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http://dx.doi.org/10.4333/KPS.2004.34.1.023

Cytotoxic Effect of Urushiol-ethanol Micro-particles on Human Cervical Carcinoma Cells  

Kim, Jin-Woo (College of Medicine, Catholic University)
Ryu, Kyu-Eun (College of Medicine, Catholic University)
Jang, Hong-Seok (College of Medicine, Catholic University)
Ahn, Woong-Shick (College of Medicine, Catholic University)
Choi, Jong-Oh (Chemical Analysis Laboratory, Korea Research Institute of Standards and Science)
Chun, Heung-Jae (College of Medicine, Catholic University)
Publication Information
Journal of Pharmaceutical Investigation / v.34, no.1, 2004 , pp. 23-27 More about this Journal
Abstract
The urushiol-ethanol corpuscle of 320 nM in average particle size was prepared and concentrated by ultra homogenization and centrifugation. The cytotoxic profiles of this particle for use as anti-tumor agent have been evaluated in vitro in cultures of human fibroblasts (MRC-9) and celvical carcinoma cells (CUMC-3). The cytotoxicty assays revealed that the inhibitor effect of $10^{-5}$ M urushiol-ethanol particle on the growth of MRC-9 was hardly detected, while CUMC-3 cells exhibited over 50% of growth inhibition under the same conditions. In addition, a clear multiple-unit ladder pater of apoptotic DNA was observed for the urushiol treated CUMC-3 cells. Thus, the results indicated that urushiol inhibited growth of celvical carcinoma cells by inducing apoptosis, which is a mechanism observed with other typical antitumor agents.
Keywords
Urushiol-ethanol micro-particle; Anti-tumor reagent; Apoptosis;
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