solar UV irradiation and dermal photoaging. J Photochem Photobio 2001;63(1-3): 41-51   DOI   ScienceOn
30 Fisher GJ, Kang SW, Voorhees JJ. Retinoic acid inhibits induction of c-Jun protein by ultraviolet radiation that occur subsequent to activation of mitogen-activated protein kinase pathways in human skin in vivo. J Clin Invest 1998; 101 (6): 1432-1440   DOI   ScienceOn
31 Switzer BR, Summer GK. Collagen synthesis in human skin fibroblasts: effects of ascorbate, -ketoglutarate and ferrous ion on proline hydroxylation. J Nutr 1972; 102(6): 721-728   DOI   PUBMED
32 Oono T, Shirafuji Y, H WK, Akiyama H, Iwatsuki K. Effects of human neutrophil peptide-1 on the expression of interstitial collagenase and type I collagen in human dermal fibroblasts. Arch Dermatol Res 2002; 294: 185-189   DOI   ScienceOn