Biosynthesis of Lactate-containing Polyhydroxyalkanoates in Recombinant Escherichia coli by Employing New CoA Transferases
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Kim, You Jin
(Department of Environmental Engineering and Energy, Myongji University)
Chae, Cheol Gi (Department of Environmental Engineering and Energy, Myongji University) Kang, Kyoung Hee (Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology) Oh, Young Hoon (Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology) Joo, Jeong Chan (Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology) Song, Bong Keun (Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology) Lee, Sang Yup (Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST) Park, Si Jae (Department of Environmental Engineering and Energy, Myongji University) |
1 | Oh, Y. H., I. Y. Eom, J. C. Joo, J. H. Yu, B. K. Song, S. H. Lee, S. H. Hong, and S. J. Park (2015) Recent advances in development of biomass pretreatment technologies used in biorefinery for the production of bio-based fuels, chemicals and polymers. Korean J. Chem. Eng. 32: 1945-1959. DOI |
2 | Lee, S. Y. (1996) Bacterial polyhydroxyalkanoates. Biotechnol. Bioeng. 49: 1-14. DOI |
3 | Steinbuchel, A. and H. E. Valentin (1995) Diversity of bacterial polyhydroxyalkanoic acid. FEMS Microbiol. Lett. 128: 219-228. DOI |
4 | Jung, Y. K., T. Y. Kim, S. J. Park, and S. Y. Lee (2010) Metabolic engineering of Escherichia coli for the production of polylactic acid and its copolymers. Biotechnol. Bioeng. 105: 161-171. DOI |
5 | Park, S. J., S. Y. Lee, T. W. Kim, Y. K. Jung, and T. H. Yang (2012) Biosynthesis of lactate containing polyesters by metabolically engineered bacteria. Biotechnol. J. 7:199-212. DOI |
6 | Park, S. J., T. W. Lee, S. C. Lim, T. W. Kim, H. Lee, M. K. Kim, S. H. Lee, B. K. Song, and S. Y. Lee (2012) Biosynthesis of polyhydroxyalkanoates containing 2-hydroxybutyrate from unrelated carbon source by metabolically engineered Escherichia coli. Appl. Microbiol. Biotech. 93:273-283. DOI |
7 | Park, S. J., Y. A. Jang, H. Lee, A. R. Park, J. E. Yang, J. Shin, Y. H. Oh, B. K. Song, J. Jegal, S. H. Lee, and S. Y. Lee (2013) Metabolic engineering of Ralstonia eutropha for the biosynthesis of 2-hydroxyacid containing polyhydroxyalkanoates (PHAs). Metab. Eng. 20: 20-28. DOI |
8 | Park, S. J., K. H. Kang, H. Lee, A. R. Park, J. E. Yang, Y. H. Oh, B. K. Song, J. Jegal, S. H. Lee, and S. Y. Lee (2013) Propionyl-CoA dependent biosynthesis of 2-hydroxybutyrate containing polyhydroxyalkanoates in metabolically engineered Escherichia coli. J. Biotechnol. 165: 93-98. DOI |
9 | Yang, T. H., T. W. Kim, H. O. Kang, S. H. Lee, E. J. Lee, S. C. Lim, S. O. Oh, A. J. Song, S. J. Park, and S. Y. Lee (2010) Biosynthesis of polylactic acid and its copolymers using evolved propionate CoA transferase and PHA synthase. Biotechnol. Bioeng. 105: 150-160. DOI |
10 | Yang, T. H., Y. K. Jung, H. O. Kang, T. W. Kim, S. J. Park, and S. Y. Lee (2011) Tailor-made type II Pseudomonas PHA synthases and their use for the biosynthesis of polylactic acid and its copolymer in recombinant Escherichia coli. Appl. Microbiol. Biotechnol. 90: 603-614. DOI |
11 | Yang, J. E., S. Y. Choi, J. H. Shin, S. J. Park, and S. Y. Lee (2013) Microbial production of lactate-containing polyesters. Microb. Biotechnol. 6: 621-636. |
12 | Braunegg, G., B. Sonnleitner, and R. M. Lafferty (1978) A rapid gas chromatographic method for the determination of poly-bhydroxybutyric acid in microbial biomass. Eur. J. Appl. Microbiol. Biotechnol. 6: 29-37. DOI |
13 | Choi, J., S. Y. Lee, K. Shin, W. G. Lee, S. J. Park, H. N. Chang, and Y. K. Chang (2002) Pilot scale production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by fed-batch culture of recombinant Escherichia coli. Biotechnol. Bioprocess Eng. 7: 371-374. DOI |
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