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Effects of Pine Needle Ethyl Acetate Fraction on Acctylcholine(ACh) and Its Related Enzymes in Brain of Rats  

최진호 (부경대학교 생화학교실)
김대익 (부경대학교 생화학교실)
박시향 (부경대학교 생화학교실)
백승진 (부경대학교 생화학교실)
김남주 (부경대학교 생화학교실)
조원기 (밀양대학교 식품과학과)
김군자 (숙명여자대학교 식품영양학과)
김현숙 (숙명여자대학교 식품영양학과)
Publication Information
Journal of Nutrition and Health / v.37, no.2, 2004 , pp. 95-99 More about this Journal
Abstract
This study was designed to investigate the effects of ethyl acetate(EtOAc) fraction of pine (Pinus densiflora Sieb et Zucc) needle on cholesterol and lipofuscin(LF) accumulations, acetylcholine(ACh) and its related enzyme activities such as choline acetyltransferase(ChAT) and acetylcholinesterase(AChE), and monoamine oxidase-B(MAO-B) activity, which destroyed the catecholamine related neurotransmitters in brain membranes of Sprague- Dawley (SD) rats. Male SD rats were fed basic diets (control group) and experimental diets (EtOAc-25, EtOAc-50 and EtOAc-100) for 45 days. Cholesterol accumulations in mitocholndria and microsomes were significantly inhibited (11.8-12.1% and 9.6-13.0%, respectvely) in EtOAc-50 and EtOAc-100 groups. ACh levels and ChAT activities were significantly increased about 10% in membranes of EtOAc-100 group compared with control group. AChE activities were significantly increased about 8 -12% in membranes of EtOAc-50 and EtOAc-100 groups compared with control group. MAO-B activities were significantly inhibited about 10% in membrane of EtOAc-l00 group compared with control group. These results suggest that ethyl acetate fraction of pine needle may play an effective role in inhibiting cholesterol and improving a membrane fluidity, and learning and memory impairments. (Korean J Nutrition 37(2): 95 -99, 2004)
Keywords
pine(Pinus densiflora); acetylcholine; choline acetyltransferase; monoamine oxidase-${\beta}$; acetylcholinesterase;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
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