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Chemical Properties and Biological Activity of Garlic (Allium sativum L.) Shoots  

Kang, Min-Jung (Namhae Garlic Research Institute)
Yoon, Hwan-Sik (Namhae Garlic Research Institute)
Shin, Jung-Hye (Namhae Garlic Research Institute)
Publication Information
Journal of agriculture & life science / v.46, no.4, 2012 , pp. 129-139 More about this Journal
Abstract
This study was carried out to evaluate the chemical properties and functional characteristics, such as general composition and bioactivity compounds contents of fresh and blanched (at $95^{\circ}C$, for 5min) garlic shoot from Namhae. Also, evaluated antioxidant and antimicrobial activities of water and ethanol extract of fresh and blanched garlic shoot. The moisture content of fresh garlic steam was $ 85.14{\pm}0.35%$, crude protein and crude lipid were $0.79{\pm}0.26%$ and $2.96{\pm}0.03%$ respectively. Vitamin C content was higher in fresh garlic shoot ($7.07{\pm}0.84mg/100g$) than blanched. Total phenol and total chlorophyll contents were respectively $16.93{\pm}1.17mM/g$ and $6.70{\pm}0.46mg/g$ in fresh garlic shoot. Allicin content of blanched garlic shoot was $128.63{\pm}1.59mM/g$. This content was 1.82 times higher than the fresh garlic shoot. Total pyruvate content was higher in fresh garlic shoot ($24.63{\pm}1.59mM/g$), but thiosulfinate was higher in fresh garlic shoot. Total flavonoide was the highest in water extract of blanched garlic shoot ($3.67{\pm}0.00mM/g$). ABTS radical scavenging activity of water extract form blanched garlic shoot was $85.09{\pm}0.28%$, which was higher than the other extracts. NO radical scavenging activity of ethanol extract from blanched garlic shoot was significantly higher than the extracts from fresh garlic shoot. Antibacterial activity to S. aureus, S. enterica, B. cereus and E. coli was only indicated in water extract of fresh garlic shoot.
Keywords
Antioxidant activity; Chlorophyll; Garlic; Garlic shoot; Total phenol;
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1 Lee, E. J., K. S. Kim, H. Y. Jung, D. H. Kim, and H. D. Jang. 2005. Antioxidant activities of garlic (Allium sativum L.) with growing districts. Food Sci. Biotechnol. 14: 123-130.
2 Lee, H. K., H. J, Park, and S. N. Kim. 2007. Explanation on food composition table (Section 7. Revised edition). Rural resources development institute.
3 Lee, M. K., J. S. Park, and H. S. Na. 2005. Proximate compositions of green garlic powder and microbiological properties of bread with green garlic. Korean J. Food Preserv. 12: 95-100.
4 Lee, S. J., J. H. Shin, M. J. Kang, W. J. Jung, J. H. Ryu, R. J. Kim, and N. J. Sung. 2010. Antioxidants activity of aged red garlic. J. Life Sci. 20: 775-781.   DOI
5 Lee, S. J., J. H. Ryu, R. J. Kim, H. J. Lee, and N. J. Sung. 2010. Effect of removed peel from sweet persimmon on nutritional ingredients and antioxidant activities. J. Korean Soc. Food Sci. Nut. 39: 1495-1502.   DOI
6 Lee, S. O., H. J. Lee, M. H, Yu, H. G. Im, and I. S. Lee, 2005. Total poly phenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung island. Korean J. Food Sci. Technol 37: 233-240.
7 Mazelis, M. and L. Crews. 1968. Purification of the alliin lyase of garlic, Allium sativum L. Biochem. J. 108: 725-730.
8 Moreno, M. I. N., M. I. Isla, A. R. Sampietro, and M. A. Vattuone. 2000. Comparison of the free radical scavenging activity of propolis from several region of Argentina. J. Ethnopharmacol. 71: 109-114.   DOI   ScienceOn
9 Nuttakaan, L., R. Viboon, C. Nantaya, and M. G. Janusz. 2006. Quantitative evaluation of the antioxidant properties of garlic and shallot preparations. Nutrition 22: 266-274.   DOI   ScienceOn
10 Park, P. S. and M. Y. Lee. 1992. The effects of onion and garlic on copper -phenanthroline complex induced DNA degradation. J. Korean Soc. Food Nutr. 21: 367-371.
11 Re, R., N. Pellegrini, A. Pannala, M. Yang, and C. Rice-Evans. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26: 1231-1237.   DOI   ScienceOn
12 Rho, S. N. and J. H. Han. 2000. Cytotoxicity of garlic and onion methanol extract on human lung cancer cell lines. J. Korean Soc. Food Sci. Nutr. 29: 870- 874
13 Saleem, Z. M. and K. S. Al-Delaimy. 1982. Inhibition of Bacillus cereus by garlic extracts. J. Food Prot. 45: 1007-1009.
14 Schwimmer, S. and W. J. Weston. 1961. Onion flavor and order, enzymatic development of pyruvic acid in onion as a measure of pungency. J. Anal. Food Chem. 9: 301-304.   DOI
15 Sheo, H. J. 1999. Effects of garlic on the blood lipids and other serum components in rats. J. Korean Soc. Food Sci. Nutr. 28: 1339-1348.
16 Shin, J. H., J. C. Ju, O. C. Kwon S. M. Yang, and S. J. Lee. 2004. Physicochemical activities of garlic from different area. Korea J. Food Nutr. 17: 237-245.
17 Son, S. G., S. M. Je, S. Y. Woo, K. O. Byun, Y. J. Kang, and B. S. Kwang. 2006. Physiological differences of Ilex rotunda and Illicium anisatum under low light intensities. J. Agric. Forest Meteorol. 8: 61-67.
18 Song, H. S. and K. Y. Moon. 2006. In vitro antioxidant activity profiles of $\beta$-glucans isolated from yeast Saccharomyces cerevisiae and mutant Saccharomyces cerevisiae IS2. Food Sci. Biotechnol. 15, 437-440.
19 Stoll, A. and E. Seebeck. 1951. Chemical investigations on alliin, the specific principle of garlic. Adv. Enzymol. 11: 377-399.
20 Teng, S. S. and B. H. Chen. 1999. Formation of pyrochlorophylls and their derivatives in pinach leaves during heating. Food Chem. 65: 367-373.   DOI   ScienceOn
21 Yang, S. Y., S. O. Yu, T. C. Kim, B. W. Kim, Y. J. Park, J. Y. Cho, Y. M. Kim, and B. G. Heo. 2005. Compare to the component contents and anti-oxidation activities between Goheung native garlic variety and introductions. J. Korean Soc. Plant People Environ. 8: 1-5.
22 Ahn, M. S. 1999. A study on a changes in physicochemical properties of vegetables by Korean traditional cooking methods. Korean J. Diet. Cult. 14: 177-188.
23 AOAC. 1990. Official Methods of Analysis. 13th ed. Association of Official Analytical Chemists, Washington, DC., USA. p 132.
24 Belguith, H., F. Kthiri, A. Chati, A. Abu Sofah, J. Ben Hamida, and A. Ladoulsi. 2010. Inhibitory effect of aqueous garlic extract (Allium sativum) on some isolated Smonella serovars. Afr. J. Microbiol. Res. 4: 328-338.
25 Benzie, I. F. and J. J. Strain. 1996. The ferric reducing ability of plasma (FRAP) as a measure of " antioxidant power" : the FRAP assay. Anal. Biochem. J. 239: 70-76.   DOI   ScienceOn
26 Chung, J. Y. and C. S. Kim. 2008. Antioxidant activities of domestic garlic (Allium sativum L.) stems from different areas. J. Korean Soc. Food Sci. Nutr. 37: 972-978.   DOI
27 Chung, J. Y. and C. S. Kim. 2009. Antioxidant activities of domestic garlic (Allium sativum L.) stems and garlic bulbs according to cooking methods. J. Korean Soc. Food Sci. Nutr. 38: 188-194.   DOI
28 Chung, K. S., J. Y. Kim, and Y. M. Kim. 2003. Comparison of antibacterial activities of garlic juice and heat-treated garlic juice. Korean J. Food Sci. Technol. 35: 540-543.
29 Chung, M. J., J. H. Shin, S. J. Lee, S. K. Hong H. J. Kang, and N. J. Sung. 1998. Chemical compounds of wild and cultivated horned rampion, Phyteuma japonicum Miq. Korean J. Food Nutr. 11: 437-443.
30 Freeman, G. G. and F. Mcbreen. 1973. A rapid spectrophotometric methods of determination of thiosulfinate in onion and its significance in flavor studies. Biochem. Soc. Trans. 1: 1150-1154.
31 Gutfinger, T. 1981. Polyphenols olive oils. J. Am. Oil Chem. Soc. 58: 966-968.   DOI   ScienceOn
32 Hong, M. S. 1992. The culture of food in Korea. Kyomoonsa, Seoul. p 79.
33 Jain, R. C. and C. R. Vyas. 1975. Garlic in alloxan-induced diabetic rabbits. Am J. Clin. Nutr. 28: 6865-6874.
34 Jeon, H. Y., J. K. Kim, D. B. Seo, and S. J. Lee. 2009. Effect of chlorophyll a on UVB-induced cellular responses and type I pN collagen synthesis in vitro. Korean J. Food Sci. Technol. 41: 700-705.
35 Jo, J. S. 1990. Food materials. Gijeunyungusa, Seoul, Korea. p 154-155.
36 Kim, B. M., Y. J., Oh, and S. B. Lim. 2009. Physicochemical characteristics of garlic from daejeong jeju and major cultivation areas in korea. Korean J. Soc. Food Cookery Sci. 15: 59-66.
37 Kim, G. T., and D. S. Lee 2001. Processing and storage of spinach production using Cook-chill and sous vide methods. J. Korea Soc. Food Sci. Nutr. 30: 1095-1101.
38 Kim, H. J., G. J. Kim, T. W. Jun, E. S. Lee, Y. S. Lee, and O. K. Han. 2002. Hepatoprotective effects of semisulcospira libertina and garlic on the liver damage induced by carbon tetrachloride in rats. J. Korean Soc. Food Sci. Nutr. 31: 58-520.
39 Kim, M. H., S. Y. Kim, W. S. Shin, and J. S. Lee. 2003. Antimicrobial activity of garlic juice against Escherichia coli O157:H7. Korean J. Food Sci. Technol. 35: 752-755.
40 Kim, M. J., S. J. Lee, R. J. Kim, B. Y. Jeong, and N. J. Sung. 2011. Mineral content and antioxidants activity of Portulaca oleracea. J. Life Sci. 21: 1393- 1400.   DOI
41 Kim, S. H., J. O. Kim, S. H. Lee, K. Y. Park, H. J. Park, and H. Y. Chung. 1991. Antimutagenic compounds identified from the chloroform fraction of garlic (Allium sativum). J. Korean Soc. Food Nutr. 20: 253-259.
42 Kyung, K. H. 2006. Growth inhibitory activity of sulfer compounds of garlic against pathogenic microorganisms. J. Fd. Hyg. Safety 21: 145-152.
43 Lawson, L. D., S. G. Wood, and B. G. Hughes. 1991. HPLC analysis of allicin and other thiosulfinates in garlic glove homogenates. Planta. Med. 57: 263-270.   DOI