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Use of Agrobacterium for the Genetic Transformation of Trichoderma harzianum  

Park, Hee-Sung (Department of Biotechnology, Catholic University of Daegu)
Shin, Dong-Il (Department of Biotechnology, Catholic University of Daegu)
Publication Information
Journal of agriculture & life science / v.45, no.6, 2011 , pp. 175-181 More about this Journal
Abstract
Effective Agrobacterium-mediated transformation of Trichoderma harzianum could be achieved using the $Al_2O_3$ particles-abraded mycelia pellets. Transformation efficiency, as percents for the number of hygromcin-resistant mycelia pellets out of total pellets tested, was about 20 in average for $Al_2O_3$ experiment. No transformed mycelium was obtained from the intact mycelia pellets. After second round of antibiotics selection, DNA integration of hygromycin resistant gene and the expression of target gene could be confirmed by PCR and RT PCR, respectively. This is the first report of Agrobacterium-mediated T. harzianum transformation.
Keywords
Agrobacterium; Mycelium; Transformation; Trichoderma harzianum;
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