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http://dx.doi.org/10.7841/ksbbj.2016.31.3.186

A Newly Developed Analytical and Semi-preparative Enantiomer Separation of Fluoxetine using Polysaccharide-derived Chiral Stationary Phases by High Performance Liquid Chromatography  

Kim, Seok Jin (Gwangju Science Academy)
Nam, Kyung Wook (Gwangju Science Academy)
Park, Bohyun (Gwangju Science Academy)
Islam, Md. Fokhrul (College of Pharmacy, Chosun University)
Lee, Wonjae (College of Pharmacy, Chosun University)
Publication Information
KSBB Journal / v.31, no.3, 2016 , pp. 186-191 More about this Journal
Abstract
A liquid chromatographic method for the enantiomer separation of fluoxetine was performed using covalently bonded and coated type polysaccharide-derived chiral stationary phases (CSPs). The degree of enantioseparation is affected by the used CSPs and mobile phases. The performance of Chiralpak IC was superior to the other CSPs used in this study. Out of various solvent composition and additives, the greatest separation and resolution was observed using Chiralpak IC with mobile phase of 2-propanol in hexane with diethylamine as an additive. Semi-preparative separation of fluoxetine was performed on the analytical Chiralpak IC column to obtain (R)- and (S)-fluoxetine enantiomer with high chemical and optical purity. From the overall study, the developed liquid chromatographic method on polysaccharide-derived CSPs is expected to be very useful for the enantiomer separation of fluoxetine.
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