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http://dx.doi.org/10.7841/ksbbj.2014.29.3.205

Effect of Mobile Phase Additive on Enantiomer Resolution for Chiral Amines on Polysaccharide-derived Chiral Stationary Phases by High Performance Liquid Chromatography  

Paik, Man-Jeong (College of Pharmacy, Sunchon National University)
Yoon, Hye-Ran (College of Pharmacy, Duksung Women's University)
Lee, Wonjae (College of Pharmacy, Chosun University)
Publication Information
KSBB Journal / v.29, no.3, 2014 , pp. 205-209 More about this Journal
Abstract
Chromatographic enantiomer resolution of chiral amines was performed on several covalently immobilized and coated chiral stationary phases (CSPs) based on polysaccharide derivatives under the mobile phase conditions containing base or acid or acid/base additive. The chromatographic parameters including separation factors and capacity factors were greatly influenced by the nature of the mobile phase containing base or acid or salt additive as well as the used CSPs. When 0.05% triethylamine/0.05% trifluoroacetic acid as an additive in the mobile phase was used on all CSPs in this study, the greatest enantiomer resolution was observed except for Chiralpak AD. Also, it was shown that the change of base additive into acid or salt in the mobile phase may directly affect chiral recognition mechanisms between the chiral selectors and analytes occurring during enantiomer separation, resulting in the change of elution orders.
Keywords
Chiral amine; Chiral stationary phase; Enantiomer resolution; Mobile phase additive;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
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