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http://dx.doi.org/10.4333/KPS.2004.34.2.095

Effect of Flavonoids on Efflux and Cytotoxicity of Daunomycin  

Chung, Soo-Yeon (College of Pharmacy, Ewha Womans University)
Go, Eun-Jung (College of Pharmacy, Ewha Womans University)
Kim, Na-Hyung (College of Pharmacy, Ewha Womans University)
Lee, Hwa-Jeong (College of Pharmacy, Ewha Womans University)
Publication Information
Journal of Pharmaceutical Investigation / v.34, no.2, 2004 , pp. 95-99 More about this Journal
Abstract
One mechanism which plays a prominent role in development of multi-drug resistance seen in cancer cells is the over-expression of P-glycoprotein (P-gp). It is known that compounds found in vegetables and fruits not only have anticarcinogenic properties but may also modulate P-gp activity. The effect of some dietary components on efflux of daunomycin (DNM), a P-gp substrate, was examined in P-gp over-expressed human uterine sarcoma cell line, MES-SA/DX5. The efflux of DNM from the cells was significantly inhibited by quercetin and verapamil, but not by 1-naphtyl-isothiocyanate (NITC). The $IC_{50}$ values for DNM in MES-SA/DX5 cells were increased by flavonoids (quercetin and fisetin), but not by NITC after 72 hour incubation with dietary constituents. In conclusion, flavonoids may play a role in the modulation of P­-gp activity in human uterine sarcoma cells.
Keywords
P-glycoprotein; Quercetin; Fisetin; 1-Naphthyl-isotiocyanate; Verapamil; Human uterine sarcoma cells;
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