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http://dx.doi.org/10.7841/ksbbj.2013.28.6.394

Ethanol Extract of Astragalus membranaceus Bunge Induces Aquaporin-3 Expression in HaCaT Cells  

Park, Hyun-Chul (Saimdang Cosmetics Co., Ltd., R&D Center)
Kim, Hee-Taek (Dept. of Oriental Medical Opthalmology & Otolaryngology & Dermatology, Semyung University Oriental Medical Hospital)
Ha, Hun-Young (Dept. of Natural Medicine Resources, Semyung University)
Lee, Pyeong-Jae (Dept. of Natural Medicine Resources, Semyung University)
Yoon, Kyung-Sup (Saimdang Cosmetics Co., Ltd., R&D Center)
Publication Information
KSBB Journal / v.28, no.6, 2013 , pp. 394-399 More about this Journal
Abstract
Astragalus membranaceus Bunge is used in herbal medicine in Eastern Asian countries including Korea. In this study, we assessed the effects of A. membranaceus extract (AM) on the aquaporin-3 (AQP3) protein expression in HaCaT cells. AM did not affect viability of HaCaT cells. AQP3 expression and cell migration seem to be maximal at $100{\mu}g/mL$ concentration. Epidermal growth factor receptor (EGFR) kinase inhibitor, PD153035, blocked AM-induced AQP3 expression and cell migration. In addition, an 80% ethanol extracts of herbal prescription, SinhyoTakleesan (ST), which is composed of A. membranaceus, Angelicae gigantis, Glycyrrhiza glabra Linne, and Lonicera japonica Flos also induced AQP3 expression at $20{\mu}g/mL$ in HaCaT cells. Collectively, these results suggest that AM induce AQP3 expression via EGFR pathway.
Keywords
Astragalus membranaceus; SinhyoTakleesan; Aquaporin-3; Keratinocyte; EGFR;
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