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Enhancing Effect of Zingiber Officinale Roscoe Extracts on Mouse Spleen and Macrophage Cells Activation  

Ryu Hye Sook (숙명여자대학교 생활과학대학 식품영양학과)
Kim Jin (숙명여자대학교 생활과학대학 식품영양학과)
Park Sang Chul (서울대학교 체력과학노화연구소)
Kim Hun Sook (숙명여자대학교 생활과학대학 식품영양학과)
Publication Information
Journal of Nutrition and Health / v.37, no.9, 2004 , pp. 780-785 More about this Journal
Abstract
Recently many investigators have initiated searches for immunomodulating substances from natural food sources. Ginger (Zingiber officinale Roscoe) has been used as a raw material in many traditional preparations since the ancient time. This study was performed to investigate the immunomodulative effects of Zingiber officinale Roscoe in mice, using ex vivo experiments. In order to elucidate the immunomodulative effects of Ginger, water extracts of the plant were orally administrated into mice, and isolated splenocytes and macrophages were used as experimental model. In order to identify its ex vivo effect six to seven week old Balb/c mice were fed ad libitum on a chow diet and water extracts of ginger were orally administrated every other day for two weeks at two different concentrations (50 and 500 mg/kg b.w.). After preparing the single cell suspension, the proliferation of splenocytes was determined by MTT assay. The result of ex vivo study showed that the highest proliferation of splenocytes and macrophage activatation was seen in the mice orally administrated at the concentration of 500 mg/kg b. w. of ginger water extracts. In conclusion, this study suggests that ginger extracts nay enhance the immune function by regulating the splenocyte proliferation and cytokine prodution capacity by activated macrophages in mice.
Keywords
ginger extracts; two weeks; immunomodulating; splenocyte proliferation; IL-1$\beta$; IL-6; TNF-$\alpha$;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Nurahman, Zakaria-Runkat, E Prangdimurt and Tejasari. Antioxidant and Immunoenhancement Activites of Ginger (Zingiber officinale Roscoe) Extracts and Compounds in In Vitro and In Vivo Mouse and Human Sytem. Nutraceuticals & Food (8): 96-104, 2003   DOI
2 Ryu HS, Kim HK. Effect of Zingiber officinale Roscoe extracts on mouse Immune Cell. Korean J of Nutrition 37 (1): 23-30, 2004
3 Weiss L. Cell and Tissue Biology, 6th ed. Uraban & Schwarzenbeg, Baltimore, USA, pp.481-538, 1988
4 Ramunans Z, Ian SZ, Robert HB, C. Max L, Paricia JM. In vivo effect of chronic treatment with (met5) -enkephalin on hematological values and natural killer cell activity in athvity in athymic mice. Life Sci 66 (9) : 829-834, 2000   DOI   ScienceOn
5 You sook Lee. Effect of bark Extract on Modulation of Immunocompetence and Irradiation-induced Inflammation Response in Mice. Ph.D. Dissertation Sookmyung Women's University, 2003
6 Starkbaum G. role of cytokines in rheumatoid arthritis. Science & Medicine. March April, pp.6-15, 1998
7 Cooksley VG. Aromatherapy, Englewood Cliffs, N, J, pp.349-350, 1996
8 Matthias W Wichmann, Alfred Ayala and Irshad H. Chaudry, Male sex steroid are responnsbil for depressing depressing macropage immune function after trauma-hemorrhage, The American Physiological Society, pp.C1335-C1340, 1997
9 Roitt I, Brostoff J, Male D. Immunology 4th ed., Mosby, 1996
10 Wagner H. Search for plant derived natural products with immunostimulatory activity. Pure & Appl Chem 66.7: 1271, 1990
11 You sook Lee. Effect of bark Extract on Modulation of Immunocompetence and Irradiation-induced Inflammation Response in Mice. PhD. Dissertation Sookmyung Women's University, 2003
12 Mishell BB, Shigi SM. Selected methods in cellular immunology. 1st ed. San Francisco. WH Freeman and Co. 4.. 1980
13 Adachi Y, Okazaki M, Ohno N, Yadomae T. BioI. Pharm. Bull 17: 1554-1560, 1994   DOI
14 Reubel GH, Bauerfrind R. on the suitability of the MTT assay for evvaluation of mitogenic lymphocyte blastogenesis in swine. Zentrabl Veterinarmed B 36: 35, 1989
15 Thomson AW. The cytokine handbook, Academic Press, 1991
16 Arai S. studies on Function Foods in Japan-state of Art Biosci. Biotechnol. Biochem 60: 9-15, 1996   DOI   ScienceOn
17 James GL. Methods in Immunotoxicology. vol. 2. Wiley-Liss. Inc. 15., 1995
18 Thomson M, Al-Qattan K, Al-Sawan M. The use of ginger (Zingiber officinale Roscoe) as a potential anti-inflammatory and antithrombotic agent. Elsevier Science Ltd 67 (6) : 475-478, 2002
19 Eun-Yi Moon, Seung-Yong Park, Eun-Kyue Park. Influence of Angelicae Gigantis Radix on the Immune System Kor J of Immunol 13: 191, 1991
20 Effect of Zingiber Officinale Roscoe Exteacts on Mice Immune Cell Activation. Korean J of Nutrition 37 (1) : 23-30, 2004
21 Sheo HJ. The antibacterial action of galic, onion, ginger and red pepper juice. J Korean Soc Food Sci Nutr 18 (1) : 94-99, 1999
22 Kuby. immunology. Freeman. 4rd, 2000
23 Kim Jin. Enhancing effect of Paeonia japonica, Houttuynia cordata, and Aster scaber extracts on the immunoreactivity in vivo in mice. Ph.D. Dissertation Sookmyung Women's University, 2003