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http://dx.doi.org/10.7841/ksbbj.2013.28.6.423

Enantiomer Separation of α-Amino Acid Esters as Nitrobenzoxadiazole Derivatives Using Chiral Columns  

Yun, Won Nam (College of Pharmacy, Chosun University)
Kim, Ji Yeon (Department of Food and Drug, Graduate School, Chosun University)
Lee, Wonjae (College of Pharmacy, Chosun University)
Publication Information
KSBB Journal / v.28, no.6, 2013 , pp. 423-427 More about this Journal
Abstract
A new convenient derivatization method of ${\alpha}$-amino acid esters as nitrobenzoxadiazole (NBD) derivatives for chiral resolution was introduced and the enantiomer separation of ${\alpha}$-amino acid esters as NBD derivatives was performed by normal HPLC using chiral columns based on polysaccharide derivatives. The NBD derivatives were readily prepared by stirring NBD-Cl and ${\alpha}$-amino acid methyl ester HCl with $NaHCO_3$ in ethanol. The performance of Chiralpak IA was superior to the other chiral stationary phases for enantiomer resolution of NBD derivatives of several ${\alpha}$-amino acid methyl esters. Owing to fluorescence detection as well as strong UV absorption, it is expected that the convenient analytical method developed in this study will be very useful for enantiomer separation of ${\alpha}$-amino acid esters as NBD derivatives on polysaccharide-derived chiral columns.
Keywords
Enantiomer separation; Amino acid ester; NBD derivative; Chiral column;
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Times Cited By KSCI : 3  (Citation Analysis)
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