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Effects of Arginine Supplementation on Bone Markers and Hormones in Growing Female Rats  

Choi, Mi-Ja (Department of Food and Nutrition, Keimyung University)
Publication Information
Journal of Nutrition and Health / v.40, no.4, 2007 , pp. 320-326 More about this Journal
Abstract
An important related question is whether arginine has influence bone metabolism. The effect of arginine supplements on bone markers and related hormones were studied in young female Sprague-Dawley rats fed either an arginine supplemented diet or control diet. Twenty four rats (body weight 83${\pm}$5 g) were randomly assigned to one of two groups, consuming casein or casein with supplemented arginine diet. All rats were fed on experimental diet and deionized water ad libitum for 9 weeks. Bone formation was measured by serum osteocalcin and alkaline phosphatase (ALP) concentrations. And bone resorption rate was measured by deoxypyridinoline (DPD) crosslinks immunoassay and corrected for creatinine. Serum osteocalcin, growth hormone, estrogen, insulin-like growth factor-1 (IGF-1), parathyroid hormone (PTH) and calcitonin were analyzed using radioimmunoassay kits. The weight gain and mean food intake were not affected regardless of diets. The rats fed arginine-supplemented diet had not significantly different in ALP, osteocalcin, crosslinks value, PTH, estradiol, and IGF-1 compared to those fed casein diet group. The arginine-supplemented group had significantly higher growth hormone and calcitonin than casein group. This study suggests that arginine is beneficial for bone formation in growing female rats. Therefore exposure to diet which rich in arginine early in life may have benefits for bone formation and osteoporosis prevention.
Keywords
arginine; growth hormone; IGF-1; PTH; bone markers; growing female rat;
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1 Mei J, Yeung SS, Kung AW. High dietary phytoestrogen intake is associated with higher bone mineral density in postmenopausal but not premenopausal women. J Clin Endocrinol Metab 2001; 86(11): 5217-5221   DOI   ScienceOn
2 Komm BS, Terpening CM, Benz DJ, Graeme KA, Gallegos A, Korc M, Greene GL, O'Malley BW, Haussler MR. Estrogen binding, receptor mRNA, and biologic response in osteoblast-like osteosarcoma cells. Science 1998; 241: 81-84
3 Lely AJ. Growth hormone and aging. GH & IGF Res 1999; 9: 117-119   DOI   ScienceOn
4 Tasha LP Ballard, Jeffrey A Clapper, Bonny L Specker, Teresa L Binkley, Matthew D Vukovich. Effect of protein supplementation during a 6-mo strength and conditioning program on insulinlike growth factor I and markers of bone turnover in young adults. Am J Clin Nutr 2005; 81(6): 1442-1448   DOI
5 Krassas GE, Papadopoulou PH, Koliakos G. Growth hormone, insulin growth factor-I, and IGF binding protein-3 axis relationship with bone mineral density among healthy men. Arch Androl 2003; 49: 191-199   DOI   ScienceOn
6 Tran CD, Diorio C, Bérubé S, Pollak M, Brisson J. Relation of insulin-like growth factor (IGF) I and IGF-binding protein 3 concentrations with intakes of fruit, vegetables, and antioxidants. Am J Clin Nutr 2006; 84(6): 1518-1526   DOI
7 Zhang X, Shu XO, Li H, Yang G, Li Q, Gao YT, Zheng W. Prospective cohort study of soy food consumption and risk of bone fracture among postmenopausal women. Archives of Internal Medicine 2005; 165(16): 1890-1895   DOI   ScienceOn
8 Andreassen TT, Jorgensen PH, Flyvbjerg A, Orskov H, Oxlund H. Growth hormone stimulates bone formation and strength of cortical bone in aged rats. J Bone Miner Res 1995; 10: 1057-1067   DOI   ScienceOn
9 Attie KM. The importance of growth hormone replacement therapy for bone mass in young adults with growth hormone deficiency. J Pediatr Endocrinol Metab 2000; 13(suppl): 1011-1021
10 Sugimoto T, Kaji H, Nakaoka D, Yamauchi M, Tano S, Sugishita T, Baylink DJ, Mohan S, Chihara K. Effect of low-dose of recombinant human growth hormone on bone metabolism in elderly women with osteoporosis. Eur J Endocrinol 2002; 147(3): 339-348   DOI   ScienceOn
11 Xing S, Cekan SZ, Dicafalusy U. Validation of radioimunoassay for estadiol-17 isotope dilution-mass spectrometry and a test of radiochemical purity. Clin Chem Acta 1983; 135: 189-201   DOI   ScienceOn
12 Guarnero P, Grimaux M, Seguin P, Delmas P. Characterization of immunoreactive forms of human osteocalcin generated in vivo and in vitro. J Bone Min Res 1994; 9: 692-698
13 Murakami H, Nakamura T, Tsurukami H, Abe M, Barbier A, Suzuke K. Effects of tiludronate on bone mass, structure, and turnover, at the epiphyseal, primary, and secondary spongiosa in the proximal tibia growing rats after sciatic neurectomy. J Bone Miner Res 1994; 9: 1355-1364   DOI   ScienceOn
14 Kalu DN, Masoro EJ, Yu BP, Hardin RR, Hollis BW. Modulation of age related hyperparathyroidism and senile bone loss in fischer rats by soy protein and food restriction. Endocrinology 1998; 122: 1847-1854   DOI   ScienceOn
15 Min HK. Treatment of osteoporosis. J Korean Soc Endocrinol 1989; 4: 1-5
16 Nilsson AG. Effects of growth hormone replacement therapy on bone markers and bone mineral density in growth hormone deficient adults. Horm Res 2000; 54(Suppl 1): 52-57   DOI
17 Choi MJ, Jung SH. The effect of dietary source and sulfur amino acid content on bone metabolism in growing rats. Korean J Nutr 2004; 37(2): 100-107
18 Choi MJ, Cho HJ. Effects of soy and isoflavones on bone metabolism in growing female rats. Korean J Nutr 2003; 36(6): 549-558
19 Choi MJ. Effects of soy and isoflavones on bone markers and hormones in growing male rats. Korean J Nutr 2003; 36(5): 452- 458
20 Ezzat S, Melmed S, Endres D, Eyre DR, Signer FR. Biochemical assessment of bone formation and resorption in acromegaly. J Clin Endocrinol Metab 1993; 76: 1452-1457   DOI   ScienceOn
21 Schurch MA, Rizzoli R, Slosman D, Vadas L, Vergnaud P, Bonjour JP. Protein supplements increase serum insulin-like growth factor-I levels and attenuate proximal femur bone loss in patients with recent hip fracture. Ann Intern Med 1998; 128: 801-809   DOI
22 Lissett CA, Shalet SM. Effects of growth hormone on bone and muscle. Growth Horm IGF Res 2000; 10(Suppl B) ): 95-101   DOI   ScienceOn
23 Mathur SR. Effect of protein source and excercise on skeletal health of growing female rats (dissertation). Texas Woman's University; 1998
24 Barret-Connor E, Goodman-Gruen D. Gender differences in insulin-like growth factor and bone mineral density association in old age: the Rancho Bernardo Study. J Bone Miner Res 1998; 13(8): 1343-1349   DOI   ScienceOn
25 Nanda N, Joshi H, Subbarao SK, Sharma VP. Two-site immunoradiometricassay (IRMA): detection, efficiency, and procedural modifications. J Am Mosq Control Assoc 1994; 10: 225-227
26 Nass R, Pezzoli SS, Chapman IM, Patrie J, Hintz RL, Hartman ML, Thorner MO. IGF-I does not affect the net increase in GH release in response to arginine. Am J Physiol Endocrinol Metab 2002;283(4): E702-710
27 Barnard R, Ng KW, Martin TJ. Growth hormone receptors in clonal osteoblast-like cells mediate a mitogenic response to growth hormone. Endocrinology 1991; 128: 1459-1464   DOI   ScienceOn
28 Knight DC, Eden JA. A review of the clinical effects of phytoestrogens. Obstet Gynecol 1996; 87: 897-904
29 Kerstetter JE, Wall DE, Brien KO, Caseria DM, Insogna KL. Meat and soy protein affect calcium homeostasis in healthy women. J Nutr 2006; 136(7): 1890-1895   DOI
30 Lewinson D, Shenzer P, Hochberg Z. Growth hormone involvement in the regulation of tartrate resistant acid phosphatase positive cells that are active in cartilage and bone resorption. Calcif Tissue Int 1993; 52: 216-221   DOI   ScienceOn
31 Alekel DL, Germain AS, Peterson CT, Hanson KB, Stewart JW, Toda T. Isoflavone-rich soy protein isolate attenuates bone loss in the lumbar spine of perimenopausal women. Am J Clin Nutr 2000; 72:844-852   DOI
32 Monson JP, Arake WM, Carroll PV, Weaver JU, Rodriguez- Arnao J, Savage MO. Influence of growth hormone on accretion of bone mass. Horm Res 2002; 58(Suppl 1): 52s-56s
33 Reeves PG, Nielsen FH, Fahey GC. AIN-93 purified diets for laboratory rodents. J Nutr 1993; 123: 1939-1951   DOI
34 Cowell CT, Woodhead HJ, Broky J. Bone markers and bone mineral density during growth hormone treatment in children with growth hormone deficiency. Horm Res 2000; 54(suppl 1): 44-51
35 Kassem M, Blum W, Risteli J. Growth hormone stimulates proliferation and differentiation of normal human osteoblast-like cells in vitro. Calcif Tissue Int 1993; 52: 222-226   DOI   ScienceOn
36 Kim KL, Kim WY. The effect of soy protein and casein on serum lipid, amino acid. Korean J Nutr 1983; 17: 309-310
37 Gruber HF, Ivey JL, Baylink DJ. Long term calcitonin therapy in postmenopausal osteoporosis. Metabolism 1984; 33: 295-303   DOI   ScienceOn
38 Holmes SJ, Shalet SM. Adult growth hormone deficiency and bone mass. Horm Res 1996; 45(Suppl 1): 69-71   DOI   ScienceOn
39 Hauschka PV, Lian JB, Dole DEC, Gundberg CM. Osteocalcin and matrix gla protein: vitamin K-dependent proteins in bone. Physiol Rev 1989; 69: 990-1047   DOI
40 Ki OH, Kim DY, Yong HI, Woo JT, Kim SW, Yang IM, Kim JW. Changes of bone turnover markers after treatment with growth retardation. J Korea Soc Endocrinol 1994; 9: 344-349
41 Guarnero P, Grimaux M, Seguin P, Delmas P. Characterization of immunoreactive forms of human osteocalcin generated in vivo and in vitro. J Bone Min Res 1994; 9: 692-698
42 Spencer EM, Tobiassen MD. The mechanism of the action of growth hormone on vitamin D metabolism in the rat. Endocrinology 1981; 108: 1064-1070   DOI   ScienceOn
43 Dean HJ, Kellett JG, Bala RM. The effect of growth hormone treatment on somatomedin levels in growth hormone deficient children. J Clin Endocrinol Metab 1982; 55: 1167-1173   DOI
44 Hannon R, Blumsohn A, Dehaimi AW, Eastell R. The use of biochemical markers of bone turnover to monitor the skeletal response to hormone replacement therapy. Bone 1995; 10(suppl 2): 549s-555s
45 Sugimoto T, Nishiyama K, Kuribayashi F, Chihara K. Serum levels of insulin-like growth factor (IGF) I, IGF binding protein (IGFBP)-2 and IGFBP-3 in osteoporotic patients with and without spinal fractures. J Bone Miner Res 1997; 12: 1272-1279   DOI   ScienceOn