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http://dx.doi.org/10.7841/ksbbj.2015.30.5.239

Characterization and Antimicrobial Activity against Gardnerella vaginalis of Vaginal Lactobacillus spp. Isolated from Korean Women  

Kim, YongGyeong (Department of Biological Engineering, Inha University)
Kang, Chang-Ho (Department of Biological Engineering, Inha University)
Shin, YuJin (Department of Biological Engineering, Inha University)
Paek, Nam-Soo (MEDIOGEN, Co., Ltd.)
So, Jae-Seong (Department of Biological Engineering, Inha University)
Publication Information
KSBB Journal / v.30, no.5, 2015 , pp. 239-244 More about this Journal
Abstract
Bacterial vaginosis (BV) is caused by microbial imbalance of the vaginal ecosystem and overgrowth of anaerobic bacteria. The antibiotic treatment often results in very high recurrence of BV because it disturbs the vaginal ecosystem. The high recurrence rates suggest a need for alternative therapeutic methods and probiotics are being recognized as alternative or additional treatment method for BV. The purpose of this study was to investigate how human vaginal isolates of Lactobacillus spp. inhibit the BV-associated pathogen Gardnerella vaginalis. Results show that selected strains significantly reduced the viability of G. vaginalis. Among these selected strains KLB410 and KLB416 were further selected based on acid/bile tolerance and identified through 16S rRNA gene sequencing being Lactobacillus plantarum. Further studies are underway to demonstrate that the selected strain can be applied as potential probiotics for recovering vaginal ecosystem.
Keywords
Bacterial vaginosis; Gardnerella vaginalis; Probiotics; Lactobacillus spp.;
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1 Wiesenfeld, H. C, S. L. Hillier, M. A. Krohn, D. V. Landers, and R. L. Sweet (2003) Bacterial vaginosis is a strong predictor of Neisseria gonorrhoeae and Chlamydia trachomatis infection. Clin. Infect. Dis. 36: 663-668.   DOI
2 Bradshaw, C. S., A. N. Morton, J. Hocking, S. M. Garland, M. B. Morris, L. M. Moss, L. B. Horvath, I. Kuzevska, and C. K. Fairley (2006) High recurrence rates of bacterial vaginosis over the course of 12 months after oral metronidazole therapy and factors associated with recurrence. J. Infect. Dis. 193: 1478-1486.   DOI
3 Falagas, M. E., G. I. Betsi, and S. Athanasiou (2007) Probiotics for the treatment of women with bacterial vaginosis. Clin. Microbiol. Infect. 13: 657-664.   DOI
4 Borge, S., J. Silva, and P. Teixeira (2013) The role of lactobacilli and probiotics in maintaining vaginal health. Arch. Gynecol. Obstet. 289: 479-489.
5 Bastani, P., A. Homayouni, S. Ziyadi, and V. G. Tabrizian (2012) Dairy Probiotic Foods and Bacterial Vaginosis: A Review on Mechanism of Action. pp. 445-456. In: Probiotics, Rigobelo, E. C. (Ed.), Intech., USA.
6 Chang, C. E., S. I. Pavlova, L. Tao, E. Kim, S. C. Kim, H. S. Yun and J. S. So (2002) Molecular Identification of Vaginal Lactobacillus spp. Isolated from Korean Women. J. Microbiol. Biotechnol. 12: 312-317.
7 Guo, X. H., J. M. Kim, H. M. Nam, S. Y. Park and J. M. Kim (2010) Screening lactic acid bacteria from swine origins for multistrain probiotics based on in vitro functional properties. Anaerobe 16: 321-326.   DOI
8 Kassa, I. A., M. Hamze, D. Hober, N. E. Chihib and D. Drider (2014) Identification of Vaginal Lactobacilli with Potential Probiotic Propertied Isolated from Women in North Lebanon. Microb. Ecol. 67: 722-734.   DOI
9 Borchardt, K. A., B. S. Adly, R. F. Smith, J. Eapen, and C. B. Beal (1989) Importance of Gardnerella vaginalis as an aetiological agent in bacterial vaginosis. Genitourin. Med. 65: 285.
10 Machado, A., K. K. Jefferson, and N. Cerca (2013) Interactions between Lactobacillus crispatus and Bacterial Vaginosis (BV)-associated bacterial species in initial attachment and biofilm formation. Int. J. Mol. Sci. 14: 12004-12012.   DOI   ScienceOn
11 Dunne, C., L. O'Mahony, L. Murphy, G. Thornton, D. Morrissey, S. O'Halloran, M. Feeney, S. Flynn, G. Fitzgerald, C. Daly, B. Kiely, G. C. O'Sullivan, F. Shanahan, and J K. Collins (2001) In vitro selection criteria for probiotic bacteria of human origin: Correlation with in vivo findings. Am. J. Clin. Nutr. 73: 386S-392S.   DOI
12 Tasi, C. C., H. Y. Hsih, H. H. Chiu, Y. Y. Lai, J. H. Liu, B. Yu (2005) Antagonistic activity against Salmonella infection in vitro and in vivo for two Lactobacillus strains from swine and poultry. Int. J. Food Microbiol. 102: 185-194.   DOI   ScienceOn
13 Mishra, V., and D. N. Prasad (2005) Application of in vitro methods for selection of Lactobacillus casei strains as potential probiotics. Int. J. Food Microbiol. 103: 109-115.   DOI
14 Gilliland, S. E., T. E. Staley, and L. J. Bush (1984) Importance of bile tolerance of Lactobacillus acidophilus used as a dietary adjunct. J. Dairy Sci. 67: 3045-5301.   DOI
15 Lin, W. H., C. F. Hwangb, L. W. Chenc, and H. Y. Tsen (2006) Viable counts, characteristic evaluation for commercial lactic acid bacteria products. Food Microbiol. 23: 74-81.   DOI
16 Del, R. B. Sgorbati, M. Moglioli and D. Palenzona (2000) Adhesion, autoaggregation and hydrophobicity of 13 strains of bafidobacterium longum. Lett. Appl. Microbiol. 31:438-442.   DOI
17 Ministry of Food and Drug Safety, The standard for health functional food. http://www.mfds.go.kr. (2014).
18 Kos, B., J. Suskoviae, S. Vukoviae, M. Simpraga, J. Frece and S. Matosiae (2003) Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. J. Appl. Microbiol. 94: 981-987.   DOI
19 Malik, S., M. I. Petrova, I. J. J. Claes, T. L. A. Verhoeven, P. Busschaert, M. Vaneechoutte, B. Lievens, I. Lambrichts, R. J. Siezen, J. Balzarini, J. Vanderleyden and S. Lebeer (2013) The highly autoaggregative and adhesive phenotype of the vaginal Lactobacillus plantarum strain CMPG5300 is sortase dependent. Appl. Environ. Microbiol. 79: 4576-4585.   DOI
20 Kleerebezem, M., J. Boekhorst, R. van Kranenburg, D. Molenaar, O. P. Kuipers, R. Leer, R. Tarchini, S. A. Peters, H. M. Sandbrink, M. W. E. J. Fiers, W. Stiekema, R. M. K. Lankhorst, P. A. Bron, S. M. Hoffer, M. N. N. Groot, R. Kerkhoven, M. de Vries, B. Ursing, W. M. de Vos, and R. J. Siezen (2003) Complete genome sequence of Lactobacillus plantarum WCFS1. Proc. Natl. Acad. Sci. USA 100: 1990-1995.   DOI
21 Verstraelena, H., and A. C Senok (2005) Vaginal lactobacilli, probiotics, and IVF. Reprod. Biomed. Online 11: 674-675.   DOI
22 Mastromarino, P., B. Vitali, and L. Mosca (2013) Bacterial vaginosis: A review on clinical trials with probiotics. New Microbiologica 36: 229-238.
23 Chen, K. C., P. S. Forsyth, T. M. Buchanan, and K. K. Holmes (1979) Amine content of vaginal fluid from untreated and treated patients with nonspecific vaginitis. J. Clin. Invest. 63: 826-835.