Browse > Article
http://dx.doi.org/10.7841/ksbbj.2015.30.4.191

Anthocyanin Analysis of Pressure-extracted Korean Blueberry Juice and in vitro Anti-inflammatory in RAW267.4 Cell line  

Choi, Moon-Hee (Major in Cosmetic Engineering, Department of Chemical Engineering, Graduate School of Engineering, Chosun University)
Jeon, Young-Jin (Department of Pharmacology, Chosun University)
Shin, Hyun-Jae (Major in Cosmetic Engineering, Department of Chemical Engineering, Graduate School of Engineering, Chosun University)
Publication Information
KSBB Journal / v.30, no.4, 2015 , pp. 191-196 More about this Journal
Abstract
Blueberry juice possesses rich-procyanidins and - anthocyanidin, comprised a group of with numerous health benefits such as protection against coronary heart disease, detoxification, and obesity. Blueberry (Vaccinium virgatum) juice extracts were analyzed and separated by an HPLC method for the purpose of the separation and quantification in polyphenolic groups. In specific HPLC conditions, a binary mobile phase consisting of formic acid: water (10:90, v/v, solvent A) and formic acid: water: acetonitrile (10:60:30, v/v/v, solvent B) was utilized and it is detected at 546 nm wavelength. The phenolic contents of the extracts are determined using Folin-Ciocalteu phenol reagent. In order to test anti-inflammation activity assay, after producing nitric oxide (NO) in lipopolysaccharide activated RAW 264.7 cells, at concentration of $20-500{\mu}g/mL$ it reduced to NO production at a dose-dependent manner. Importantly, cytotoxicity assay with up to $500{\mu}g/mL$ of the extract from blueberry juice showed ~100% cell viability for RAW264.7 cell line. Therefore, Korean blueberry juice might have potential as anti-oxidant and antiinflammation agents.
Keywords
Blueberry; Anthocyanin; Polyphenol; Nitric oxide; Cytotoxicity; Antioxidant activity;
Citations & Related Records
Times Cited By KSCI : 11  (Citation Analysis)
연도 인용수 순위
1 Fraga, C. G., M. Galleano, S. V. Verstraeten, and P. I. Oteiza (2010) Basic biochemical mechanisms behind the health benefits of polyphenols. Mol. Aspects Med. 31: 435-445.   DOI
2 Choi, S. Y., H. S. Cho, and N. J. Sung (2006) The antioxidative and nitrite scavenging ability of solvent extracts from wild grape (Vitis coignetiea) skin. J. Korean Soc. Food Sci. Nutr. 35: 961-966.   DOI
3 Jeong, C. H., C. W. Jang, K. Y. Lee, I. H. Kim, and K.W. Shim (2012) Chemical components and anti-oxidant activities of black currant. Korean J. Food Preserv. 19: 263-270.   DOI   ScienceOn
4 Lee, H. R., J. Y. Park, I. W. Hwang, S. K. Kim, J. U. Choi, and S. K. Chung (2008) Antioxidant activity and total phenolic contents of grape juice products in the Korean market. Korean J. Food Preserv. 15: 445-449.
5 Okada, Y., Ishimaru A., Suzuki R., and T. Okuyama (2004) A new phloroglucinol derivative from the brown algae Eisenia bicyclis: potential for the effective treatment of diabetic complications. J. Nat. Prod. 67: 103-105.   DOI
6 Choi, M. H., M. J. Kim, Y. J. Jeon, and H. J. Shin (2014) Quality changes of fresh vegetable and fruit juice by various juicers. KSBB J. 29: 145-154.   DOI
7 Benvenuti, S., F .Pellati, M .Melegari, and D. Bertelli (2004) Polyphenols, anthocyanins, ascorbic acid, and radical scavenging activity of reubus, ribes, and aronia. J. Food Sci. 69: 164-169.
8 Moon, H. K., S. W. Lee, and J. K. Kim (2013) Physicochemical and quality characteristics of the Korean and American blueberries. Korean J. Food Preserv. 20: 524-531.   DOI   ScienceOn
9 Basu, A., M. Rhone, and T. J. Lyons (2010) Berries: Emerging impact on cardiovascular health. Nutr. Rev. 68: 168-177.   DOI
10 Byeon, S. E., J. Y. Chung, Y. G. Lee, and B. H. Kim (2008) In vitro and in vivo anti-inflammatory effects of taheebo, a water extract from the inner bark of Tabebuia avellanedae. J. Ethnopharmacol. 119: 145-152.   DOI
11 Lee, S. H., S. P. Park, O. J. Park, and Y. M. Kim (2012) Effects of resveratrol on migration and proliferation in HT-29 colon cancer cells. KSBB J. 27: 289-294.   DOI   ScienceOn
12 Park, H. J., E. S. Han, D. K. Park, C. Lee, and K. W. Lee (2010) An extract of Phellinus linteus grown on germinated brown rice inhibits inflammation markers in RAW 264.7 macrophages by suppressing inflammatory cytokines, chemokines, and mediators and upregulating antioxidant activity. J. Med. Food 13: 1468-1477.   DOI
13 Choi, K. H., H. H. Nam, and B. K. Choo (2013) Effect of five Korean native taraxacum on antioxidant activity and nitric oxide production inhibitory activity. Korean J. Med. Crop. 21: 191-196.   DOI   ScienceOn
14 Garbacki, N., M. Tits, L. Angenot, and J. Damas (2004) Inhibitory effects of proanthocyanidins from Ribes nigrum leaves on carrageen in acute inflammatory reactions induced in rats. BMC Phamacol. 25: 1-9.
15 Van der Sluis A., M. Dekker, A. de Jager, and W. M. F. Jongen (2001) Activity and concentration of polyphenolic antioxidants in apple: effect of cultivar, harvest year, and storage conditions. J. Agric. Food Chem. 49: 3606-3613.   DOI
16 Jeong, C. H., S. G. Choi, and H. J. Heo (2008) Analysis of nutritional compositions and antioxidative activities of Korean commercial blueberry and raspberry. Korean J. Soc. Food Sci. Nutr. 37: 1375-1381.   DOI
17 Hwang, S. J., W. B. Yoon, O. H. Lee, S. J. Cha, and J. D. Kim (2014) Radical-scavenging-linked antioxidant activities of extracts from black chokeberry and blueberry cultivated in Korea. Food Chem. 146: 71-77.   DOI
18 Jeong, H. R., Y. N. Jo, J. H. Jeong, H. J. Kim, and J. H. Ho (2012) Nutritional composition and in vitro antioxidant activities of blueberry (Vaccinium ashei) leaf. Korean J. Food Preserv. 19: 604-610.   DOI   ScienceOn
19 Matchett, M. D., S. L. MacKinnon, M. I. Sweeney, K. T. Gottschall- Pass, and R. A. R. Hurta (2006) Inhibition of matrix metalloproteinase activity in DU145 human prostate cancer 56 cells by flavonoids from lowbush blueberry (Vaccinium angustifolium): Possible roles for protein kinase C and mitogen activated protein-kinasemediated events. J. Nutr. Biochem.17: 117-125.   DOI
20 Kwak, H. Y., S. J. Lee, D. Y. Lee, L. Jung, N. H. Bae, S. Y. Hong, G. W. Kim, and N. I. Baek (2008) Cytotoxic and anti-inflammatory activities of lipids from the Nuruk (Rhizopus oryzae KSD-815) J. Korean Soc. Appl. Biol. Chem. 51: 142-147.