Browse > Article
http://dx.doi.org/10.7841/ksbbj.2015.30.6.275

Antioxidant Activity of the Halophyte Ligustrum japonicum  

Baek, Seung Oh (Division of Marine Bioscience, Korea Maritime & Ocean University)
Kim, Hojun (Division of Marine Bioscience, Korea Maritime & Ocean University)
Jeong, Heejeong (Ocean Science & Technology School, Korea Maritime & Ocean University)
Ju, Eunsin (Division of Marine Bioscience, Korea Maritime & Ocean University)
Kong, Chang-Suk (Department of Food and Nutrition, College of Medical & Life Sciences, Silla University)
Seo, Youngwan (Division of Marine Bioscience, Korea Maritime & Ocean University)
Publication Information
KSBB Journal / v.30, no.6, 2015 , pp. 275-282 More about this Journal
Abstract
Dried samples of Ligustrum japonicum were extracted twice: with methylene chloride and with methanol (MeOH), respectively. The combined crude extracts were successively fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH), and water fractions by liquid-liquid partition. Antioxidant activities of crude extract and its solvent fractions were evaluated by measuring authentic $ONOO^-$, and $ONOO^-$ generated from 3-morpholinsydnonimine (SIN-1) as well as degree of occurrence of intracellular ROS in HT 1080 cells, and genomic DNA oxidation. The 85% aq.MeOH and n-BuOH fractions exhibited the good antioxidant activity. Further purification of the 85% aq.MeOH fracition led to the isolation of Oleanolic acid (1), Maslinic acid (2), and Ursolic acid (3). All compounds showed the significant antioxidant effects in all assay systems.
Keywords
Ligustrum japonicum; Antioxidant; Reactive oxygen species (ROS); Peroxynitrite;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 D'Abrosca, B., A. Fiorentino, P. Monaco, P. Oriano, and S. Pacifico (2006) Annurcoic acid: A new antioxidant ursane triterpene from fruits of cv. Annurca apple. Food Chem. 98: 285-290.   DOI
2 Ramachandran, S. and N. R. Prasad (2008) Effect of ursolic acid, a triterpenoid antioxidant, on ultraviolet-B radiation-induced cytotoxicity, lipid peroxidation and DNA damage in human lymphocytes. Chem. Biol. Interact. 176: 99-107.   DOI
3 Montilla, M. P., A. Agil, M. C. Navarro, M. I. Jimnez, A. Garca- Granados, A. Parra, and M. M. Cabo (2003) Antioxidant activity of Maslinic acid, a triterpene derivative obtained from Olea europaea. Planta Med. 69: 470-472.   DOI
4 Liu, C.-H., M.-H. Yen, S.-F. Tsang, K.-H. Gan, H.-Y. Hsu, and C.- N. Lin (2010) Antioxidant triterpenoids from the stems of Momordica charantia. Food Chem. 118: 751-756.   DOI
5 Qiao, A., Y. Wang, L. Xiang, Z. Zhang, and X. He (2014) Triterpenoids of sour jujube show pronounced inhibitory effect on human tumor cells and antioxidant activity. Fitoterapia 98: 137-142.   DOI
6 Qiao, A., Y. Wang, L. Xiang, Z. Zhang, and X. He (2015) Novel triterpenoids isolated from hawthorn berries functioned as antioxidant and antiproliferative activities. J. Funct. Foods 13: 308-313.   DOI
7 Power, S. T. and M. J. Jackson (2008) Exercise-induced oxidative stress: Cellular mechanisms and impact on muscle force production. Physiol. Rev. 88: 1243-1276.   DOI
8 Sung, C., Y. Hsu, C. Chen, Y. Lin, and C. Wu (2013) Oxidative stress and nucleic acid oxidation in patients with chronic kidney disease. Oxid. Med. Cell Longev. 2013: 1-15.
9 Barbieri, E. and Sestili, P. (2012) Reactive oxygen species in skeletal muscle signaling. J. Signal Transduct. 2012: 1-17.
10 da Silva, F. M., A. Marques, and A. Chaveiro (2010) Reactive oxygen species: A double-edged sword in reproduction. Open Vet. Sci. J. 4: 127-133.   DOI
11 Alfadda, A. A. and Sallam, R. M. (2012) Reactive oxygen species in health and disease. J. Biomed. Biotechnol. 1-14.
12 Gutierrez, J. and M. S. V. Elkind (2012) Chronic inflammatory diseases and stroke: Evidence for heterogeneous mechanisms. Ann. Neurol. 72: S6-S7.
13 Choi, U., D.-H. shin, Y-S Chang, and J. I Shin (1992) Screening of natrura antioxidant from plant and their antioxidative effect. Korea J. Food Sci. Technol. 24: 142-148.
14 Karre, L., K. Lopez, and K. J. Getty (2013) Natural antioxidants in meat and poultry products. Meat Sci. 94: 220-227.   DOI
15 Jo, J.-O. and I.-C. Jung (2006) Phenolic compounds of Ligustrum japonicum leaves. J. Korea Soc. Food Sci. Nutr. 35: 713-720.   DOI
16 Kim, Y. J., Y. R. Lee, J. W. Cheon, and H. S. Lee (2010) Antiaging effect of Ligustrum japonicum extract in the human fibroblast cells. J. Soc. Cosmet. Scientists Korea 36: 295-301.
17 Sung, S. Y., E. S. Kim, K. Y. Lee, M. K. Lee, Y. C. Kim (2006) A new neuroprotective compound of Ligustrum japonicum leaves. Planta Med. 72: 62-64.   DOI
18 Okimoto, Y., A. Watanabe, E. Niki, T. Yamashita, and N. Noguchi (2000) A novel fluorescent probe diphenyl-1-pyrenylphosphine to follow lipid peroxidation in cell membranes. FEBS Lett. 474: 137-140..   DOI
19 Papoti, V. T., K. Pegklidou, E. Perifantsi, N. Nenadis, V. J. Demopoulos, and M. Z. Tsimidou (2011) Antioxidant and aldose reductase inhibition activity of Ligustrum japonicum and Olea europaea L. leaf extracts. Eur. J. Lipid Sci. Technol. 113: 876-885.   DOI
20 Kooy, N. W., J. A. Royall, H. Ischiropoulos, and J. S. Beckman (1994) Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free Radic. Biol. Med. 16:149-156.   DOI
21 Miline, L., P. Nicotera, S. Orrenius, and M. Burkitt (1993) Effects of glutathione and chelating agents on copper-mediated DNA oxidation: Prooxidant and antioxidant properties of glutathione. Arch. Biochem. Biophys. 304: 102-109.   DOI
22 Werner, S., S. Nebojsa, W. Robert, S. Robert, and K. Olaf (2003) Complete assignments of $^{1}H$ and $^{13}C$ NMR resonances of oleanolic acid, $18{\alpha}$-oleanolic acid, ursolic acid and their 11-oxo derivatives. Magn Reson Chem 41: 636-638.   DOI
23 Ibrahim, T. B. and O. S. Francis (2013) Ubiquitous ursolic acid: a potential pentacyclic triterpene natural product. J. Pharmacogn. Phytochem. 2: 214-222.
24 Woo, K. W., J. Y. Han, S. U. Choi, K. H. Kim, and K. R. Lee (2014) Triterpenes from Perilla frutescens var. acuta and their cytotoxic activity. Natural Product Sciences 20: 71-75.
25 Choudhary, A., A. K. Mittal, M. Radhika, D. Tripathy, A. Chatterjee, U. C. Banerjee, and I. P. Singh (2013) Two new stereoisomeric antioxidant triterpenes from Potentilla fulgens. Fitoterapia 91: 290-297.   DOI