Browse > Article

Effect of Cyanidin on Cell Motility and Invasion in MDA-MB-231 Human Breast Cancer Cells  

Chu, Su-Kyoung (Department of Nursing, Eulji University)
Seo, Eun -Young (Department of Food Science & Nutrition, Dankook University)
Kim, Woo-Kyoung (Department of Food Science & Nutrition, Dankook University)
Kang, Nam-E (Department of Food & Nutrition, Eulji University)
Publication Information
Journal of Nutrition and Health / v.41, no.8, 2008 , pp. 711-717 More about this Journal
Abstract
Anthocyanidins, the aglycones of anthocyanins, are natural colorants belonging to the flavonoid family. Cyanidin is one of the anthocyanidins, used for their antioxidant properties. Furthermore, previous studies have shown anthocyanidin-rich material extracts or aglycone form inhibit growth and induce apoptosis of cancer cells. But, Tumor metastasis is the most important cause of cancer death, and various treatment strategies have targeted on preventing the occurrence of metastasis. This study investigated the effects of cyanidin on metastasis processes, including motility, invasion and activity of MMP-2 and MMP-9 in MDA-MB-231 human breast cancer cell lines. We cultured MDA-MB-231 cells in presence of various concentrations 0, 5, 10 and 20 ${\mu}M$ of cyanidin. The cell motility was significantly decreased dosedependently in cells treated with cyanidin (p < 0.05) and cyanidin treatment caused the significant suppression of the invasion (p < 0.05). MMP-2 and MMP-9 activities, and MMP-9 mRNA express were not affected by anthocyanin treatment. In conclusion, cyanidin inhibits cell motility, invasion in MDA-MB-231 human breast cancer cell lines.
Keywords
cyanidin; metastasis; motility; invasion; breast cancer;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Korea National Statistical Office. Deaths by cause; 2005
2 Bae , Moon . A study of the antioxidatives of Korean soybeans. J Korean Soc Food Sci Nutr 1997; 26: 203-208
3 Wang H, Cao G, Prioi RL. Oxygen radical absorbing capacity anthocyanins. J Agric Food Chem 1999; 45: 304-309   DOI   ScienceOn
4 Ichiyanagi T, Hatano Y, Matsugo S, Konishi T. Stimultaneous comparison of reactive nitrogen species. Chem Pham Bull(Tokyo) 2004;52: 1312-1325   DOI   ScienceOn
5 Kahkonen MP, Heinonen M. Antioxidant activity of acthocyanins and their aglycones. J Agric Food Chem 2003; 51: 628-633   DOI   ScienceOn
6 Yoon TH, Lee SW. Stability of anthocyanins in foods. J Korean Food Sci Technol 1979; 11: 63-73
7 Seo EY, Lee HS, Kim WK. Effect of [6]-gingerol on onhibition of cell proliferation in MDA-MB-231 human breast cancer cells. Korean J Nutr 2005; 38(8): 656-662   과학기술학회마을
8 Landesberg R, Cozin M, Cremers S, Woo V, Kousteni S, Sinha S, Garrett-Sinha L, Raghavan S. Inhibition of oral mucosal cell wound healing by bisphosphonates. J Oral Maxillofac Surg 2008; 66 (5): 839-847   DOI   ScienceOn
9 Bartsch JE, Staren ED, Appert HE. Matrix metalloproteinase expression in breast cancer. J Surg Res 2003; 110: 383-392   DOI   ScienceOn
10 Yanjun Z, Vareed Shaiju KV, Muraleedharan GN. Human tumor cell growth inhibition by nontoxic anthocyanidins, the pigments in fruits and vegetables. Life Sci 2005; 76: 1465-1472   DOI   ScienceOn
11 Ye X, Krohn RL, Liu W, Joshi SS, Kuszynski CA, McGinn TR, Bagchi M, Preuss HG, Stohs SJ, Bagchi D. The cytotoxic effects of a novel IH 636 grape seed proanthocyanidin extract on cultured human cancer cells. Mol Cell Biochem 1999; 196(1-2): 99-108   DOI
12 Chen PN, Kuo WH, Chiang CL, Chiou HL, Hsieh YS, Chu SC. Black rice anthocyanins inhibit cancer cells invasion via repressions of MMPs and u-PA expression. Chem Biol Interact 2006; 163: 218-229   DOI   ScienceOn
13 Morini M, Mottolese M, Ferarri N, Ghiorzo F, Buglioni S, Mortarini R. The $\alpha3 $ $\beta1$ integrin is associated with mammary carcinoma cell metastasis, invasion and gelatinase B (MMP-9) activity. Int J Cancer 2000; 87: 336-342   DOI   ScienceOn
14 Nagase H, Sasaki K, Kito H, Sato T. Inhibitory effect of delphinidin from solanum melongena on human fibrosarcoma HT-1080 invasiveness in vitro. Planta Med 1998; 64(3): 216-219   DOI   ScienceOn
15 Nagase H. Activation mechanism of matrix metalloproteinase. Biol Chem 1997; 378: 151-160
16 Weng MS, Ho CT, Ho YS, Lin JK. Theanaphthoquinone inhibits fatty acid synthase expression in EGF-stimulated human breast cancer cells via regulation of EGFR/EerB-2 signaling. Toxicol appl pharmacol 2007; 218: 107-118   DOI   ScienceOn
17 Kim WK, Park EH, Han WK. A follow up study on colorectal cancer. Korean J Surg Soc 1986; 31(2): 191-199
18 Suh CS, Moon SY. Apoptosis and expression of bcl-2, bax in the cyclic human endometrium. Korean J Obstet Gynecol 1999; 45 (11): 2446-2457
19 Sylvie L, Rene L, Valerie B, Albert M, Stephane B, Denis G, Richard B. Anthocyanidins inhibit migration of glioblastoma cells: structure-activity relationship and involvement of the plasminolytic system. J Cell Biochem 2007; 100: 100-111   DOI   ScienceOn
20 Polette M, Nawrocki-Raby B, Gilles C, Clavel C, Birembaut P. Tumor invasion and matrix metalloproteinase. Crit Rev Oncol Hematol 2004; 49(3): 179-186   DOI   ScienceOn
21 Woodhouse EC, Chuaqui RF, Liotta LA. General mechanism of metastasis. Cancer 1997; 80: 1529-1537   DOI   ScienceOn
22 Ichiyanagi T, Hatano Y, Matsugo S, Konishi T. Comparison of anthocyanin reactivity towards hydroxyl radical, superoxide anion and singlet oxygen. ITE Lett 2003; 4: 788-793
23 Yoshimoto M, Okuno S, Yamaguchi M, Yamakawa O. Antimutagenicity of deacylated anthocyanins in purple-fleshed sweetpotato. Biosci Biotechnol Biochem 2001; 65: 1652-1655   DOI   ScienceOn
24 Huh KB. The present status of nutrition related diseases and its countermeasures. Korean J Nutr 1990; 23(3): 197-207
25 Aye MM, Ma C, Lin H, Bower KA, Wiggins RC, Luo J. Ethanolinduced in vitro invasion of breast cancer cells: the contribution of MMP-2 by fibroblasts. Int J Cancer 2004; 112: 738-746   DOI   ScienceOn
26 Bang MH. Effects of some phytochemicals on cell invasion and matrix metalloproteinase (MMP) activity in MDA-MB-231 human breast cancer cells [MS thesis]. Seoul: Dankook university; 2004
27 Harbone JB. Comparativebiochemistry of the flavonoids. Academic Press; 1967
28 Unemori EN, Werb Z. Reorganization of polymerized action; a possible trigger for induction of procollagenase in fibroblasts cultured in and on collagen gels. J Cell Biol 1986; 103(3): 1021-1031   DOI   ScienceOn
29 Matchett MD, Mackinnon SL, Sweeney MI, Gottschall-Pass KT, Hurta RA. Blueberry flavonoids inhibit matrix metalloproteinase activity in DU145 human prostate cancer cells. Biochem Cell Biol 2005; 83(5): 637-643   DOI   ScienceOn
30 Min D, Rentian F, Shiow YW, Linda B, Yongju L, Yong Q, Vincent C, Bing HJ, Xianglin S. Cyanidin-3-glucoside, a natural product derived from blackberry, exhibits chemopreventive and chemotherapeutic activity. J Biol Chem 2006; 25: 17359-17368
31 Emma MC, Gina P, Chris IG, Mark M, Gordon JM, Derek S, Ian R. Colon-available raspberry polyphenols exhibit anti-cancer effects on in vitro models of colon cancer. Carcinogenesis 2007; 6 (4): 1-13   DOI   ScienceOn
32 Chen PN, Chu SC, Chiou HL, Kuo WH, Chiang CL, Hsieh YS. Mulberry anthocyanidin 3-rutinoside and cyanidin 3-glucoside, exhibited an inhibitory effect on the migration and invasion of a human lung cancer cell line. Cancer Let 2006; 235(2): 248-259   DOI   ScienceOn
33 Ryu YS. A study on dietary factor related to the incidence of breast cancer and of cervix cancer in Korea women [MS thesis]. Seoul: Ewha Womans University; 1992
34 Stetler-Stervenson W, Aznavoorian S, Liotta L. Tumor cell interactions with the extracellular matrix during invasion and metastasis. Annu Rev Cell Dev Biol 1993; 9: 541-573   DOI
35 Taraboletti G, Roberts D, Liotta L. Thrombospondin-induced tumor cell migration: haptotaxis and chemotaxis are mediated by different molecular domains. J Cell Biology 1987; 105: 2409-2415   DOI   ScienceOn
36 Kong JM, Chia LS, Goh N, Chia T, Brouillard R. Analysis and biological activities of anthocyanins. Phytochemistry 1993; 64: 923-933   DOI   ScienceOn
37 Glasspool RM, Twelves CJ. Matrix metalloproteinase inhibitors: past lessons and future prospects in breast cancer. Breast 2001; 10 (5): 368-378   DOI   ScienceOn
38 Francesco C, Roberto A, Raffaella C, Antonia S, Fabio V. Effect of cyanidin-3-O-glucoside on UVB-induced response in human keratinocytes. J Agric Food Chem 2006; 54: 4041-4047   DOI   ScienceOn
39 Hou DX. Potential mechansm of cancer chemoprevention by anthocyanins. Curr Mol Med 2003; 3: 149-159   DOI   ScienceOn
40 Katsube N, Iwashita K, Tsushida T, Yamaki K, Kobori M. Induction of apoptosis in cancer cells by billberry or blueberry (Vaccinium myrtillus) and the anthocyanins. J Agric Food Chem 2003; 51: 68-75   DOI   ScienceOn
41 Bang MH, Kim JH, Kim WK. Effects of epigallocatechin gallate on adhesion, invasion and matrix matalloproteinase activity in MDA-MB-231 human breast cancer cells. Korean J Nutr 2005; 38(2): 104-111   과학기술학회마을
42 Wagner H. Annual proceedings of phytochemical society of Europe 1985; 25: 409