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Effects of Salicornia Herbacea L. Supplementation on Antioxidative Enzyme Activities in Streptozotocin-Induced Diabetic Rats  

Kim, Myung-Wha (Department of Food and Nutrition, Duksung Women's University)
Publication Information
Journal of Nutrition and Health / v.41, no.7, 2008 , pp. 583-593 More about this Journal
Abstract
This study was designed to examine the effects of Salicornia herbacea L. (glasswort: GW) on hepatic antioxidative enzyme activities in diabetic rats. Diabetes mellitus was induced in male Sprague-Dawley rats weighing 200-220g by an injection of streptozotocin (STZ) dissolved in a citrate buffer into the tail vein at a dose of 45 mg/kg of body weight. Sprague-Dawley rats were fed an AIN-93 recommended diet and the experimental groups were fed a modified diet containing 10% and 20% of glasswort powder for 4 weeks. The experimental groups were divided into 6 groups which consisted of normal (N)-control group, N-GW 10% and N-GW 20% treated groups, STZ-control, STZ-GW 10% and STZ-GW 20% treated groups. The activities of Xanthine oxidase (XOD), glutathione- S-transferase (GST), glutathione peroxidase (GPX), glutathione reductase (GR), superoxide dismutase (SOD) and CAT (CAT) were measured in the homogenates of liver. The activity of CAT was lower in the supplementary group with glasswort compare to the STZcontrol group but it was not significantly different. The activity of SOD was not significant in all of experimental groups. The activity of GR was significantly increased in the normal supplementary group with glasswort, and GPX activity was significantly increased in STZ-GW 10% group compare to the STZ-control group. The activity of XOD was significantly decreased in the all of supplementary groups with glasswort. The activity of GST was significantly increased in the N-GW 20% group and it was significantly decreased in the STZ-GW 20% group. These results show that the supplementation of glasswort may have favorable influence on antioxidative status in diabetic rats and it may be useful for the diabetic complications as functional food.
Keywords
salicornia herbacea L. (glasswort); diabetes mellitus; streptozotocin; antioxidative enzyme activities;
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1 Korea statistical information system, Korea statistical year book, Seoul; 2005
2 Maklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974; 47(3): 467-474
3 Videla LA, Fernandez V. Biochemical aspects of cellular oxidative stress. Arch Biol Med Exp 1988; 21(1): 85-92
4 Jo YC, Ahn JH, Chon SM, Lee KS, Bae TJ, Kang SK. Studies of pharmacological effects of glasswort (Salicornia herbacea L.). Korean J Medicinal Crop Sci 2002; 10(2): 93-99
5 Lee JT, An BJ. Detection of physical activity of salicornia herbacea L. Kor J Herbology 2002; 17(2): 61-69
6 Jang MS, Park JE. Optimization of ingredient mixing ratio for preparation of sulgidduk with saltwort (Salicornia herbacea L.). J Korean Soc Food Sci Nutr 2006; 35(5): 641-648   과학기술학회마을   DOI
7 Song TC, Lee CH, Kim YE, Kim IH, Han DS, Yang DH. The functionality of the saltwort (Salicornia herbacea L.) extract fermented juice. J Korean Soc Food Sci Nutr 2007; 36(4): 395-399   과학기술학회마을   DOI
8 Kwon YJ, Rhee SJ, Chu JW, Choi SW. Comparison of radical scavenging activity of extracts of mellberry juice and cake prepared from mulberry (morus spp.) fruit. Korean J Nutr 2005; 10(2): 111-117
9 Kim MW. Effects of salicornia herbacea L. supplementation on lipid peroxidation and mineral levels in streptosotocin-induced diabetic rats. Korean J Nutr 2007; 40(5): 403-412   과학기술학회마을
10 Junod A, Lambert AE, Stauffacher W, Renold AE. Diabetogenic action of streptozotocin: relationship of dose to metabolic response. J Clin Invest 1969; 48(11): 2129-2139   DOI
11 Samson M, Fehlmann M, Dolais-Kitabgi J, Freychet P. Amino acid transport in isolated hepatocytes from streptozotocin diabetic rats. Diabetes 1980; 29(2): 996-1000   DOI   ScienceOn
12 Bergmeyer HU, Gawehn K, Grassl M. In methods of enzymatic analysis (Bergmeter. HU Ed.). 2nd ed. Vol 1. Academic Press Inc. New York; 1974
13 Park GY, Lee SJ, Kim JG. Effects of green tea catechin on cytochrome p450, xanthine oxidase activities in liver and liver damage in streptozotocin induced diabetic rats. J Korean Soc Food Sci Nutr 1997; 26(5): 901-907
14 Lee YR, Kang MY, Nam SH. Effect of giant embryonic rice supplementation on the lipid peroxide levels and antioxidative enzyme activities in the plasma and liver of streptozotocin-induced diabetic rats. J Korean Soc Appl Biol Chem 2005; 48(4): 358-363
15 Kang NE, Kim WK. Effects of antioxidant vitamins supplementation on antioxidative status and plasma lipid profiles in Korean NIDDM patients. Korean J Nutr 1999; 32(7): 775-780
16 Lee YS, Lee HS, Shin KH, Kim BK, Lee SH. Constituents of the halophyte salicornia herbacea L.. Arch Pham Res 2004; 27 (10): 1034-1036   DOI
17 Fridovich I. The biology of oxygen radicals, the superoxide radical is an agent of oxygen toxicity: superoxide dismutase provide an important defense. Science 1978; 201(4359): 875-882   DOI
18 Tapan KB, Norman JT, Manohar LG. Antioxidants in human health and disease. CAB International, London, UK; 1999
19 Atalay M, Laaksonen DE, Niskanen L, Uusiyupa M, Hanninen O, Sen CK. Altered antioxidant enzyme defence in insulin-dependent diabetic men with increased resting and exercise induced oxidative stress. Acta Phsiologica Scandinavica 1997; 161(2): 195-201   DOI   ScienceOn
20 Cho YJ, Kim HA, Bang MA, Kim EH. Effects of dietary mushroom polysaccharides on blood glucose levels and energy metabolism in streptozotocin-induced diabetic rats. Korean J Nutr 2002; 35(2): 183-191
21 Bang MA, Kim HA, Cho YJ. Hypoglycemic and antioxidant effect of dietary hamcho powder in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 2002; 31(5): 840-846   DOI
22 Park SW, Kim DJ, Min KW, Baik SH, Choi KM, Park JB, etc. Current status of diabetes management in Korea using national health insurance database. J Korean Diabetes Assoc 2007; 31(4): 362-367   DOI
23 Borrpart GJ, Prevot DS, Bascands JL. Rapid automated analysis of glutathione reductase, peroxidase and S-transferase activity: application to cisplatin-induced toxicity. Clin Biochem 1990; 23 (6): 501-504   DOI   ScienceOn
24 Lawrence RA, Burk RF. Glutathione peroxidase activity in selenium deficient rat liver. Biochem Biophys Res Comm 1976; 71(4): 952-958   DOI   ScienceOn
25 Min JG, Lee DS, Kim TJ, Park JH. Chemical composition of Salicornia herbacea L. J Food Sci Nutr 2002; 7(1): 105-107   DOI
26 Han SK, Kim SM. Antioxidative effect of salicornia herbacea L. grown in closed sea beach. J Korean Soc Food Sci Nutr 2003; 32(2): 207-210   DOI
27 Wilson GL. Mechanism of streptozotocin-induced and alloxaninduced damage in rat $\beta$-cells. Diabetologia 1984; 27(6): 587-591   DOI   ScienceOn
28 Kinalski M, Seledziewski A, Telejko B, Zarzycki W, Kinalska I. Lipid peroxidation and scavenging enzyme activity in streptozotocin-induced diabetes. Acta Diabetol 2000; 37(4): 179-183   DOI   ScienceOn
29 Hammers HD. Aminoguanidine treatment inhibits the development of edible and medicinal diabetic retinopathy. Proc Natl Acad Sci USA 1991; 88(63): 11555-11558
30 Shin KS, Boo HO, Jean MW. Chemical components of native plant, Salicorinia herbacea L.. Korean J Plant Res 2002; 15(3): 216-220
31 Ramanathan M, Jaiswal AK, Bhattacharya SK. Superoxide dismutase, catalase and glutathione peroxidase activities in the brain of streptozotocin induced diabetic rat. Indian J Exp Biol 1999; 37(2): 182-183
32 Aebi H. Catalase in vitro. Meth Enzymol 1984; 105: 121-126   DOI
33 Reeves PG. Component of the AIN-93 diets as improvements in the AIN-76A diet. J Nutr 1997 ; 127(5 Suppl): 838S-841S   DOI
34 Kim MJ, Cho SY, Lee MK, Shin KH. Effects of Aralia elata water extracts on activities of hepatic oxygen free radical generating and scavenging enzymes in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 2004; 33(4): 653-658   DOI
35 Hermenegildo C, Raya A, Roma J, Romero FJ. Decrease glutathione peroxidase activity in sciatic nerve of alloxan-induced diabetic mice and its correlation with blood glucose levels. Neurochem Res 1993; 18(8): 893-896   DOI   ScienceOn
36 Habig WH, Pabst MJ, Jakoby WB. Glutathione-S-transferases: The first enzymatic step in mercapturic acid formation. J Biol Chem 1974; 249(22): 7130-7139
37 Sungpil Ryu, Yoon JT. Effects of green-tea polyphenol ingestion before exercise on antioxidative defense system in streptozotocininduced diabetic rats. Korean J Exercise Nutr 2002; 6(3): 271-276
38 Kim SK, Kim YC. The effect of repeated betaine treatment on hepatotoxicity and cytochrome P-450 dependent drug metabolism enzyme system. Yakhak Hoeji 1996; 40(4): 449-455
39 Choi JK. A edible Korean mdicinal herbs. J Korean Soc Plants People & Environ 2002; 5(1): 7-19
40 Fujita H, Haseyama T, Kayo T, Nosaki J, Wada Y, Ito S, Koizumi A. Increased expression of glutathione s-transferase in renal proximal tubules in the early stage of diabetes: a study of type 2 diabetes in the akita mouse model. Exp Nephrol 2001; 9(6): 380-386   DOI   ScienceOn
41 Chow CK. Nutritional influence on cellular antioxidant defense system. J Biol Chem 1969; 244(15): 6049-6055
42 Lee HS, Kim MH, Lee DH, Sung CJ. Relationship between some blood parameters and antioxidant enzyme activity in Korean postmenopausal women. J Korean Nutr 2006; 39(5): 476-484   과학기술학회마을
43 Shay G. Saline agriculture: salt-tolerant plants for developing countries. Washington: National academy press; 1990. p.143
44 Lee CH, Kim IH, Kim YE, Oh SW, Lee HJ. Determination of betaine from Salicornia herbacea L.. J Korean Soc Food Sci Nutr 2004; 33(9): 1584-1587   DOI
45 Kakkar R, Kalra J, Mantha SV, Prasod K. Lipid peroxidation and antioxidant enzymes in diabetic rats. Mol Cell Biochem 1995; 151(2): 113-119   DOI   ScienceOn
46 Park YR, Rhee SJ, Lin S, Joo GJ. Effects of dietary vitamin E on the microsomal mixed function oxidase system of liver and lung in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 1996; 25(6): 969-975
47 Jang YS, Ahn HS, Kim HR. Effects of vitamin E supplementation on the lipid peroxides and activities of antioxidative enzymes in the pancreas of diabetic KK mice. Korean J Nutr 1998; 31 (2): 153-158
48 Kim MW. Effects of salicornia herbacea L. supplementation on blood glucose and lipid metabolites in streptosotocin-induced diabetic rats. Korean J Nutr 2007; 40(1): 5-13   과학기술학회마을
49 Kwon EH, Jang HS, Kim SW, Choi SW, Rhee SJ, Cho SH. Effects of mulberry juice and cake powders on blood glucose and lipid lowering and erythrocytic antioxidative enzyme activities in streptozotocin-induced diabetic rats. J Korean Diabetes Assoc 2007; 40(3): 199-210   과학기술학회마을
50 Kim KR, Choi JH, Lee SK, Woo MH, Choi SW. Effect of enzymatic hydrolysate of Hamcho (Salosornia herbacea L.) on antioxidative defense system in rats fed high cholesterol diet. J Korean Soc Food Sci Nutr 2006; 35(10): 1356-1362   과학기술학회마을   DOI
51 Lowry OH, Rosebrough NJ, Farr AJ, Randall RR. Protein measurement with the foline phenol reagent. J Biol Chem 1951; 193 (1): 265-273
52 Halliwell B, Gutteridge MC. Free radicals in biology and medicine. Oxford University Press. Oxford; 1985. p.166-170
53 Wolff SP, Jiang ZY, Hung JV. Protein glycation and oxidative stress in diabetes mellitus and aging. Free Radical Biol Med 1991; 10(5): 339-352   DOI   ScienceOn
54 Zimmet P, Alberti KG, Shaw J. Global and societal implications of the diabetes epidemic. Nature 2001; 414(6865): 782-787   DOI   ScienceOn
55 Baynes JW. Role of oxidative stress in the development of complication in diabetes. Diabetes 1991; 40(4): 405-421   DOI   ScienceOn
56 Wobaieb SA, Godin DV. Alterations in tissue antioxidant systems in the spontaneously diabetic (BB Wistar) rat. Can J Physiol Pharmacol 1987; 65(11): 2191-2195   DOI
57 Im JS, Lee SK, Chang IY, Ha HC, Lim Y, Kim HY, Park KH, Yoon SP. Activation of macrophage by polysaccharide isolated from salicornia herbacea L.. Korean J Phys Anthropol 2006; 19 (2): 117-124   DOI
58 Yoo SK, Rhee SJ. Effects of YK-209 mulberry leaves on antioxidative defense system of liver in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 2002; 31(6): 1065-1070   DOI
59 Chang JH, Kim MS, Kim JY, Choi WH, Lee SS. Effect of mushroom supplementation on blood glucose concentration, lipid profile, and antioxidant enzyme activities in patient with type 2 diabetes mellitus. Korean J Nutr 2007; 40(4): 327-333   과학기술학회마을
60 Duke EJ, Joyce P, Ryan JP. Characterization of alternative molecular forms of xanthine oxidase in the mouse. J Biochem 1973; 131(2): 187-190
61 Lim SJ. Effect of fractions of benincasa hispida on antioxidative status in streptozotocin induced diabetic rats. Korea J Nutr 2007; 40(4): 295-302   과학기술학회마을
62 Rhee SJ, Choe WK, Cha BK, Yang JA, Kim KY. Effect of vitamin E and selenium on the antioxidative defense system in stretozocin-induced diabetic rats. Korean J Nutr 1996; 29(1): 22-31
63 Song YO, Chyun JH. Effect of $\beta$-carotene supplementation on lipid peroxides and antioxidative enzyme activities in hyperlipidemic rats. J Korean Nutr 2004; 39(9): 771-779
64 Mavis RD, Stellwagen E. Purification and subunit structure of glutathione reductase from baker's yeast. J Biological Chemistry 1968; 243(3): 809-814
65 Kwag OC, Yang JA, Rhee SJ. Effects of vitamin E on the antioxidative defense system of kidney in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 1997; 28(3): 651-662
66 El SN, Karakaya S. Radical scavenging and iron-chelating activities of some greens used as traditional dishes in Mediterranean diet. Int J Food Sci Nutr 2004; 55(1): 67-74   DOI   ScienceOn
67 Junod A, Lambert AE, Orci L, Pictet R, Gonet AE, Renold AE. Studies of the diabetogenic action of streptozotocin. Proc Soc Exp Biol Med 1967; 126(1): 201-205
68 Kedziora-Kornatowska K, Luciak M. Effect of arninoguandine on lipid peroxidation and activities of antioxidant enzymes in the diabetics. Biochem Mol Biol Int 1889; 46(3): 557-583