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http://dx.doi.org/10.5757/JKVS.2008.17.6.549

Effects of Plasma on the Surface of Protein Chip Plates  

Hyun, J.W. (Department of Applied Physics, Dankook University)
Kim, N.Y. (Department of Applied Physics, Dankook University)
Publication Information
Journal of the Korean Vacuum Society / v.17, no.6, 2008 , pp. 549-554 More about this Journal
Abstract
Nickel Chloride coated protein chip plates were developed by using a spin coating method after $H_2$ plasma treatment. The adsorption ability of histidine tagged protein was investigated at various times of plasma treatment. The properties of the nickel chloride and protein on the surface of the slides were assayed using particle size analysis and the extent of the protein adsorption was determined by using a bio imaging analyzer system. The results show that the ability of protein adsorption decreased as increasing the time of $H_2$ plasma treatment. The mechanism on the ability of protein adsorption at the plate surface is discussed on results and discussions. The results also suggest that the surface stabilization of protein chip plates treated by plasma technology may be applicable in biosensor markets.
Keywords
Protein chip plates; $H_2$ plasma treatment; Histidine tagged protein; Ability of protein adsorption;
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