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http://dx.doi.org/10.4333/KPS.2007.37.1.039

Encapsulation of Plasmid DNA in PLGA Nanoparticles: Effects of Poloxamer and Temperature  

Kang, Hyun-Suk (College of Pharmacy, Chungnam National University)
Ryu, Sang-Hwa (College of Pharmacy, Chungnam National University)
Myung, Chang-Seon (College of Pharmacy, Chungnam National University)
Hwang, Sung-Joo (College of Pharmacy, Chungnam National University)
Park, Jeong-Sook (College of Pharmacy, Chungnam National University)
Publication Information
Journal of Pharmaceutical Investigation / v.37, no.1, 2007 , pp. 39-43 More about this Journal
Abstract
Previously, we have reported that PLGA nanoparticles were prepared for sustained release of water-soluble blue dextran and the particle size, in vitro release pattern and encapsulation were modulated by varying polymers. This study was designed to encapsulate plasmid DNA in PLGA nanoparticles and to investigate the effect of Polymers and temperatures. PLGA nanoparticles were fabricated with poloxamer 188 (P188) or poloxamer 407 (P407) by using spontaneous emulsification solvent diffusion method. As a model plasmid DNA, pCMV-Taq2B/1L-18 was encapsulated in PLGA nanoparticles. Then, the particle size, zeta potential and encapsulation efficiency of nanoparticles containing plasmid DNA were investigated. Particle sizes of PLGA nanoparticles prepared with P188 and P407 were in the range of 200-330 nm and 250-290 nm, respectively. Zeta potentials of nanoparticles were negative regardless of nanoparticle compositions. Encapsulation efficiency of P407 nanoparticles prepared at $30^{\circ}C$ was higher than those at other preparation condition. From the results, the PLGA nanoparticles prepared with poloxamers at different temperature, could modulate the particles size of nanoparticles, and encapsulation efficiency of plasmid DNA.
Keywords
PLGA nanoparticles; Poloxamer; Plasmid DNA; Encapsulation;
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Times Cited By KSCI : 2  (Citation Analysis)
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