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Effect of DHEA Administration on PUFA/SFA Ratio and Lipid Peroxide in Rat Liver Microsome  

Kwak Chune Shil (Aging and Physical Culture Research Institute, Seoul National University)
Kim Mee Yeon (Department of Food Science and Human Nutrition, Ewha Woman's University)
Publication Information
Journal of Nutrition and Health / v.38, no.4, 2005 , pp. 297-306 More about this Journal
Abstract
It is known that dehydroepiandrosterone (DHEA) shows a dual effect, prooxidant or antioxidant, depending on the do-sage or physiological status of animals. The purpose of this study was to determine the effects of DHEA administration at low dose on lipid peroxidation, protein carbonylation and fatty acid composition in liver. Sprague Dawley male rats were fed either com oil diet containing $15\%$ com oil or fish oil diet containing $2\%$ corn oil + $13\%$ sardine oil, with or without $0.2\%$ DHEA for 9 weeks. Atherogenic index and hepatic triglyceride and cholesterol levels were significantly reduced by DHEA administration in rats fed with fish oil diet. Hepatic lipid peroxide product (TBARS) and protein carbonyl levels were significantly higher in rats fed with fish oil diet than in rats fed with corn oil diet. However, DHEA administration significantly reduced the hepatic thiobarbituric acid-reactive substance (TBARS) and conjugated diene levels in rats fed with fish oil diet. Contents of C16 : 0, C16 : 1, C20 : 5 and C22 : 6 in hepatic microsome were higher in rats fed with fish oil diet than in rats fed with corn oil diet, and contents of C18 : 2 and C20 : 4 were lower than in rats fed with com oil diet. DHEA administration significantly increased C16 : 0 and C18 : 3 contents and reduced C18 : 2 content in rats fed with com oil diet, while it increased C16 : 0 and C18 : 1 and reduced C20 : 5 and C22 : 6 in rats fed with fish oil diet. On overall, DHEA administration increased saturated fatty acid (SFA) and reduced polyunsaturated fatty acid (PUFA) in hepatic microsome, thereby PUFA/SFA ratio was significantly (p < 0.0001) reduced without the change of n-3/n-6 ratio. Taken together, low dose of DHEA administration lowered PUFA/SFA ratio in hepatic microsomal membranes and also showed antioxidative effect especially in fish oil-induced highly oxidative stress condition through blocking increases of C20 : 5 and C22 : 6 contents.
Keywords
dehydroepiandrosterone; fatty acid composition; hepatic microsome; fish oil; antioxidative effect;
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1 McIntosh MK, Berdainer CD. Antiobesity effects of dehydroepiandrosterone are mediated by futile substrate cycling in hepatocytes of BHE/cdb rats. J Nutr 121: 2037-2043, 1991
2 Kwak CS, Park SC. Oxidative stress and peroxisomal proliferation induced by short-term administration of DHEA and fish oil in rat liver tissue. Korean J Gerontology 8 (2): 134-148, 1998
3 Aragno M, Brignardello E, Tamagno E, Gatto V, Danni O, Boccuzzi G. Dehydroepiandrosterone administration prevents the oxidative damage by acute hyperglycemia in rats. J Endocrinology 155: 233-240, 1997   DOI   ScienceOn
4 Mohan PF, Cleary MP. Short-term effects of dehydroepiandrosterone treatment in rats on mitochondrial respiration. J Nutr 121: 240-250,1991
5 Aragno M, Parola S, Brignardello E, Mauro A, Tamagno E, Manti R, Danni O, Boccuzzi G. Dehydroepiandrosterone prevents oxidatively injury induced by transient ischemialreperfusion in the brain of diabetic rats. Diabetes 49: 1924-1931, 2000   DOI   ScienceOn
6 Uchiyama M, Mihara M. Determination of malondialdehyde procedure in tissue by thiobarbituric acid test. Anal Biochem 86: 271-278, 1978   DOI   ScienceOn
7 Chevalier M, Lin EC, Levine RL. Hydrogen peroxide mediates the oxidative inactivation of enzymes following the switch from anaerobic to aerobic metabolism in Klebsiella pneumoniae. J Biol Chem 265: 42-46, 1983
8 Stadtman ER. Metal ion catalyzed oxidation of proteins: Biochemical mechanism and biological consequences. Free Rad Biol Med 9: 315-325, 1990   DOI   ScienceOn
9 Abadie JM, Malcom GT, Porter JR, Svec F. Dehydroepiandrosterone alters lipid profiles in Zucker rats. Lipids 35 (6) : 613-620, 2000   DOI   ScienceOn
10 Coleman DL, Schwizer RW, Leiter EH. Effect of genetic background on the therapeutic effects of dehydroepiandrosterone in diabetes-obesity mutants and in aged normal mice. Diabetes 33: 26-32,1984   DOI   ScienceOn
11 Miller BC, Lau HW, Tyler NE, Cotan GL. Liver composition and lipid metabolism in NZB/WF1 female mice fed dehydroepiandrosterone. Biochim Biophys Acta 962: 25-36, 1988
12 Sola R, La Ville AE, Richard JL, Motta C, Bargallo MT, Girona J, Masana L, Jacotot B. Oleic acid rich diet protect against the oxidative modification of high density lipoprotein. Free Radic Biol Med 22(6): 1037-1045, 1997   DOI   ScienceOn
13 Aragno M, Tamagno E, Buccuzzi G, Brignardello E, Chiarpotto E, Pizzini A, Danni O. Dehydroepiandrosterone pretreatment protects rats against the pro-oxidant and necrogenic effects of carbon teteachloride. Biochem PharmacoI 46(10): 1689-1694,1993   DOI   ScienceOn
14 Boccuzzi G, Aragno M, Seccia M, Brignardello E, Tamagno E, Albano E, Danni O, Bellomo G. Protective effect of dehydroepiandrosterone against copper-induced lipid peroxidation in the rat. Free Radical Bioi Med 22(7): 1289-1294,1997   DOI   ScienceOn
15 Gallo M, Aragno M, Gatto V,Tamagno E, Brignardello E, Manti R, Danni O, Boccuzzi G. Protective effect of dehydroepiandrosterone against lipid peroxidation in a human liver cell line. Eur J Endocrinology 141: 35-39, 1999   DOI   ScienceOn
16 Lea-Currie YR, Wen P, McIntosh MK. Dehydroepiandrosterone-sulfate reduces adipocyte hyperplasia associated with feeding rats a high-fat diet.Inter J Obesity 21: 1058-1064, 1997   DOI
17 Swierczynski J, Bannasch P, Mayer D. Increase of lipid peroxidation in rat liver microsomes by dehydroepiandrosterone feeding. Biochim Biophys Acta 1315: 193-198, 1996
18 Omundsen H, Bjornstad K. Inhibitory effects of some long-chain unsaturated fatty acids on mitochondrial .$\beta$-oxidation. Biochem J 230:329-337, 1985
19 Regelson W, Kalimi M. DHEA-the multifunctional steroid. II. Effects on the CNS, cell proliferation, metabolic and vascular, clinical and other effects. Mechanism of action. Ann NY Acad Sci 719: 564-576, 1994   DOI   PUBMED
20 Mohan PF, Phillips FC, Cleary MP. Metabolic effects of coconut, safflower, or menhaden oil feeding in lean and obese Zucker rats. Br J Nutr 66 (2) : 285-299, 1991   DOI   ScienceOn
21 Rao MS, Reddy JK. An overview of peroxisome proliferatorinduced hepatocarcinogenesis. Envirn Health Perspectives 93: 205-209, 1991   DOI   PUBMED
22 Bradford M. A rapid and method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 240-254, 1970
23 Richards RJ, Porter J, Svec F. Serum leptin, lipids, free fatty acids and fat pads in long-term dehydroepiandrosterone-treated Zucker rats. PSEBM 223: 258-262, 2000   DOI   ScienceOn
24 Marrero M, Prough RA, Frenkel RA, Milewich L. Dehydroepiandrosterone feeding and protein phosphorylation, phosphatases, and lipogenic enzymes in mouse liver. Proc Soc Exp Biol Med 193 (2) : 110-117, 1990
25 Lepage G, Roy CC. Direct transesterification of lipids in a onestep reaction. J Lipid Res 27: 114-120,1986
26 Imai K, Koyama M, Kudo N, Shirahata A, Kawashima Y. Increase in hepatic content of oleic acid induced by dehydroepiandrosterone in the rat. Biochem Phormacology 58: 925-933, 1999   DOI   PUBMED   ScienceOn
27 Morales AJ, Nolan JJ, Nelson JC, Yen SS. Effects of replacement dose of dehydroepiandrosterone in men and women of advancing age. J Clin Endocr Metab 78: 1360-1367, 1994   DOI
28 Swierczynski J, Mayer D. Vitamin E prevents induction of carbonyl group formation in microsomal protein by dehydroepiandrosterone. Nutr Cancer 32 (2): 10 1-1 06, 1998   DOI   ScienceOn
29 Hornsby PJ. Biosynthesis of DHEAS by the human adrenal cortex and its age-related decline. Ann NY Acad Sci 774: 29-46, 1995   DOI   PUBMED
30 Milewich L. Catalina F, Bennett M. Pleotropic effects of dietary DHEA. In: Dehydroepiandrosterone (DHEA) and aging. Ann NY Acad Sci 774: 149-170,1995   DOI   PUBMED
31 Levine RL, Carland D, Oliver CN, Anici A, Aliment I, Lens AG, Ahn BW, Shaltiel S, Stadtrnann ER. Determination of carbonyl content in oxidatively modified protein. Methods in Enzymology, William BJ Ed. Academic Press Inc NY 186: 464-478, 1990
32 Falany CN, Corner KA, Dooley TP, Glatte H. Human DHEA sulfotransferase. In: Dehydroepiandrosterone (DHEA) and aging. Annal NY Aca Sci 774: 59-71, 1995   DOI   PUBMED
33 Bednarek-Tupikowska G, Gosk I, Szuba A, Bohdanowicz-Pawlak A, Kosowaka B, Bidzinska B, Milewiez A. Influence of dehydroepiandrosterone on platelet aggregation, superoxide dismutase activity and serum lipid peroxide concentrations in rabbits with induced hypercholesterolemia. Med Sci Monit 6: 40-45, 2000
34 Aragno M, Tamagno E, Gatto V, Brignardello E, Parola S, Danni O, Boccuzzi G. Dehydroepiandrosterone protects tissues of streptozotocin-treated rats against oxidative stress. Free radic Biol Med 26: 1467-1474, 1999   DOI   ScienceOn
35 Bayindir 0, Ozmen D, Mutaf I, Turgan N, Habif S, Gulter C, Parildar Z, Uysal A. Comparision of the effects of dietary saturated, mono-, and n-6 polyunsaturated fatty acids on blood lipid profile, oxidant stress, prostanoid synthesis and aortic histology in rabbits. Ann Nutr Metab 46 (5) : 222-228, 2002   DOI   ScienceOn
36 Malarkey WB, Hall JC, Rice RR, O'Toole ML, Douglas PS, Demers LM, Glaser R. The influence of age on endocrine responses to ultraendurance stress. J Gerontol 48: M134-M139, 1993
37 Prough RA, Webb SJ, Wu HQ, Lapenson DP, Waxman DJ. Induction of microsomal and peroxisomal enzymes by dehydroepiandrosterone and its reduced metabolite in rats. Cancer Res 54: 2878-2886, 1994
38 Wu HQ, Masset-Brown J, Tweedie DJ, Melewich L, Frenkel RA, Martin-Wixtrom C, Estabrook RW, Prough RA. Introduction of microsomal NADPH-cytochrome P450 reduced and cytochrome P450IVI (P450LA $\omega$) by dehydroepiandrosterone: a possible peroxisomal proliferator. Cancer Res 49: 2337-2343, 1989
39 Mastrocola A, Aragno M, Betteto S, Brignardello E, Catalano MG, Danni O, Boccuzzi G. Pro-oxidant effect of dehydroepiandrosterone in rats is mediated by PPAR activation. Life Sci 73: 289-299, 2003   DOI   ScienceOn
40 Aragno M, Mastrocola R, Brignardello E, Catalano M, Robino G, Manti R, Parola M, Danni O, Boccuzzi G. Dehydroepiandrosterone modulates nuclear factor- $\kappa$ B activation in hippocampus of diabetic rats. Endocrinology 143: 3250-3258,2002   DOI   ScienceOn
41 Kurzman ID, MacEwen EG, Haffa ALM. Reduction in body weight and cholesterol in spontaneously obese by DHEA. Int J Obesity 14: 95-104, 1990
42 Rechnagel RO, Glende EAJ. Spectrophotometric detection of lipid conjugated dienes. Method Enzymol 105: 331-337, 1984   DOI
43 Tamagno E, Aragno M, Boccuzzi G, Gallo M, Parola S, Fubini B, Poli G, Danni O. Oxygen free radical scavenger properties of dehydroepiandrosterone. Biochem Funct 16(1): 57-63, 1998   DOI   ScienceOn
44 Bellei M, Battelli D, Fornieri C, Mori G, Muscatello U, Lardy H, Bobyleva V. Changes in liver structure and function after shortterm and long-term treatment of rats with DHEA. J Nutr 122: 967-976,1992
45 Folch J, Lees M, Stanley GHS. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 497-509, 1957
46 Perona JS, Arcemis C, Ruiz-Gutierrez V, Catala A. Effect of dietary high-oleic-acid that are rich in antioxidants on microsomal lipid peroxidation in rats. J Agric Food Chern 53: 730-735,2005   DOI   ScienceOn
47 Nestler JE, Barlascini CD, Clore JN, Blackard WG. DHEA reduces serum low lipoprotein levels and body fat but dose not alter insulin sensitivity in normal men. J Clin Endo Metab 66: 57-65,1988   DOI