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Effect of Dietary Fat and Genistein on Lipid Metabolism and Antioxidant Activity in Hyperlipidemic Male Rats induced High Fat Diet  

Kim Mi-Hyun (Department of Food & Nutrition, Research Institute of Human Ecology, Seoul National University)
Jang So-Young (Department of Food & Nutrition, Research Institute of Human Ecology, Seoul National University)
Lee Yeon-Sook (Department of Food & Nutrition, Research Institute of Human Ecology, Seoul National University)
Publication Information
Journal of Nutrition and Health / v.39, no.2, 2006 , pp. 100-108 More about this Journal
Abstract
This study was conducted to investigate whether dietary factors, normal fat and genistein leads to beneficial improvement of lipid metabolism and oxidative stress in adult hyperlipidemic male rats. Seven wk-old male SD rats were fed high fat diet (15% fat, 1% cholesterol) for 4 wks for induction of hyperlipidemic model rat. Weight-matched rats were then assigned to four groups according to dietary fat level (7% or 15% fat) and genistein contents (0 or 320 mg/kg diet). Food intake was significantly decreased by both high fat intake and genistein supplementation compared with normal fat intake and genistein no supplementaion. But weight gain was significantly decreased by genistein supplementation in normal fat intake compared with the other groups. Total lipid, total cholesterol and triglyceride in serum and liver were significantly decreased by normal fat intake compared with high fat intake. But total cholesterol in liver was significantly increased by genistein supplementation in both high fat and normal fat intake. TBARS in serum and liver was less produced by normal fat intake compared with high fat intake but TBARS in liver was significantly increased by genistein supplementation compared with genistein no supplementation in normal fat intake. Glutathione reductase activity in erythrocytes was significantly reduced by genistein supplementation in normal fat intake compared with the other groups. Glutathione peroxidase and glutathione reductase activities in liver were significantly inhibited by normal fat intake compared with high fat intake. Catalase activity in liver was significantly increased by genistein supplementation compared with genistein no supplementation in high fat intake. Nitrite was significantly decreased by normal fat intake compared with high fat intake. These results suggest that normal fat intake has the treatment effect against risk factors related with cardiovascular disease by reducing lipid profiles, lipid peroxidation. And genistein shows action as a antioxidant replacing antioxidant enzymes but also may act as prooxidant causing the production of TBARS.
Keywords
fat and genistein; lipid profiles; antioxidant enzyme activities; TBARS; nitrite;
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1 Chahoud G, Aude YW, Metha JL. Dietaray recommendations in the prevention and treatment of coronary heart disease: do we have the ideal diet yet? Am J Cardiol 94: 1260-1267, 2004   DOI   ScienceOn
2 McNeel RL, Mersmann HJ. Low-and high-carbohyrate diets: body composition differences in rats. Obes Res 13(10): 1651- 1660, 2005   DOI   ScienceOn
3 Shah M, Garg A. High-fat and high-carbohydrate diets and energy balance. Diabetes Care 19(10) : 1142-1152, 1996   DOI   ScienceOn
4 Kim MS, Lee YS. The effect of isoflavone and/or estrogen treatments on bone metabolism in the ovariectomized rats. J Med Food 8: 439-445, 2005   DOI   ScienceOn
5 Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37: 911-917, 1959   DOI
6 Melanson KJ, Westerterp-Plantenga MS, Saris WHM, Campfield LA. Blood glucose patterns and appetite in time-blinded humans: carbohydrate versus fat. Regul Integr Comp Physiol 46: R337- 345, 1999
7 Ahotupa M, Bereziat JC, Mantyla E, Bartsch H. Dietary fat-and phenolbarbital-induced alternations in hepatic antioxidant functions of mice. Carcinogenesis 14(6): 1225-1228, 1993   DOI   ScienceOn
8 Wang Y, Jones PJ, Ausman LM, Lichtenstein AH. Soy protein reduces triglyceride levels and triglyceride fatty acid fractional synthesis rate in hypercholesterolemic subjects. Atherosclerosis 173(2): 269-275, 2004   DOI   ScienceOn
9 Statistics of Death Cause, Korea National Statistical Office, Seoul, 2004
10 Frings CS, Dunn RT. The colorimetric method for determination of serum total lipids based on the sulfo-phosphovanilin reaction. Am J Clin Pathol 52: 89-91, 1970
11 Nam JH, Park HS. Effect of quality and quantity of dietary fat on status of tocopherol and lipid peroxidation of plasma and tissue in rats. Korean J Nutrition 26(5): 566-577, 1993   과학기술학회마을
12 Yamakoshi J, Piskula MK, Izumi T, Tobe K, Saito M, Kataoka S, Obata A, Kikuchi M. Isoflavone aglycon-rich extract without soy protein attenuates atherosclerosis development in cholesterol fed rabbits. J Nutr 130: 1887-1893, 2000
13 Westerterp-Plantenga MS, Kovacs EMR, Melanson KJ. Habitual meal frequency and energy intake regulation in partially temporally isolated men. Int J Obes Relat Metab Disord 26: 102-110, 2002   DOI   ScienceOn
14 Lee YS, Jang SY, Kim KO. Effect of soy isoflavone intake on nitrite content and antioxidant enzyme activities in male rats fed high-fat diet. Korean J Nutrition 38(2): 89-95, 2005   과학기술학회마을
15 Friedwald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low density lipoprotein cholesterol in plasma without use of the preparative ultracentrifugation. Clin Chem 18: 499- 502, 1972
16 Hiramoto K, Ohkawa T, Oikawa N, Kikugawa K. In nitric oxide (NO) an antioxidant or a prooxidant for lipid peroxidations? Hem Pharm Bull 51: 1046-1050, 2003   DOI   ScienceOn
17 Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Exzymol 52: 302-310, 1978   DOI
18 Barnard ND, Scialli AR, Bertron P, Hurlock D, Edmonds K, Talev L. Effectiveness of a low-fat vegetarian diet in altering serum lipids in healthy premenopausal women. Am J Cardiol 85: 969-972, 2000   DOI   ScienceOn
19 Tappel AL. Glutathione peroxidase and hydroperoxides. Methods Enzymol 52: 506-513, 1978   DOI
20 Cardiovascular disease risk factors: new areas of research, World Health Organization, Geneva, 1994
21 Patel RP, Boersma BJ, Crawford JH, Hogg N, Kirk M, Kalyanaraman B, Parks DA, Barnes S, Darley-Usmar V. Antioxidant mechanis of isoflavones in lipid systems: paradoxical effects of peroxyl radical scavenging. Free Radic Biol Med 31(12): 1570- 1581, 2001   DOI   ScienceOn
22 Rahman MM, Varghese Z, Moorhead JF. Paradoxical increase in nitric oxide synthase activity in hypercholesterolaemic rats with impaired renal function and decreased activity of nitric oxide. Nephrol Dial Transplant 16: 262-268, 2001   DOI   ScienceOn
23 Bocehnek W, Rodges JB. Effect of saturated and unsaturated fats give with and without cholesterol on hepatic cholesterol synthesis and hepatic lipid metabolism. Biochem Biophys Acta 528: 1, 1978
24 Kyselova V, Peknicova J, Boubelik M, Buckiova D. Body and organ weight, sperm acrosomal status and reproduction after genistein and diethylstilbestrol treatment of CD1 mice in a multigenerational study. Theriogenology 61(7): 1307-1325, 2004   DOI   ScienceOn
25 Roberts CK, Vaziri ND, Wang XQ, Barnard RJ. Enhanced NO inactivation and hypertension induced by a high-fat, refinedcarbohydrate diet. Hypertension 36: 423-429, 2000   DOI
26 Aebi H. Catalase in vitro. Methods Enzymol 105: 121-126, 1984   DOI
27 Frantz ID, Jr, Dawson EA, Ashman PL, Gatewood LC, Bartsh GE, Kuba K, Brewer ER. Test of the effect of lipid lowering by diet on cardiovascular risk. The Minnesota Coronary Survey. Atherosclerosis 9: 129-135, 1989
28 Griess P. Bemerkungen zu der abhandlung der H. H. Weselsky un Benedikt Ueber einige azoverbindungen. Chem Ber 12: 426, 1879   DOI
29 Goodman-Gruen D, Kritz-Silverstein D. Usual dietary isoflavone intake and body composition in postmenopausal women. Menopause 10(5): 427-432, 2003   DOI   ScienceOn
30 Giugliano D. Dietary antioxidants for cardiovascular prevention. Nutr Metab Cardiovasc Dis 10(1): 38-44, 2000
31 Park SN. Protective effect of isoflavone, genistein from soybean on singlet oxygen induced photohemolysis of human erythrocytes. Korean J Food Sci Technol 35(3): 510-518, 2003   과학기술학회마을
32 Carlberg I, Mannervik B. Glutathione reductase. Methods Enzymol 113: 484-490, 1985   DOI
33 Kawakami Y, Tsurugasaki W, Yoshida Y, Igarashi Y, Nakamura S, Osada K. Regulative actions of dietary soy isoflavone on biological antioxidative system and lipid metabolism in rats. J Agric Food Chem 52(6): 1764-1768, 2004   DOI   ScienceOn
34 Kirk EA, Sutherland P, Wang SA, Chait A, Le boeuf RC. Dietary isoflavones reduces plasma cholesterol and atherosclerosis in C57BL/6 mice but not LDL receptor-deficient mice. J Nutr 128: 954-959, 1998
35 Squadrito F, Altavilla D, Morabito N, Crisafulli A, D'Anna R, Corrado F, Ruggeri P, Campo GM, Calapai G, Caputi AP, Squadrito G. The effect of the phytoestrogen genistein on plasma nitric oxide concentrations, endothelin-1 levels and endothelium dependent vailation in postmenopausal women. Atherosclerosis 163: 339-347, 2002   DOI   ScienceOn
36 Abbott WGH, Swinbum B, Ruotolo G, Hara H, Patt Li, Harper I, Grundy SM, Howard BV. Effect of a high-carbohydrate, lowsaturated-fat diet on apolipoprotein B and triglyceride metabolism in pima iadians. J Clin Invest 86: 642-650, 1990   DOI   ScienceOn