Browse > Article

Effects of Arginine Supplementation on Bone Mineral Density in Growing Female Rats  

Choi, Mi-Ja (Department of Food and Nutrition, Keimyung University)
Publication Information
Journal of Nutrition and Health / v.40, no.3, 2007 , pp. 235-241 More about this Journal
Abstract
The aim of this study was to define an arginine effect when added to a diet. The influence of arginine supplements on bone mineral density and content were studied in young female Sprague-Dawley rats fed either an arginine supplemented diet or control diet. Twenty four rats (body weight $83{\pm}5g$) were randomly assigned to one of two groups, consuming casein or casein with supplemented arginine diet. All rats were fed on experimental diet and deionized water ad libitum for 9 weeks. Bone mineral density (BMD) and bone mineral content (BMC) were measured using PIXImus (GE Lunar Co, Wisconsin, USA) in spine and femur 3, 6, and 9 weeks after feeding. The serum and urine concentrations of Ca and P were determined. Diet did not affect weight gain and mean food intake. The serum concentration of Ca and P were not changed by arginine supplementation. Urinary Ca excretion was significantly decreased by arginine supplementation. Spine BMD was significantly increased by arginine supplementation on 3 and 6 weeks after feeding. Femur BMD was significantly increased in the group of arginine supplementation on 3, 6, and 9 weeks. Rats fed the arginine-supplemented diet had better bone mineral content than did control diet rats in the experimental period. Therefore, arginine supplementation may be beneficial on spine and femur BMD increment in growing female rats. These are thought to be associated with an arginine-induced growth hormone release. The exact mechanism of this effect remains to be elucidated.
Keywords
arginine; bone mineral density; growing female rat;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Jayo MJ, Anthony MS, Register C, Rankin SE, Best T, Clarkson TB. Dietary soy isoflavones and bone loss; a study in ovariectomized monkeys. J Bone Mineral Res 1996; 11: s228(Abstr)
2 Song TT, Hendrich S, Murphy PA. Estrogenic activity of glycitein, a soy isoflavone. J Agric Food Chem 1999; 47: 1607-1610   DOI   ScienceOn
3 Dwyer JT, Goldin BR, Saul N, Gualtieri L, Barakat S, Adlercreutz H. Tofu and soy drinks contain phytoestrogens. J Am Diet Assoc 1994; 94: 739-743   DOI   ScienceOn
4 Andreassen TT, Jorgensen PH, Flyvbjerg A, Orskov H, Oxlund H. Growth hormone stimulates bone formation and strength of cortical bone in aged rats. J Bone Miner Res 1995; 10: 1057-1067   DOI   ScienceOn
5 Reeves PG, Nielsen FH, Fahey GC. 1993. AIN-93 purified diets for laboratory rodents. J Nutr 1993; 123: 1939-1951   DOI
6 Daly JA, Ertings HG. Direct method for determine inorganic phosphate in serum with the centrifichem. Clin Chem 1972; 18: 263-265
7 Choi MJ, Jung SH. The effects of dietary protein source and sulfur amino acid content on bone metabolism in growing rats. Korean J Nutr 2004; 37(2): 100-107
8 Whiting SJ, McNally ME. Calciuric effect of diets high in soy, beef or lactoalbumin protein in the rat. Nut Rep Intl 1989; 40: 199-205
9 Im SK, Jung HC, Lee MK, Kim HM, Lee HC, Hu GB. Risk factor of osteopenia in Korean women. J Korean Soc Internal Med 1976; 34: 444-451
10 Kang MI. Editorial: Prevalence and risk factors of the osteoporosis of perimenopausal women. Kor Med J 2002; 62: 103
11 Zubik L, Meydani M. Bioavailability of soybean isoflavones from aglycone and glucoside forms in American women. Am J Clin Nutr 2003; 77(6): 1459-1465   DOI
12 Atkinson C, Compston JE, Day NE, Dowsett M, Bingham SA. The effects of phytoestrogen isoflavones on bone density in women: a double-blind, randomized, placebo-controlled trial. Am J Clin Nutr 2004; 79(2): 326-333   DOI
13 Funaba M, Kawashima T, Yano H, Kawashima R. Effects of a high protein diet on bone formation and calcium metabolism in rats. J Nutr Sci Vitaminol 1990; 36: 559-567   DOI
14 Choi MJ. Jo HJ. Effects of soy protein and isoflavones on bone mineral density in growing female rats. Korean J Nutr 2003; 36 (4): 359-367
15 Nass R, Pezzoli SS, Chapman IM, Patrie J, Hintz RL, Hartman ML, Thorner MO. IGF-I does not affect the net increase in GH release in response to arginine. Am J Physiol Endocrinol Metab 2002; 283(4): E702-710
16 Calvo MS, Bell RR, Forbes RM. Effect of protein-induced calciuria on calcium metabolism and bone status in adult rats. J Nutr 1982; 112: 1401-1409   DOI
17 Mituka BM, Rawnsley HN. Clinical biochemical and hematologycal reference value in normal experimental animals and normal humans. 2nd edition, Masson, New York; 1987. p.160
18 Potter SM, Baum JA, Teng H, Stillman RJ, Shay NF, Erdman JW Jr. Soy protein and isoflavones: their effects on blood lipids and bone density in postmenopausal women. Am J Clin Nutr 1998; 68(suppl): 1375S-1379S
19 Dean HJ, Kellett JG, Bala RM. The effect of growth hormone treatment on somatomedin levels in growth hormone deficient children. J Clin Endocrinol Metab 1982; 55: 1167-1173   DOI
20 Ho SC. Soy intake and the maintenance of peak bone mass in Hong Kong Chinese women. J Bone Miner Res 2001; 16: 1363-1369   DOI   ScienceOn
21 Schuette SA, Hegsted M, Zemel MB, Linkswiler HM. Renal acid, urinary cyclic AMP, and hydroxyproline excretion as affected by level of protein, sulfur amino acid, and phosphorus intake. J Nutr 1981; 111: 2106-2116   DOI
22 Khalil DA, Lucas EA, Juma S, Smith BJ, Payton ME, Arjmandi BH. Soy protein supplementation increases serum insulin-like growth factor-I in young and old men but does not affect markers of bone metabolism. J Nutr 2002; 132(9): 2605-2608   DOI
23 Rico H, Amo C, Revilla M, Arribas I, Gonzales-Rida J, Villa LF, Rodriguez-Puyol M. Etidrinate versus Clodronate in the prevention of postovariectomy bone loss: An experimental study in rats. An experimental study in rats. Clin Exp Rheumatol 1994; 12: 301-304
24 Dalais FS, Rice GE, Wahlqvist ML, Grehan M, Murkies AL, Medley G, Ayton R, Strauss BJ. Effects of dietary phytoestrogens in postmenopausal women. Climacteric 1998; 1: 124-129   DOI   ScienceOn
25 Arjmandi BH, Birnbaum R, Goyal NV, Getlinger MJ, Juma S, Alekel L, Hasler CM, Drum ML, Hollos BW, Kukreja SC. Bonesparing effect of soy protein in ovarian hormone-deficient rats is related to its isoflavone content. Am J Clin Nutr 1998; 68: 1364-1368   DOI
26 Steinberg FM, Guthrie NL, Villablanca AC, Kumar K, Murray MJ. Soy protein with isoflavones has favorable effects on endothelial function that are independent of lipid and antioxidant effects in healthy postmenopausal women. Am J Clin Nutr 2003; 78(1): 123-130   DOI
27 Arjmandi BH, Alekel L, Hollis BW. Dietary soybean protein prevents bone loss in an ovariectomized osteoporosis. J Nutr 1996; 126: 161-167   DOI
28 Newsholme EA, Leech AR. Biochemistry for the medical sciences. New York: Wiley & Sons, 1983. Windmueller HG, Spaeth AE. Source and fate of circulating citrulline. Am J Physiol 1981; 241: E473-480
29 Civitelli R, Gonnelli S, Zacchei F. Bone turnover in postmenopausal osteoporosis: 12 year study. Br Med J 303: 961-964, 1991   DOI   ScienceOn
30 Kim KL, Kim WY. The effect of soy protein and casein on serum lipid, amino acid. Korean J Nutr 1983; 17: 309-310
31 Zak B, Epstein E, Baginski ES. Aphthalein responding to alkaline earth ions and analytical applications. Ann Clin Laboratory Sci 1975; 5: 195-215
32 Attie KM. The importance of growth hormone replacement therapy for bone mass in young adults with growth hormone deficiency. J Pediatr Endocrinol Metab 2000; 13(suppl): 1011-1021
33 Zemel MB. Calcium utilization: effect of varying level and source of dietary protein. Am J Clin Nutr 1988; 48(3): 880s-883s
34 Choi MJ. Effects of soy protein on bone mineral content and bone mineral density in growing male rats. Korean J Nutr 2002; 35(4): 409-413
35 Heys SD, Gardner E. Nutrients and the surgical patient: current and potential therapeutic applications to clinical practice. J R Coll Surg Edinb 1999; 44: 283-293
36 Choi MG, Lee YS, Sung JJ. Effects of Caffeine Intake on Calcium Utilization in Rats of Different Age and Sex. Korean J Nutr 1997; 30(8): 911-919
37 Monson JP, Arake WM, Carroll PV, Weaver JU, Rodriguez-Arnao J, Savage MO. Influence of growth hormone on accretion of bone mass. Horm Res 2002; 58(Suppl)1: 52s-56s
38 Cooper C, Campion G, Melton LJ. Hip fractures in the elderly: a world-wide projection. Osteoporosis Int 1992; 2: 285-289   DOI   ScienceOn
39 Linkswiler HM, Zemel MB, Hegsted M, Schuette S. Protein-induced hypercalciuria. Fed Proc 1981; 40: 2429-2433
40 Setchell KDR, Borriello SP, Hulme P, Axelson M. Nonsteroidal estrogens of dietary origin: Possible roles in hormone dependent disease. Am J Clin Nutr 1984; 40: 569-578   DOI
41 Choi MJ, Jo HJ. Effects of Soy and Isoflavones on Bone Metabolism in Growing Female Rats. Korean J Nutr 2003; 36(6): 549-558