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http://dx.doi.org/10.4333/KPS.2009.39.1.001

Preparation and Cellular Uptake of Hydrophobic Quantum Dots Encapsulated in Poly-L-Lactic Acid Film  

Lee, Ji-Sook (Center for Chemoinformatics Research, Korea Institute of Science and Technology)
Woo, Kyoung-Ja (Nano-Materials Research Center, Korea Institute of Science and Technology)
Chung, He-Sson (Center for Chemoinformatics Research, Korea Institute of Science and Technology)
Publication Information
Journal of Pharmaceutical Investigation / v.39, no.1, 2009 , pp. 1-6 More about this Journal
Abstract
To overcome the stability problem of hydrophilic quantum dot (Q-dot), cellular uptake of hydrophobic instead of hydrophilic Q-dot was studied in the hope to find a simple method to use Q-dot as a cellular imaging probe. Hydrophobic Q-dot and poly-L-lactic acid (PLLA) were co-dissolved in chloroform to prepare stable films. Due to the cellular compatibility of PLLA, adherent cells were cultured on the film to observe the degree of Q-dot uptake and cytotoxicity of the prepared films. The results show that Q-dots were absorbed into NIH3T3 and EMT6 cells. Cellular uptake was also observed when hydrophobic Q-dots were coated directly on a glass plate. PLLA/Q-dot film and Q-dot coated on glass plate did not show major cytotoxicity. In vivo tumor model was also used to show the uptake of Q-dot from the PLLA/Q-dot film to the tumor site.
Keywords
Quantum-dot; PLLA; Cellular Uptake; hydrophobic; tumor imaging;
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