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http://dx.doi.org/10.4333/KPS.2002.32.4.291

Preparation and Evaluation of Titrated Extract of Centella Asiatica Niosome/W/O System Cream for Site Specific Targeting  

Kim, Dong-Woo (College of Pharmacy, Chungnam National University)
Cho, Mi-Hyun (College of Pharmacy, Chungnam National University)
Park, Sun-Young (College of Pharmacy, Chungnam National University)
Lee, Jong-Hwa (College of Pharmacy, Chungnam National University)
Lee, Gye-Won (Dep. of Pharmaceutical Engineering, Konyang University)
Park, Mork-Soon (Dongkook Pharmaceutical Research Institute)
Park, Jin-Kyu (Dongkook Pharmaceutical Research Institute)
Jee, Ung-Kil (College of Pharmacy, Chungnam National University)
Publication Information
Journal of Pharmaceutical Investigation / v.32, no.4, 2002 , pp. 291-297 More about this Journal
Abstract
For preventing and curing the stretching mark, TECA Niosome/W/O system creams were formulated using Titrated Extract of Centella Asiatica (TECA) which is well known for its excellent wound healing effect. The lipid-water partition coefficients and the stabilities of TECA were evaluated and TECA Niosome/W/O system (TECA N/W/O) creams were prepared with different concentrations of cetyl alcohol and ceramide. TECA N/W/O cream was evaluated with respect to their rheological properties, permeation through excised skin of hairless mouse and in vitro and in vivo accumulation in the skin of hairless mouse. In addition, dermal thicknesses of hairless mouse skins were determined following the in vivo application of TECA N/W/O cream and control cream. TECA N/W/O creams showed pseudoplastic flow and hysteresis loop. The permeation of TECA from formulations through excised skin of hairless mouse did not observed. Amount of accumulated drug in the excised skin of hairless mouse was deσeased with an increase in the concentration of cetyl alcohol and showed no relationship with concentration of ceramide. Amount of accumulated drug in formulation A-3 was higher than in niosome suspension and other formulations. In in vivo experiment, amount of accumulated drug in formulation A-2 and A-3 was much higher than that of niosome suspension. Being treated with the N/W/O cream for 8 weeks, the dermal thickness of hairless mouse skin was increased 3.2 times than that of 16 weeks-control group.
Keywords
TECA; niosome; Niosome/W/O system cream;
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1 D.W. Beamer and A.D. Bangham, Large volume liposomes by an ether vaporization method, Biochem. Biophys. Acta, 443, 629 (1976)   DOI   ScienceOn
2 F. Sozoka and D. Papahadijopulos, Procedure for prepara- tion of liposomes with large internal aqueous volume and high capture by reverse-phase evaporation, Proc. Nat. Acad. Sci., 75, 4194 (1978)   DOI   ScienceOn
3 J.L. Rigand, A. Bluzat and S. Bushln, Incorporation of bacteriorhodopsin into large unilamellar liposomes by reverse evaporation, Biochem. Biophys. Res. Commun., 111, 373 (1983)   DOI   ScienceOn
4 F. Olson, C.A. Hunt, F.C. Szoka, W.J. Vail and D. Papahadjopulos, Preparation of liposomes of defended size distribution by extrusion through polycarbonate membranes, Biochem. Biophys. Acta, 557, 9 (1979)   DOI   ScienceOn
5 B. Hercelin, M. Delauany-Vantrou, F. Alamichel, M. Mazza and J.P. Marty, Pharmacokinetic of cutaneous sulconazole nitrate in the hairless rat: absorption, excretion, tissue concentration, Eur. J. Drug Metabol. Pharmacokin., 18, 149 (1993)   DOI   ScienceOn
6 E. Boelsma, C.H. Verhoeven and M. Ponec, Reconstruction of a human skin equivalent using a spontaneously transformed keratinocyte cell line(HaCaT), J. Invest. Dermatol., 112, 489 (1999)   DOI   ScienceOn
7 J.M. Davidson, P.A. Luvalle, O. Zoia, D. Quaglino and M.G. Giro, Ascorbate differentially regulates elastin and collagen biosynthesis in vascular smooth muscle cells and skin fibroblasts by pretranslational mechanism, J. Biochem., 272, 345 (1997)
8 W.J. Dixon, Up & Down Methods, J. Am. Stat. As., 60, 967 (1959)
9 A.D. Bangham, M.M. Standish and J.C. Watkins, Diffusion of univalent ions across the lamellae of swollen phospholipids, J. Mol. Biol., 13, 238 (1965)   DOI
10 M. Ausborn, P. Nuhn and H. Schreier, Stabilization of liposomes by freeze-thaw and lyophilization techniques : problems and opportunities, Eur. J. Pharm. Biopharm., 38, 133 (1992)
11 R.M. Handjani-Vila, A. Riber, B. Rondot and G. Vanler- ghie, Dispersions of lamellar phase of non-ionic lipids in cosmetic products, Int. J. Sci., 1, 303 (1979)
12 A.J. Baille, A.T. Florence, L.F. Hume, G.T. Murihead and A. Rogerson, The preparation and properties of niosomes : non-ionic surfactant vesicles, J. Pharm. Pharmacol., 37, 863 (1985)   DOI
13 T. Yoshika, K. Ikeuchi, M. Hashida, S. Mauranishi and H. Sezeki, Prolonged release of bleomycin from parenteral gelatin sphere-in-oil-in-water multiple emulsion, Chem. Pharm. Bull., 30, 1408 (1982)   DOI   ScienceOn
14 C.H,. Huang, Studies on phosphatidylcholine vesicles formu- lation and physical characteristic, Biochemistry, 8, 344 (1969)   DOI   ScienceOn
15 R. Ganapathi and A. Krishnan, Effect of cholesterol content of liposomes on encapsulation, efflux and toxicity of adriamycin, Biochem. Pharmacol., 33, 698 (1984)   DOI   ScienceOn
16 De M. Luca, J.L. Grossiord, C. Vaution and M. Seiler, A stable W/O/W multiple emulsion, Cosmet. Toiletries., 105, 65 (1990)
17 S. Fukushima, K. Juni and M. Makano, Preparation of drug release from W/O/W type double emulsion containing anticancer agents, Chem. Pharm. Bull., 31, 4048 (1983)   DOI   ScienceOn
18 L. Grossiord, M. Seiler and F. Pusieux, Apport des analyses rheologiques dans letude demulsions multiples, Rheol. Acta, 32, 168 (1993)   DOI   ScienceOn
19 S. Teruyuki and K. Yasuhiko, Ceramide derivatives as therapeutic agents, Exp. Opin. Ther. Patents., 8, 1673 (1998)
20 K. Kakemi, T. Arita, R. Hori and R. Konish, Absorption and excretion of drugs XXX. Absorption of barbituric acid derivatives from rat stomach, Chem. Pharm. Bull., 15, 1534 (1967)   DOI   ScienceOn