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A Simple Method of RNA Extraction from Apple Stem and Leaf Tissues via Heating  

Duong, Thanh Van (Department of Biotechnology, Catholic University of Daegu)
Shin, Dong-Il (Department of Biotechnology, Catholic University of Daegu)
Park, Hee-Sung (Department of Biotechnology, Catholic University of Daegu)
Publication Information
Journal of agriculture & life science / v.44, no.5, 2010 , pp. 75-79 More about this Journal
Abstract
Apple, one of the most important fruit crops world-widely, is required for rapid, cost-effective and sensitive virus detection for its better productivity. RT-PCR is extensively employed for apple virus diagnosis, but the technique requires complete tissue homogenization which is time consuming and laborious. In this study, heating-based RNA extraction method was developed and proven to effectively work for stem tissues slightly better than for leaf tissues. In RT-PCR, almost identical results were generated from the use of RNA extracts from both tissues.
Keywords
Apple virus; Stem; Leaf; Heating; RNA; RT-PCR;
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