Browse > Article

Effects of Dietary Levels of Corn and Tuna Oils on the Formation of Preneoplastic Lesions in Rat Hepatocellular Carcinogenesis  

Kim Sook hee (혜전대학 호텔조리계열)
Kang Sang kyoung (서울대학교 생활과학대학 식품영양학과)
Choi Hay mie (서울대학교 생활과학대학 식품영양학과)
Publication Information
Journal of Nutrition and Health / v.38, no.1, 2005 , pp. 20-29 More about this Journal
Abstract
This study is conducted to determine the effects of dietary levels of corn and tuna oils on the formation of preneoplastic lesions in die-thylnitrosamine (DEN) induced rat hepatocarcinogenesis. Weanling male Sprague-Dawley rats were fed 2.5, 5, 15, 25% (w/w) corn or tuna oils. Hepatocellular carcinogenesis was induced by DEN (200 mg/kg body weight) and two-thirds partial hepactectomy was carried out 3 weeks later and were sacrificed 8 weeks after DEN initiation. Tuna oil group showed smaller area of placental glutathione S-transferase (GST-P) positive foci than com oil group. Com oil group of 25% (w/w) showed the widest area of GST -P positive foci, and tuna oil group showed significantly smaller area of GST-P positive foci than com oil in 25% (w/w) level but had no differences between oil levels. Thio-barbituric acid reactive substances (TBARS) content was the highest in 25% (w/w) level of tuna oil group fed long chain and highly polyunsaturated fatty acids. Also serum ${\gamma}$ -glutamyltranspeptidase (GGT) activities in 25% level of tuna oil group were significantly higher than by other levels. As oil contents increased, glucose 6-phosphatase (G6Pase) seems to decrease in com oil groups but remained the same in tuna oil groups. Glutathione reductase (GR) activities were significantly higher in tuna oil group, and the higher the level of tuna oil, the higher GR activities. But Cu/Zn superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities didn't seem to be influenced by levels and kind of dietary fats. Therefore, as oil levels increased, com oil rich in n-6 fatty acids promoted carcinogenesis but tuna oil rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) of n-3 fatty acids suppressed. Although lipid peroxidation products were elevated in 25% (w/w) tuna oil group, GST-P positive foci didn't increase. Therefore pre-neoplastic lesions might be reduced through mediation of a lipid peroxidation process in tuna oil. As fat contents of tuna oil increased, elevated GR activities may give a rise to produce more reduced glutathione in order to protect against free radical attack, and high G6Pase activities remained the same and they contributed to membrane stability. So tuna oil diet seems to protect hepatocarcinogenesis.
Keywords
corn oil; tuna oil; hepatocarcinogenesis; GST-P positive foci;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Prentice RL, Thompson D, Clifford C, Gorbach S, Golden B, Byar D. Dietary fat reduction and plasma estradiol concentration in healthy postmenopausal women. J Natl Cancer Inst 82 (2): 129-134, 1990   DOI   PUBMED
2 Howard PG, Travis L, Wendy SK, Henry CP. Effect of dietary fat on the initiation of hepatocarcinogenesis by diethylnitrosamine or 2-acetylaminofluorene in rats. Carcinogenesis 12: 991-995, 1991   DOI   ScienceOn
3 Blot WJ, Lanier A, Fraumeni JF, Jr, Bender TR. Cancer mortality among Alaska natives, 1960-69. J Natl Cancer Inst 55: 546-554, 1975
4 Roebuck BD, Longnecker DS, Baumgartner KJ, Thron CD. Carcinogen induced Lesions in rat pancreas: Effect of Varying levels of Essential Fatty acid. Cancer Res 45: 5252-5256, 1985
5 Ito N, Tsuda H, Tatematsu M, Inoue T, Tagawa Y, Aoki T, Uwagawa S, Kagawa M, Ogiso T, Masui T. Enhancing effect of various hepatocarcinogens on induction of preneoplastic glutathione S-transferase placental form positive foci in rats-an approach for a new medium-term bioassay system. Carcinogenesis 9(3): 387-394, 1988   DOI   ScienceOn
6 Vaca CE, Harms-Ringdahl M. Lipid peroxidation on the rat liver S9 fraction: Influence of membrane lipid composition. Mutation Res 162: 21-32, 1986
7 Carlberg I, Mannervick B. Glutathione reductase. Methods Enzymology 113:484-499, 1985   DOI
8 Sato K, Kitahara A, Sato K, Ishikawa T, Tatematusu M, Ito N. The placental form of glutathione S-transferase as a new marker protein for preneoplasia in rat chemical hepatocarcinogenesis. Jpn J Cancer Res 75: 199-202, 1984
9 Ahotupa M, Bereziat JC, Mantyla E, Bartsch H. Dietary fat- and phanobarbital-induced alteration in hepatic antioxidant functions of mice. Carcinogenesis 14: 1225-1228, 1993   DOI   ScienceOn
10 Glauret HP, Lay JT, Kennan WS, Pitot HC. Effect of dietary fat on the initiation of hepatocarcinogenesis by diethylnitrosarnine or 2-acetylarninofluorene in rats. Carcinogenesis 12: 991-995, 1991   DOI   ScienceOn
11 Woo J. Effects of different dietary fats and carcinogen treatment on the hepatic microsomal drug metabolizing enzymes in hepatocellular carcinogenesis. Master's thesis of Seoul National University, 1995
12 Minoura T, Takata T, Sakaguchi M. Effect of dietary eicosapentaenoic acid on azomethane-induced colon carcinogenesis in rats. Cancer Res 48: 4790-4794, 1988
13 Kvannes J, Ekihom TS, Flatmark T. On the mechanism of stimulation of peroxisomal ${\beta}$-oxidation in rat heart by partially hydrogenated fish oil. BBA 1255: 39-49, 1995
14 Yamazaki RK, Shen T, Schade GB. A diet rich in (n-3) fatty acids increase peroxisomal ${\beta}$-oxidation activity and lowers plasma triacylglycerols without inhibiting glutathione-dependent detoxication activities in the rat liver. BBA 920: 62-67, 1987
15 O'Connor TP, Roebuck BD, Peterson FJ, Lokesh B, Kinsella JE, Campbell TC, Effect of dietary omega-3 and omega-6 fatty acids on development of azaserine-induced preneoplastic lesions in rat pancreas. J Natl Cancer Inst 81: 858-863, 1989   DOI   PUBMED
16 Baginski ES, Foa PP, Zak B. Glucose 6-phosphatase. Method Enzymatic Analysis 2: 876-880, 1983
17 Kim Y, Ji SK, Choi H. Modulation of liver microsomal monooxygenase system by dietary n-6/n-3 ratio in rat hepatocarcinogenesis. Nutr Cancer 37 (1): 65-72, 2000   DOI   ScienceOn
18 Misslbeck NG, Campell TC, Roe DA. Effect of ethanol consumption in combination with high and low fat diets on the post initiation phase ofhepatocarcinogenesis. J Nutr 114: 2311-2323, 1984
19 Gonzalez MJ, Schemmel RA, Gray JI, Dugan L, Sheffield LS, Welsch CW. Effect of dietary fat on growth of MCF-7 and MDAMB231 human breast carcinomas in athymic nude mice: relationship between carcinoma growth and lipid peroxidation product levels. Carcinogenesis 12: 1231-1235, 1991   DOI   ScienceOn
20 Chen ZY, Eaton DL. Differential regulation of cytochrome P-450 2B1/2 by phenobarbital in hepatic hyperplastic nodules induced by aflatoxin B1 or diethylnitrosamine plus 2-acetylaminofluorene in male F344 rats. Toxicol Applied Pharmacology 111: 132-144,1991   DOI   ScienceOn
21 Osigo T, Tatematsu M, Tamano S, Tsuda H, Ito N. Comparative effects of carcinogenesis on the induction of placental glutathione S-transferase positive liver nodules in a short-term assay and of hepatocellular carcinogenesis in a long-term assay. Toxicol Pathol 13: 257-265, 1985   DOI   ScienceOn
22 Chan PC, Ferguson KA, Dao TL. Effects of different dietary fats on mammary carcinogenesis. Cancer Res 43: 1079-1083, 1983
23 Klurfeld DM, Kritchevsky D. Update on dietary fat and cancer. PSEBM 183: 287-292, 1986
24 Reddy BS, Sugie S. Effect of different levels of omega-3 and omega-6 fatty acids on azoxymethane-induced colon carcinogenesis in F344 rats. Cancer Res 48: 6642-6647, 1988
25 Misra HP, Fridovich I. Superoxide dismutase: a photochemical augmentation assay. Arch Biochem Biophys 181: 308, 1977   DOI   ScienceOn
26 Kim S. Effects of Vitamin E on the Hepatocellular Chemical Carcinogenesis in Rats. doctoral thesis of Seoul National University, 1996
27 Birt DF. The influence of dietary fat on carcinogenesis: lessons from experimental models. Nutr Rev 48: 1-5, 1990   DOI   ScienceOn
28 Sasagawa T, Kosai K, Ota Y, Mori M, Okita M. Influences of a dietary fatty acid composition on the emergence of glutathione S-transterase-P (GST-P) positive foci in the liver of carcinogentreated rats. Prostaglandins Leukot Essent Fatty Acids 67 (5) : 327-332, 2002   DOI   ScienceOn
29 Tappel AL. Glutathione peroxidase and hydroperoxides. Method Enzymology 52:506-513, 1978   DOI
30 Kim K. Effects of n-3, n-6 fatty acids on the hepatic histological changes and antioxidant enzyme system in the chemical treated rats. doctoral thesis of Seoul National University, 1994
31 Emmanuel F. Cellular biochemistry of the stepwise development of cancer with chemicals: G.H.A. Clows Memorial Lecture. Cancer Res 44: 5463-5474, 1984
32 Welsch CW. Enhancement of mammary tumorigenesis by dietary fat: review of potential mechanisms. Am J Clin Nutr 45: 192-202, 1987
33 Salgado MC, Meton I, Egea M, Baanante IV. Transcriptional regulation of glucose-6-phosphatase catalytic subunit promoter by insulin and glucose in the carnivorous fish, Sparus aurata. J Molecular Endocrinology 33: 783-795, 2004   DOI   ScienceOn
34 Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-275, 1951
35 Fischer MA, Black HS. Modification of membrane composition, eicosanoid metabolism, and immunoresposiveness by dietary omega-3 and omega-6 fatty acid sources, modulators of ultraviolet-carcinogenesis. Photochem Photobiol 54 (3) : 391-387, 1991
36 Oum JI, Effects of vitamin E supplement of com and perilla oils on the cytochrome P-450 contents and lipid peroxide production in hepatocelluar carcinogenesis. Master's thesis of Seoul National University, 1994
37 L' Abbe MR, Trick KD, Beare-rogers JL. Dietary (n-3) fatty acids affect rat heart, liver and aorta protective enzyme activities and lipid peroxidation. J Nutr 121: 1331-1340, 1991
38 Ip C, Carter CA, Ip MM. Requirement of essential fatty acid for mammary tumorigenesis in the rat. Cancer Res 45: 1997-2001, 1985
39 Lee HJ, Kim HK, Choi H. Different sources of ${\omega}$3 fatty acids at the fixed ratio of p/s affect glutathione dependent enzymes in rat hepatocarcinogenesis. Korean J Nutition 36 (8) : 785-792, 2003