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http://dx.doi.org/10.4333/KPS.2009.39.6.411

Haplotype Analysis and Single Nucleotide Polymorphism Frequency of PEPT1 Gene (Exon 5 and 16) in Korean  

Kim, Se-Mi (College of Pharmacy, Institute of Bioequivalence and Bridging Study, Chonnam National University)
Lee, Sang-No (College of Pharmacy, Institute of Bioequivalence and Bridging Study, Chonnam National University)
Kang, Hyun-Ah (Pharmaceutical Research Institute, CJ Cheiljedang Corp)
Cho, Hea-Young (Clinical Trials Management Division, KFDA)
Lee, Il-Kwon (Genome Research Center for Hematopoietic Diseases (GRCHD), Chonnam National University)
Lee, Yong-Bok (College of Pharmacy, Institute of Bioequivalence and Bridging Study, Chonnam National University)
Publication Information
Journal of Pharmaceutical Investigation / v.39, no.6, 2009 , pp. 411-416 More about this Journal
Abstract
The aim of this study was to investigate the frequency of the SNPs on PEPT1 exon 5 and 16 and to analyze haplotype frequency on PEPT1 exon 5 and 16 in Korean population. A total of 519 healthy subjects was genotyped for PEPT1, using pyrosequencing analysis and polymerase chain reaction-based diagnostic tests. Haplotype was statistically inferred using an algorithm based on the expectation-maximization (EM). PEPT1 exon 5 G381A genotyping revealed that the frequency for homozygous wild-type (G/G), heterozygous (G/A) and homozygous mutant-type (A/A) was 30.4, 53.4 and 16.2%, respectively. PEPT1 exon 16 G1287C genotyping revealed that the frequency for homozygous G/G, heterozygous G/C and homozygous C/C type was 88.8, 10.0 and 1.2%, respectively. Based on these genotype data, haplotype analysis between PEPT1 exon 5 G381A and exon 16 G1287C using HapAnalyzer and PL-EM has proceeded. The result has revealed that linkage disequilibrium between alleles is not obvious (|D'|=0.3667).
Keywords
PEPT1 Exon 5 and 16; Genetic polymorphisms; Haplotype; Korean;
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