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http://dx.doi.org/10.7841/ksbbj.2015.30.4.141

Development of a Validated Determination of Methylsulfonylmethane in Dietary Supplement by Gas Chromatography  

Park, Sang-Wook (Hazardous Substances Analysis Division, Gwangju Regional Food & Drug Administration)
Lee, Wonjae (College of Pharmacy, Chosun University)
Publication Information
KSBB Journal / v.30, no.4, 2015 , pp. 141-147 More about this Journal
Abstract
The convenient determination of methylsulfonylmethane (MSM) for a commercially available dietary supplement was developed using gas chromatography (GC)-flame ionization detector (FID). Chromatography was performed on a capillary column ($0.32mm\;I.D{\times}30m$, $0.25{\mu}m$) coated with dimethylpolysiloxane using diethylene glycol methyl ether as an internal standard. The performance characteristics of GC were evaluated in terms of selectivity, linearity, precision, accuracy, recovery, limit of detection (LOD) and limit of quantification (LOQ). The calibration curve was highly linear (the coefficient of determination: 0.9979) within the concentration range of $10.0{\sim}800.0{\mu}g/mL$ for MSM. The recoveries for three fortified concentrations were 96.7~97.1%, 96.6~97.3% and 96.8~97.2%, respectively. The LOD and LOQ of the method were $0.29{\mu}g/mL$ and $0.97{\mu}g/mL$, respectively. All obtained results were acceptable according to the guidelines of the Association of Official Analytical Chemists for dietary supplements. Thus, the validated analytical method using the GC-FID system is suitable for the determination of MSM in dietary supplement formulations for quality control.
Keywords
Dietary supplement; Methylsulfonylmethane; Gas chromatography;
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  • Reference
1 Korea's Ministry of Food and Drug Safety, Information for health functionl food. http://www.foodnara.go.kr/hfoodi/. (2008).
2 Natasha, D. R., T. L. Lathem, L. M. Rodriguez-R, J. M. Barazesh, B. E. Anderson, A. J. Beyersdorf, L. D. Ziemba, M. Bergin, A. Nenes, and K. T. Konstantinidis (2012) Microbiome of the upper troposphere: Species composition and prevalence, effects of tropical storms, and atmospheric implications. Natasha Leon-Rodriguez. 110: 2575-2580.
3 Kim, L. S., L. J. Axelrod, P. Howard and N. Buratovich (2006) Efficacy of methylsulfonylmethane (MSM) in osteoarthritis pain of the knee: a pilot clinical trial. Osteoarthr. Cartil. 14: 286-294.   DOI
4 Herschler, R. J. (1985) Dietary and pharmaceutical uses of methylsulfonylmethane and compositions comprising it. USP 4,514,421.
5 Kalman, D. S., F. Samantha, R. S. Andrew, R. K. Diane and J. B. Richard (2012) Influence of methylsulfonylmethane on markers of exercise recovery and performance in healthy men: a pilot study. J. Int. Soc. Sports Nutr. 9: 46-56.   DOI
6 Barmaki, S., S. Bohlooli, F. Khoshkhahesh and R. B. Nakhostin (2012) Effect of methylsulfonylmethane supplementation on exercise - Induced muscle damage and total antioxidant capacity. J. Artic. Randomized Control Trial. 52: 170-174.
7 Usha, P. R and M. U. R. Naidu (2004) Randomised, double-blind, parallel, placebo-controlled study of oral glucosamine, methylsulfonylmethane and their combination in osteoarthritis. Clin. Drug Invest. 24: 353-363.   DOI
8 Takeuchi, A., S. Yamamoto, R. Narai, M. Nishida, M. Yashiki, N. Sakui, and A. Namera (2010) Determination of dimethyl sulfoxide and dimethyl sulfone in urine by gas chromatography-mass spectrometry after preparation using 2,2-dimethoxypropane. Biomed. Chromatogr. 24: 465-471.
9 Satinsky, D., M. Pospilov, and R. Sladkovsk (2014) A new gas chromatography method for quality control of methylsulfonylmethane content in multicomponent dietary supplements. Food Anal. Methods 7: 1118-1122.   DOI
10 Association of Official Analytical Chemists. AOAC guidelines for single laboratory validation of chemical methods for dietary supplements and botanicals. https://www.yumpu.com/en/document/view/11700995/aoac-guidelines-for-single-laboratory-validationof-chemical.htm (2000).
11 Ferreira, A. C. S., P. Rodrigues, T. Hogg, and P. G. De Pinho (2003) Influence of some technological parameters on the formation of dimethyl sulfide, 2-mercaptoethanol, methionol, and dimethyl sulfone in port wines. J. Agric. Food Chem. 51; 727-732.   DOI
12 Korea's Ministry of Food and Drug Safety, Notice #2013-186, "Standard and specification for health functional food". http://www.mfds.go.kr/daegu/index.do?mid=26&seq=20854&cmd=v (2013).
13 Prokop-Prigge, K. A., E. Thaler, C. J. Wysockia, and G. Preti (2014) Identification of volatile organic compounds in human cerumen. J. Chromatogr. B, 953: 48-52.